Microfluidic platform for multiplexed detection in single cells and methods thereof

Wu, Meiye; Singh, Anup K.

Title: Microfluidic platform for multiplexed detection in single cells and methods thereof

Patent · Tue Aug 04 00:00:00 EDT 2020

OSTI ID:1735100

Wu, Meiye; Singh, Anup K.

The present invention relates to a microfluidic device and platform configured to conduct multiplexed analysis within the device. In particular, the device allows multiple targets to be detected on a single-cell level. Also provided are methods of performing multiplexed analyses to detect one or more target nucleic acids, proteins, and post-translational modifications.

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Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: NA0003525

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Number(s):: 10,731,205

Application Number:: 15/925,389

OSTI ID:: 1735100

Resource Relation:: Patent File Date: 03/19/2018

Country of Publication:: United States

Language:: English

References (93)

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Planar micromixer Fiechtner, Gregory J.; Singh, Anup K.; Wiedenman, Boyd J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7344681 US Patent Document 7,344,681	patent	March 2008
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Devices, systems, and methods for conducting sandwich assays using sedimentation Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8945914 US Patent Document 8,945,914	patent	February 2015
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Electrokinetic concentration of charged molecules Singh, Anup K.; Neyer, David W.; Schoeniger, Joseph S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6428666 US Patent Document 6,428,666	patent	August 2002
Single-cell protein analysis Wu, Meiye; Singh, Anup K. Current Opinion in Biotechnology, Vol. 23, Issue 1, p. 83-88 https://doi.org/10.1016/j.copbio.2011.11.023	journal	February 2012
Dielectrokinetic chromatography devices Chirica, Gabriela S.; Fiechtner, Gregory J.; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8911606 US Patent Document 8,911,606	patent	December 2014
Microfluidic devices and methods including porous polymer monoliths Hatch, Anson V.; Sommer, Gregory J.; Singh, Anup K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9201069 US Patent Document 9,201,069	patent	December 2015
Microfluidic Chip for High-Throughput Perfusion-Based Three-Dimensional Cell Culture Wu, Min-Hsien; Wang, Shih-Siou; Tsai, Wan-Chen https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20120231976 US Patent Application 13/241123; 20120231976	patent-application	September 2012

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