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Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18

Journal Article · · mSphere
 [1];  [2];  [3];  [4];  [5];  [6];
  1. Molecular Biology Interdepartmental Doctoral Program, University of California, Los Angeles, Los Angeles, California, USA
  2. Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  3. Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA
  4. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  5. Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  6. Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
+ Show Author Affiliations

Bacteriophages are useful nonantibiotic therapeutics for bacterial infections as well as threats to industries utilizing bacterial agents. This study identified Phaeobacter virus MD18 , a phage antagonist of Phaeobacter inhibens , a bacterium with promising use as a probiotic for aquatic farming industries. Genomic analysis suggested that Phaeobacter phage MD18 has evolved to enhance its replication in P. inhibens by adopting favorable tRNA genes as well as through genomic sequence adaptation to resemble host codon usage. Lastly, a high-throughput analysis of P. inhibens transposon insertion mutants identified genes that modulate host susceptibility to phage MD18 and implicated the type IV pilus as the likely receptor recognized for adsorption. This study marks the first characterization of the relationship between P. inhibens and an environmentally sampled phage, which informs our understanding of natural threats to the bacterium and may promote the development of novel phage technologies for genetic manipulation of this host.

Research Organization:
Marine Biological Laboratory, Woods Hole, MA (United States)
Sponsoring Organization:
GGMI; National Science Foundation (NSF); Simons Foundation; USDOE; USDOE Office of Science (SC)
Grant/Contract Number:
SC0016127
OSTI ID:
1698206
Alternate ID(s):
OSTI ID: 1816479
Journal Information:
mSphere, Journal Name: mSphere Journal Issue: 6 Vol. 5; ISSN 2379-5042
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

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