Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production
- Univ. of Delaware, Newark, DE (United States); University of Michigan
Methanol is an attractive and broadly available substrate for large-scale bioproduction of fuels and chemicals. It contains more energy and electrons per carbon than carbohydrates and can be cheaply produced from natural gas. Synthetic methylotrophy refers to the development of non-native methylotrophs such as Escherichia coli and Corynebacterium glutamicum to utilize methanol as a carbon source. Furthermore, we discuss recent advances in engineering these industrial hosts to assimilate methanol for growth and chemicals production through the introduction of the ribulose monophosphate (RuMP) cycle. In addition, we present novel strategies based on flux coupling and adaptive laboratory evolution to engineer new strains that can grow exclusively on methanol.
- Research Organization:
- Univ. of Delaware, Newark, DE (United States)
- Sponsoring Organization:
- USDOE Advanced Research Projects Agency - Energy (ARPA-E)
- Grant/Contract Number:
- AR0000432
- OSTI ID:
- 1747974
- Alternate ID(s):
- OSTI ID: 1778468
- Journal Information:
- Current Opinion in Biotechnology, Journal Name: Current Opinion in Biotechnology Vol. 59; ISSN 0958-1669
- Publisher:
- ElsevierCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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