Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry

Zhou, Mowei; Lantz, Carter; Brown, Kyle A.; Ge, Ying; Paša-Tolić, Ljiljana; Loo, Joseph A.; Lermyte, Frederik

doi:10.1039/D0SC04392C

Title: Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry

Journal Article · Tue Oct 20 00:00:00 EDT 2020 · Chemical Science

DOI:https://doi.org/10.1039/D0SC04392C· OSTI ID:1678783

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Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, USA
Department of Chemistry and Biochemistry, Department of Biological Chemistry, University of California-Los Angeles, Los Angeles, USA
Department of Chemistry, University of Wisconsin–Madison, Madison, USA
Department of Chemistry, University of Wisconsin–Madison, Madison, USA, Department of Cell and Regenerative Biology
Department of Chemistry, Institute of Chemistry and Biochemistry, Technical University of Darmstadt, 64287 Darmstadt, Germany

In biology, it can be argued that if the genome contains the script for a cell's life cycle, then the proteome constitutes an ensemble cast of actors that brings these instructions to life. Their interactions with each other, co-factors, ligands, substrates, and so on, are key to understanding nearly any biological process. Mass spectrometry is well established as the method of choice to determine protein primary structure and location of post-translational modifications. In recent years, top-down fragmentation of intact proteins has been increasingly combined with ionisation of noncovalent assemblies under non-denaturing conditions, i.e., native mass spectrometry. Sequence, post-translational modifications, ligand/metal binding, protein folding, and complex stoichiometry can thus all be probed directly. Here, we review recent developments in this new and exciting field of research. While this work is written primarily from a mass spectrometry perspective, it is targeted to all bioanalytical scientists who are interested in applying these methods to their own biochemistry and chemical biology research.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830; FC02-02ER63421

OSTI ID:: 1678783

Alternate ID(s):: OSTI ID: 1755140

Report Number(s):: PNNL-SA-155306; CSHCBM

Journal Information:: Chemical Science, Journal Name: Chemical Science Vol. 11 Journal Issue: 48; ISSN 2041-6520

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (171)

