In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

Book · Tue Nov 10 04:00:00 EST 2020

OSTI ID:1721680

Liang, Yiran ^[1]; Truong, Thy ^[2]; Zhu, Ying ^[1]; Kelly, Ryan T. ^[3]

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1. BATTELLE (PACIFIC NW LAB)
2. Brigham Young University
3. BRIGHAM YOUNG UNIVERSITY

Leukemic stem cells are highly dynamic and heterogeneous. Analysis of leukemic stem cells at the single cell level should provide a wealth of insights that would not be possible using bulk measurements. Mass spectrometry (MS)-based proteomic workflows can quantify hundreds or thousands of proteins from a biological sample and has proven invaluable for biomedical research, but samples comprising large numbers of cells are typically required due to limited sensitivity. Recent developments in sample processing, chromatographic separations and MS instrumentation are now extending in-depth proteome profiling to single mammalian cells. We describe specific techniques that increase the sensitivity of single-cell proteomics by orders of magnitude, enabling the promise of single cell proteomics to become a reality. We anticipate such techniques could significantly advance our understanding of leukemic stem cells.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1721680
• Report Number(s): PNNL-SA-148793
• Country of Publication: United States
• Language: English