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Structural insights into the mechanism of rhodopsin phosphodiesterase

Dataset ·
DOI:https://doi.org/10.11577/1679953· OSTI ID:1679953

Crystal structures of TMD of Rhodopsin phosphodiesterase (Rh-PDE). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.

Research Organization:
Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); University of Tokyo
Sponsoring Organization:
University of Tokyo
DOE Contract Number:
AC02-05CH11231
OSTI ID:
1679953
Report Number(s):
CXIDB ID 171
Country of Publication:
United States
Language:
English

References (1)

Structural insights into the mechanism of rhodopsin phosphodiesterase journal January 2020

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