Simple and Minimally Invasive SID Devices for Native Mass Spectrometry Snyder, Dalton T.; Panczyk, Erin M.; Somogyi, Arpad Analytical Chemistry, Vol. 92, Issue 16 https://doi.org/10.1021/acs.analchem.0c01657	journal	July 2020
Recommendations for reporting ion mobility Mass Spectrometry measurements Gabelica, Valérie; Shvartsburg, Alexandre A.; Afonso, Carlos Mass Spectrometry Reviews, Vol. 38, Issue 3 https://doi.org/10.1002/mas.21585	journal	October 2017
Metal Ion Binding to the Amyloid β Monomer Studied by Native Top-Down FTICR Mass Spectrometry Lermyte, Frederik; Everett, James; Lam, Yuko P. Y. Journal of The American Society for Mass Spectrometry, Vol. 30, Issue 10 https://doi.org/10.1007/s13361-019-02283-7	journal	July 2019
Polydispersity of a mammalian chaperone: Mass spectrometry reveals the population of oligomers in B-crystallin Aquilina, J. A.; Benesch, J. L. P.; Bateman, O. A. Proceedings of the National Academy of Sciences, Vol. 100, Issue 19 https://doi.org/10.1073/pnas.1932958100	journal	August 2003
Uncovering the Stoichiometry of Pyrococcus furiosus RNase P, a Multi-Subunit Catalytic Ribonucleoprotein Complex, by Surface-Induced Dissociation and Ion Mobility Mass Spectrometry Ma, Xin; Lai, Lien B.; Lai, Stella M. Angewandte Chemie International Edition, Vol. 53, Issue 43 https://doi.org/10.1002/anie.201405362	journal	September 2014
Topological Analysis of Transthyretin Disassembly Mechanism: Surface-Induced Dissociation Reveals Hidden Reaction Pathways Shirzadeh, Mehdi; Boone, Christopher D.; Laganowsky, Arthur Analytical Chemistry, Vol. 91, Issue 3 https://doi.org/10.1021/acs.analchem.8b05066	journal	January 2019
Elucidating the Tertiary Structure of Protein Ions in Vacuo with Site Specific Photoinitiated Radical Reactions Ly, Tony; Julian, Ryan R. Journal of the American Chemical Society, Vol. 132, Issue 25 https://doi.org/10.1021/ja910665d	journal	June 2010
Gas-phase protein salt bridge stabilities from collisional activation and electron transfer dissociation Zhang, Zhe; Vachet, Richard W. International Journal of Mass Spectrometry, Vol. 420 https://doi.org/10.1016/j.ijms.2016.09.010	journal	September 2017
Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging Uetrecht, Charlotte; Lorenzen, Kristina; Kitel, Matthäus Journal of Synchrotron Radiation, Vol. 26, Issue 3 https://doi.org/10.1107/S1600577519002686	journal	April 2019
Characterization of Native Protein Complexes Using Ultraviolet Photodissociation Mass Spectrometry O’Brien, John P.; Li, Wenzong; Zhang, Yan Journal of the American Chemical Society, Vol. 136, Issue 37 https://doi.org/10.1021/ja505217w	journal	September 2014
Tracking the Catalytic Cycle of Adenylate Kinase by Ultraviolet Photodissociation Mass Spectrometry Mehaffey, M. Rachel; Cammarata, Michael B.; Brodbelt, Jennifer S. Analytical Chemistry, Vol. 90, Issue 1 https://doi.org/10.1021/acs.analchem.7b03591	journal	December 2017
MASH Explorer: A Universal Software Environment for Top-Down Proteomics Wu, Zhijie; Roberts, David S.; Melby, Jake A. Journal of Proteome Research, Vol. 19, Issue 9 https://doi.org/10.1021/acs.jproteome.0c00469	journal	August 2020
Revealing the Quaternary Structure of a Heterogeneous Noncovalent Protein Complex through Surface-Induced Dissociation Blackwell, Anne E.; Dodds, Eric D.; Bandarian, Vahe Analytical Chemistry, Vol. 83, Issue 8 https://doi.org/10.1021/ac200452b	journal	April 2011
Surface-Induced Dissociation Mass Spectra as a Tool for Distinguishing Different Structural Forms of Gas-Phase Multimeric Protein Complexes Quintyn, Royston S.; Zhou, Mowei; Yan, Jing Analytical Chemistry, Vol. 87, Issue 23 https://doi.org/10.1021/acs.analchem.5b03441	journal	November 2015
Mass-spectrometric exploration of proteome structure and function Aebersold, Ruedi; Mann, Matthias Nature, Vol. 537, Issue 7620 https://doi.org/10.1038/nature19949	journal	September 2016
Native mass spectrometry: a bridge between interactomics and structural biology Heck, Albert J. R. Nature Methods, Vol. 5, Issue 11 https://doi.org/10.1038/nmeth.1265	journal	October 2008
Computational Strategies and Challenges for Using Native Ion Mobility Mass Spectrometry in Biophysics and Structural Biology Allison, Timothy M.; Barran, Perdita; Cianférani, Sarah Analytical Chemistry, Vol. 92, Issue 16 https://doi.org/10.1021/acs.analchem.9b05791	journal	July 2020
