Controlling Drug Absorption, Release, and Erosion of Photopatterned Protein Engineered Hydrogels

Wang, Yao; Delgado-Fukushima, Erika; Fu, Richard X.; Doerk, Gregory S.; Monclare, Jin Kim

doi:10.1021/acs.biomac.0c00616

Controlling Drug Absorption, Release, and Erosion of Photopatterned Protein Engineered Hydrogels

Journal Article · Tue Jul 28 00:00:00 EDT 2020 · Biomacromolecules

DOI:https://doi.org/10.1021/acs.biomac.0c00616· OSTI ID:1679965

Wang, Yao ^[1]; Delgado-Fukushima, Erika ^[1]; Fu, Richard X. ^[2]; ^[3]; ^[1]

New York Univ. (NYU), NY (United States)
Army Research Lab., Adelphi, MD (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States). Center for Functional Nanomaterials (CFN)

A protein-engineered triblock copolymer hydrogel composed of two self-assembling domains (SADs) has been fabricated by a photoactivatable diazirine group followed by ultraviolet (UV)-mediated crosslinking. The photocrosslinkable protein polymer CEC-D has been patterned into various features including different micrometer-scale stripes by using lithographic techniques. The patterned hydrogels are important for encapsulation of small molecules where a photopatterned fraction of 50% is optimal for maximum absorption. Stripe-patterned CEC-D_100–100 exhibits slightly lower swelling ratios, an 8.9 times lower erosion profile, and a 2.6-fold higher drug release compared to the unpatterned hydrogel control, CEC-D0. Our studies demonstrate the potential of photocrosslinkable protein polymer hydrogels to be used as scaffolds for therapeutic delivery of small molecules. Furthermore, through photolithographic techniques on the protein hydrogel, a variety of functionalities can be achieved by patterning different features enabling the mimicry of biological systems.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: US Army Research Office (ARO); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704

OSTI ID:: 1679965

Report Number(s):: BNL--219978-2020-JAAM

Journal Information:: Biomacromolecules, Journal Name: Biomacromolecules Journal Issue: 9 Vol. 21; ISSN 1525-7797

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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