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Title: Source of 12C in Calvin–Benson cycle intermediates and isoprene emitted from plant leaves fed with 13CO2

Journal Article · · Biochemical Journal
DOI:https://doi.org/10.1042/bcj20200480· OSTI ID:1682247
ORCiD logo [1];  [1];  [1];  [1]
  1. Michigan State Univ., East Lansing, MI (United States)
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Feeding 14CO2 was crucial to uncovering the path of carbon in photosynthesis. Feeding 13CO2 to photosynthesizing leaves emitting isoprene has been used to develop hypotheses about the sources of carbon for the methylerythritol 4-phosphate pathway, which makes the precursors for terpene synthesis in chloroplasts and bacteria. Both photosynthesis and isoprene studies found that products label very quickly (<10 min) up to 80–90% but the last 10–20% of labeling requires hours indicating a source of 12C during photosynthesis and isoprene emission. Furthermore, studies with isoprene showed that the proportion of slow label could vary significantly. This was interpreted as a variable contribution of carbon from sources other than the Calvin–Benson cycle (CBC) feeding the methylerythritol 4-phosphate pathway. Here, we measured the degree of label in isoprene and photosynthetic metabolites 20 min after beginning to feed 13CO2. Isoprene labeling was the same as labeling of photosynthesis intermediates. High temperature reduced the label in isoprene and photosynthesis intermediates by the same amount indicating no role for alternative carbon sources for isoprene. A model assuming glucose, fructose, and/or sucrose reenters the CBC as ribulose 5-phosphate through a cytosolic shunt involving glucose 6-phosphate dehydrogenase was consistent with the observations.

Research Organization:
Univ. of Wisconsin, Madison, WI (United States); Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
SC0018409; FG02-91ER20021
OSTI ID:
1682247
Alternate ID(s):
OSTI ID: 1735827
Journal Information:
Biochemical Journal, Vol. 477, Issue 17; ISSN 0264-6021
Publisher:
Biochemical SocietyCopyright Statement
Country of Publication:
United States
Language:
English

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Linked Research (1)

Figures / Tables (8)

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Calvin cycle
glucose 6-phosphate shunt
isoprene
MEcDP
oxidative pentose phosphate pathway