Facile Disulfide Bond Cleavage in Gaseous Peptide and Protein Cations by Ultraviolet Photodissociation at 157 nm Fung, Y. M. Eva; Kjeldsen, Frank; Silivra, Oleg A. Angewandte Chemie, Vol. 117, Issue 39 https://doi.org/10.1002/ange.200501533	journal	October 2005
Gas-phase conformation-specific photofragmentation of proline-containing peptide ions Kim, Tae-Young; Valentine, Stephen J.; Clemmer, David E. Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 8 https://doi.org/10.1016/j.jasms.2010.04.007	journal	August 2010
Top-down mass spectrometry of supercharged native protein–ligand complexes Yin, Sheng; Loo, Joseph A. International Journal of Mass Spectrometry, Vol. 300, Issue 2-3 https://doi.org/10.1016/j.ijms.2010.06.032	journal	March 2011
Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry Schopper, Simone; Kahraman, Abdullah; Leuenberger, Pascal Nature Protocols, Vol. 12, Issue 11 https://doi.org/10.1038/nprot.2017.100	journal	October 2017
The use of ECD/ETD to identify the site of electrostatic interaction in noncovalent complexes Jackson, Shelley N.; Dutta, Sucharita; Woods, Amina S. Journal of the American Society for Mass Spectrometry, Vol. 20, Issue 2 https://doi.org/10.1016/j.jasms.2008.08.021	journal	February 2009
The molecular sociology of the cell Robinson, Carol V.; Sali, Andrej; Baumeister, Wolfgang Nature, Vol. 450, Issue 7172 https://doi.org/10.1038/nature06523	journal	December 2007
Interpreting raw biological mass spectra using isotopic mass-to-charge ratio and envelope fingerprinting: Interpreting raw biological mass spectra Li, Li; Tian, Zhixin Rapid Communications in Mass Spectrometry, Vol. 27, Issue 11 https://doi.org/10.1002/rcm.6565	journal	May 2013
Exploring Salt Bridge Structures of Gas-Phase Protein Ions using Multiple Stages of Electron Transfer and Collision Induced Dissociation Zhang, Zhe; Browne, Shaynah J.; Vachet, Richard W. Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 4 https://doi.org/10.1007/s13361-013-0821-8	journal	February 2014
Top-down characterization of endogenous protein complexes with native proteomics Skinner, Owen S.; Haverland, Nicole A.; Fornelli, Luca Nature Chemical Biology, Vol. 14, Issue 1 https://doi.org/10.1038/nchembio.2515	journal	November 2017
Krebs Cycle Metabolon: Structural Evidence of Substrate Channeling Revealed by Cross-Linking and Mass Spectrometry Wu, Fei; Minteer, Shelley Angewandte Chemie International Edition, Vol. 54, Issue 6 https://doi.org/10.1002/anie.201409336	journal	December 2014
Surface Induced Dissociation: Dissecting Noncovalent Protein Complexes in the Gas phase Zhou, Mowei; Wysocki, Vicki H. Accounts of Chemical Research, Vol. 47, Issue 4 https://doi.org/10.1021/ar400223t	journal	February 2014
Interaction of angiotensin peptides and zinc metal ions probed by electrospray ionization mass spectrometry Loo, Joseph A.; Hu, Peifeng; Smith, Richard D. Journal of the American Society for Mass Spectrometry, Vol. 5, Issue 11 https://doi.org/10.1016/1044-0305(94)80014-6	journal	November 1994
193 nm Ultraviolet Photodissociation Mass Spectrometry of Tetrameric Protein Complexes Provides Insight into Quaternary and Secondary Protein Topology Morrison, Lindsay J.; Brodbelt, Jennifer S. Journal of the American Chemical Society, Vol. 138, Issue 34 https://doi.org/10.1021/jacs.6b03905	journal	August 2016
From Protein Complexes to Subunit Backbone Fragments: A Multi-stage Approach to Native Mass Spectrometry Belov, Mikhail E.; Damoc, Eugen; Denisov, Eduard Analytical Chemistry, Vol. 85, Issue 23 https://doi.org/10.1021/ac4029328	journal	November 2013
ProSight Lite: Graphical software to analyze top-down mass spectrometry data Fellers, Ryan T.; Greer, Joseph B.; Early, Bryan P. PROTEOMICS, Vol. 15, Issue 7 https://doi.org/10.1002/pmic.201400313	journal	February 2015
Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry as a Platform for Characterizing Multimeric Membrane Protein Complexes Lippens, Jennifer L.; Nshanian, Michael; Spahr, Chris Journal of The American Society for Mass Spectrometry, Vol. 29, Issue 1 https://doi.org/10.1007/s13361-017-1799-4	journal	October 2017
Proteoform: a single term describing protein complexity Smith, Lloyd M.; Kelleher, Neil L. Nature Methods, Vol. 10, Issue 3 https://doi.org/10.1038/nmeth.2369	journal	February 2013
