Inorganic semiconductor quantum dots as a saturated excitation (SAX) probe for subdiffraction imaging

Singh, Avinash Kumar; Burkhow, Sadie; Wijesooriya, Chamari S.; Boote, Brett W.; Petrich, Jacob W.; Smith, Emily

doi:10.1002/cptc.202000195

Title: Inorganic semiconductor quantum dots as a saturated excitation (SAX) probe for subdiffraction imaging

Journal Article · Tue Oct 13 00:00:00 EDT 2020 · ChemPhotoChem

DOI:https://doi.org/10.1002/cptc.202000195· OSTI ID:1712675

Singh, Avinash Kumar ^[1]; Burkhow, Sadie ^[2]; Wijesooriya, Chamari S. ^[3]; Boote, Brett W. ^[2]; Petrich, Jacob W. ^[2]; Smith, Emily ^[2]

Ames Lab., Ames, IA (United States)
Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
Iowa State Univ., Ames, IA (United States)

The photoluminescence (PL) saturation of CdSe/ZnS core/shell inorganic semiconductor quantum dots (QDs) and its utility as a probe for saturated excitation (SAX) microscopy are reported. Under saturating excitation power densities, the PL signal was demodulated and recorded at harmonics of the fundamental frequency. For commercially available Qdot® 655 ITK™ QDs, the power density required to achieve saturation was dependent upon the local environment of the QDs. For QDs deposited and dried on a glass substrate, the excitation power density required for PL saturation was less than 1 kW/cm². Compared to this, saturation of PL for QDs dispersed in water required an excitation power density greater than 200 kW/cm². This observation is manifested as a limitation in the imaging of hydrated samples, as demonstrated for HeLa cells labelled with biotinylated-phalloidin followed by labelling with streptavidin-coated QDs. Finally as saturation affects the obtained spatial resolution in several imaging formats, including confocal imaging, the provided data will aid in obtaining the optimal spatial resolution when using QD probes to image biological samples.

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Research Organization:: Ames Lab., Ames, IA (United States)

Sponsoring Organization:: USDOE; National Science Foundation (NSF)

Grant/Contract Number:: AC02-07CH11358; 1709099

OSTI ID:: 1712675

Report Number(s):: IS-J-10,289

Journal Information:: ChemPhotoChem, Vol. 5, Issue 3; ISSN 2367-0932

Publisher:: Wiley - European Chemical Societies PublishingCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
bioimaging
cell imaging
quantum dots
saturation
semiconductors
sub-diffraction imaging

Title: Inorganic semiconductor quantum dots as a saturated excitation (SAX) probe for subdiffraction imaging

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