Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002

Dahlgren, Kelsey K.; Gates, Colin; Lee, Thomas; Cameron, Jeffrey C.

doi:10.1007/s11120-020-00806-y

Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002

Journal Article · Sun Dec 06 00:00:00 EST 2020 · Photosynthesis Research

DOI:https://doi.org/10.1007/s11120-020-00806-y· OSTI ID:1734387

Dahlgren, Kelsey K.; Gates, Colin; Lee, Thomas;

Abstract

Cyanobacteria possess unique intracellular organization. Many proteomic studies have examined different features of cyanobacteria to learn about the intracellular structures and their respective functions. While these studies have made great progress in understanding cyanobacterial physiology, the conventional fractionation methods used to purify cellular structures have limitations; specifically, certain regions of cells cannot be purified with existing fractionation methods. Proximity-based proteomics techniques were developed to overcome the limitations of biochemical fractionation for proteomics. Proximity-based proteomics relies on spatiotemporal protein labeling followed by mass spectrometry of the labeled proteins to determine the proteome of the region of interest. We performed proximity-based proteomics in the cyanobacterium Synechococcus sp. PCC 7002 with the APEX2 enzyme, an engineered ascorbate peroxidase. We determined the proteome of the thylakoid lumen, a region of the cell that has remained challenging to study with existing methods, using a translational fusion between APEX2 and PsbU, a lumenal subunit of photosystem II. Our results demonstrate the power of APEX2 as a tool to study the cell biology of intracellular features and processes, including photosystem II assembly in cyanobacteria, with enhanced spatiotemporal resolution.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308; SC0019306

OSTI ID:: 1734387

Alternate ID(s):: OSTI ID: 1755713

Report Number(s):: NREL/JA--2800-78686; PII: 806

Journal Information:: Photosynthesis Research, Journal Name: Photosynthesis Research Journal Issue: 2 Vol. 147; ISSN 0166-8595

Publisher:: SpringerCopyright Statement

Country of Publication:: Netherlands

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
APEX2
cyanobacteria
photosynthesis
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Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002

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