Native Electrospray and Electron-Capture Dissociation FTICR Mass Spectrometry for Top-Down Studies of Protein Assemblies Zhang, Hao; Cui, Weidong; Wen, Jianzhong Analytical Chemistry, Vol. 83, Issue 14 https://doi.org/10.1021/ac200695d	journal	July 2011
Chemical cross-linking with mass spectrometry: a tool for systems structural biology Chavez, Juan D.; Bruce, James E. Current Opinion in Chemical Biology, Vol. 48 https://doi.org/10.1016/j.cbpa.2018.08.006	journal	February 2019
Characterization of Intact Protein Conjugates and Biopharmaceuticals Using Ion-Exchange Chromatography with Online Detection by Native Electrospray Ionization Mass Spectrometry and Top-Down Tandem Mass Spectrometry Muneeruddin, Khaja; Nazzaro, Mark; Kaltashov, Igor A. Analytical Chemistry, Vol. 87, Issue 19 https://doi.org/10.1021/acs.analchem.5b02982	journal	September 2015
Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization Schwartz, Brenda L.; Bruce, James E.; Anderson, Gordon A. Journal of the American Society for Mass Spectrometry, Vol. 6, Issue 6 https://doi.org/10.1016/1044-0305(95)00191-F	journal	June 1995
Mass spectrometry: From plasma proteins to mitochondrial membranes Robinson, Carol V. Proceedings of the National Academy of Sciences, Vol. 116, Issue 8 https://doi.org/10.1073/pnas.1820450116	journal	February 2019
High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles van de Waterbeemd, Michiel; Fort, Kyle L.; Boll, Dmitriy Nature Methods, Vol. 14, Issue 3 https://doi.org/10.1038/nmeth.4147	journal	January 2017
Informed-Proteomics: open-source software package for top-down proteomics Park, Jungkap; Piehowski, Paul D.; Wilkins, Christopher Nature Methods, Vol. 14, Issue 9 https://doi.org/10.1038/nmeth.4388	journal	August 2017
Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications Liu, Xiaoran Roger; Zhang, Mengru Mira; Gross, Michael L. Chemical Reviews, Vol. 120, Issue 10 https://doi.org/10.1021/acs.chemrev.9b00815	journal	April 2020
Initial Protein Unfolding Events in Ubiquitin, Cytochrome c and Myoglobin Are Revealed with the Use of 213 nm UVPD Coupled to IM-MS Theisen, Alina; Black, Rachelle; Corinti, Davide Journal of The American Society for Mass Spectrometry, Vol. 30, Issue 1 https://doi.org/10.1007/s13361-018-1992-0	journal	June 2018
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry Chorev, Dror S.; Baker, Lindsay A.; Wu, Di Science, Vol. 362, Issue 6416 https://doi.org/10.1126/science.aau0976	journal	November 2018
Protein and polymer analyses up tom/z 100 000 by laser ionization time-of-flight mass spectrometry Tanaka, Koichi; Waki, Hiroaki; Ido, Yutaka Rapid Communications in Mass Spectrometry, Vol. 2, Issue 8 https://doi.org/10.1002/rcm.1290020802	journal	August 1988
Charge-State Dependent Compaction and Dissociation of Protein Complexes: Insights from Ion Mobility and Molecular Dynamics Hall, Zoe; Politis, Argyris; Bush, Matthew F. Journal of the American Chemical Society, Vol. 134, Issue 7 https://doi.org/10.1021/ja2096859	journal	February 2012
Degronomics: Mapping the Interacting Peptidome of a Ubiquitin Ligase Using an Integrative Mass Spectrometry Strategy Canzani, Daniele; Rusnac, Domniţa-Valeria; Zheng, Ning Analytical Chemistry, Vol. 91, Issue 20 https://doi.org/10.1021/acs.analchem.9b02331	journal	September 2019
Native Top-Down Mass Spectrometry and Ion Mobility Spectrometry of the Interaction of Tau Protein with a Molecular Tweezer Assembly Modulator Nshanian, Michael; Lantz, Carter; Wongkongkathep, Piriya Journal of The American Society for Mass Spectrometry, Vol. 30, Issue 1 https://doi.org/10.1007/s13361-018-2027-6	journal	July 2018
Localization of Protein Complex Bound Ligands by Surface-Induced Dissociation High-Resolution Mass Spectrometry Busch, Florian; VanAernum, Zachary L.; Ju, Yue Analytical Chemistry, Vol. 90, Issue 21 https://doi.org/10.1021/acs.analchem.8b03263	journal	October 2018
Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process Zubarev, Roman A.; Kelleher, Neil L.; McLafferty, Fred W. Journal of the American Chemical Society, Vol. 120, Issue 13 https://doi.org/10.1021/ja973478k	journal	April 1998
Collision induced unfolding of isolated proteins in the gas phase: past, present, and future Dixit, Sugyan M.; Polasky, Daniel A.; Ruotolo, Brandon T. Current Opinion in Chemical Biology, Vol. 42 https://doi.org/10.1016/j.cbpa.2017.11.010	journal	February 2018
Collision-Induced Dissociation of Electrosprayed Protein Complexes: An All-Atom Molecular Dynamics Model with Mobile Protons Popa, Vlad; Trecroce, Danielle A.; McAllister, Robert G. The Journal of Physical Chemistry B, Vol. 120, Issue 23 https://doi.org/10.1021/acs.jpcb.6b03035	journal	May 2016
Shattering of Peptide Ions on Self-Assembled Monolayer Surfaces Laskin, Julia; Bailey, Thomas H.; Futrell, Jean H. Journal of the American Chemical Society, Vol. 125, Issue 6 https://doi.org/10.1021/ja027915t	journal	February 2003
Mass-resolved electronic circular dichroism ion spectroscopy Daly, Steven; Rosu, Frédéric; Gabelica, Valérie Science, Vol. 368, Issue 6498 https://doi.org/10.1126/science.abb1822	journal	June 2020
Analytical Properties of the Nanoelectrospray Ion Source Wilm, Matthias; Mann, Matthias Analytical Chemistry, Vol. 68, Issue 1 https://doi.org/10.1021/ac9509519	journal	January 1996
Top-Down Proteomics: Ready for Prime Time? Chen, Bifan; Brown, Kyle A.; Lin, Ziqing Analytical Chemistry, Vol. 90, Issue 1 https://doi.org/10.1021/acs.analchem.7b04747	journal	November 2017
Programmable design of orthogonal protein heterodimers Chen, Zibo; Boyken, Scott E.; Jia, Mengxuan Nature, Vol. 565, Issue 7737 https://doi.org/10.1038/s41586-018-0802-y	journal	December 2018
Description and control of dissociation channels in gas-phase protein complexes Thachuk, Mark; Fegan, Sarah K.; Raheem, Nigare The Journal of Chemical Physics, Vol. 145, Issue 6 https://doi.org/10.1063/1.4960615	journal	August 2016
Surface Induced Dissociation Yields Quaternary Substructure of Refractory Noncovalent Phosphorylase B and Glutamate Dehydrogenase Complexes Ma, Xin; Zhou, Mowei; Wysocki, Vicki H. Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 3 https://doi.org/10.1007/s13361-013-0790-y	journal	January 2014
A molecular mechanism for transthyretin amyloidogenesis Yee, Ai Woon; Aldeghi, Matteo; Blakeley, Matthew P. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-08609-z	journal	February 2019
Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes Kafader, Jared O.; Melani, Rafael D.; Durbin, Kenneth R. Nature Methods, Vol. 17, Issue 4 https://doi.org/10.1038/s41592-020-0764-5	journal	March 2020
Surface-Induced Dissociation of Homotetramers with D2 Symmetry Yields their Assembly Pathways and Characterizes the Effect of Ligand Binding Quintyn, Royston S.; Yan, Jing; Wysocki, Vicki H. Chemistry & Biology, Vol. 22, Issue 5 https://doi.org/10.1016/j.chembiol.2015.03.019	journal	May 2015
Determination of the Aggregate Binding Site of Amyloid Protofibrils Using Electron Capture Dissociation Tandem Mass Spectrometry Lam, Yuko P. Y.; Wootton, Christopher A.; Hands-Portman, Ian Journal of the American Society for Mass Spectrometry, Vol. 31, Issue 2 https://doi.org/10.1021/jasms.9b00053	journal	January 2020
Origin of Asymmetric Charge Partitioning in the Dissociation of Gas-Phase Protein Homodimers Jurchen, John C.; Williams, Evan R. Journal of the American Chemical Society, Vol. 125, Issue 9 https://doi.org/10.1021/ja0211508	journal	March 2003
Mass spectrometry-based proteomics Aebersold, Ruedi; Mann, Matthias Nature, Vol. 422, Issue 6928 https://doi.org/10.1038/nature01511	journal	March 2003
Differences in the Elemental Isotope Definition May Lead to Errors in Modern Mass-Spectrometry-Based Proteomics Claesen, Jürgen; Lermyte, Frederik; Sobott, Frank Analytical Chemistry, Vol. 87, Issue 21 https://doi.org/10.1021/acs.analchem.5b01165	journal	July 2015
Relative interfacial cleavage energetics of protein complexes revealed by surface collisions Harvey, Sophie R.; Seffernick, Justin T.; Quintyn, Royston S. Proceedings of the National Academy of Sciences, Vol. 116, Issue 17 https://doi.org/10.1073/pnas.1817632116	journal	April 2019
Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex Zhou, Mowei; Yan, Jing; Romano, Christine A. Journal of The American Society for Mass Spectrometry, Vol. 29, Issue 4 https://doi.org/10.1007/s13361-017-1882-x	journal	January 2018
Revealing Ligand Binding Sites and Quantifying Subunit Variants of Noncovalent Protein Complexes in a Single Native Top-Down FTICR MS Experiment Li, Huilin; Wongkongkathep, Piriya; Van Orden, Steve L. Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 12 https://doi.org/10.1007/s13361-014-0928-6	journal	June 2014
Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase Ro, Soo Y.; Schachner, Luis F.; Koo, Christopher W. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-10590-6	journal	June 2019
Direct Determination of Antibody Chain Pairing by Top-down and Middle-down Mass Spectrometry Using Electron Capture Dissociation and Ultraviolet Photodissociation Shaw, Jared B.; Liu, Weijing; Vasil′ev, Yury V. Analytical Chemistry, Vol. 92, Issue 1 https://doi.org/10.1021/acs.analchem.9b03129	journal	November 2019
Ion mobility in the pharmaceutical industry: an established biophysical technique or still niche? Campuzano, Iain DG; Lippens, Jennifer L. Current Opinion in Chemical Biology, Vol. 42 https://doi.org/10.1016/j.cbpa.2017.11.008	journal	February 2018
Electrospray ionization for mass spectrometry of large biomolecules Fenn, J.; Mann, M.; Meng, C. Science, Vol. 246, Issue 4926, p. 64-71 https://doi.org/10.1126/science.2675315	journal	October 1989
Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria Ilag, L. L.; Videler, H.; McKay, A. R. Proceedings of the National Academy of Sciences, Vol. 102, Issue 23 https://doi.org/10.1073/pnas.0502193102	journal	May 2005
Confirmation of intersubunit connectivity and topology of designed protein complexes by native MS Sahasrabuddhe, Aniruddha; Hsia, Yang; Busch, Florian Proceedings of the National Academy of Sciences, Vol. 115, Issue 6 https://doi.org/10.1073/pnas.1713646115	journal	January 2018
Cross-Linking/Mass Spectrometry for Studying Protein Structures and Protein-Protein Interactions: Where Are We Now and Where Should We Go from Here? Sinz, Andrea Angewandte Chemie International Edition, Vol. 57, Issue 22 https://doi.org/10.1002/anie.201709559	journal	April 2018
Stepwise evolution of protein native structure with electrospray into the gas phase, 10-12 to 102 s Breuker, K.; McLafferty, F. W. Proceedings of the National Academy of Sciences, Vol. 105, Issue 47 https://doi.org/10.1073/pnas.0807005105	journal	November 2008
Salt Bridge Rearrangement (SaBRe) Explains the Dissociation Behavior of Noncovalent Complexes Loo, Rachel R. Ogorzalek; Loo, Joseph A. Journal of The American Society for Mass Spectrometry, Vol. 27, Issue 6 https://doi.org/10.1007/s13361-016-1375-3	journal	April 2016
MASH Suite Pro: A Comprehensive Software Tool for Top-Down Proteomics Cai, Wenxuan; Guner, Huseyin; Gregorich, Zachery R. Molecular & Cellular Proteomics, Vol. 15, Issue 2 https://doi.org/10.1074/mcp.O115.054387	journal	November 2015
Specific sequences in the N-terminal domain of human small heat-shock protein HSPB6 dictate preferential hetero-oligomerization with the orthologue HSPB1 Heirbaut, Michelle; Lermyte, Frederik; Martin, Esther M. Journal of Biological Chemistry, Vol. 292, Issue 24 https://doi.org/10.1074/jbc.M116.773515	journal	May 2017
Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry Zheng, Jie; Strutzenberg, Timothy; Pascal, Bruce D. Current Opinion in Structural Biology, Vol. 58 https://doi.org/10.1016/j.sbi.2019.06.007	journal	October 2019
Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein Konijnenberg, Albert; Ranica, Simona; Narkiewicz, Joanna Analytical Chemistry, Vol. 88, Issue 17 https://doi.org/10.1021/acs.analchem.6b00731	journal	August 2016
Small Emitter Tips for Native Mass Spectrometry of Proteins and Protein Complexes from Nonvolatile Buffers That Mimic the Intracellular Environment Susa, Anna C.; Xia, Zijie; Williams, Evan R. Analytical Chemistry, Vol. 89, Issue 5 https://doi.org/10.1021/acs.analchem.6b04897	journal	February 2017
Charge-Induced Unzipping of Isolated Proteins to a Defined Secondary Structure González Flórez, Ana Isabel; Mucha, Eike; Ahn, Doo-Sik Angewandte Chemie International Edition, Vol. 55, Issue 10 https://doi.org/10.1002/anie.201510983	journal	February 2016
Native Mass Spectrometry: What is in the Name? Leney, Aneika C.; Heck, Albert J. R. Journal of The American Society for Mass Spectrometry, Vol. 28, Issue 1 https://doi.org/10.1007/s13361-016-1545-3	journal	December 2016
Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells Fan, Yanlin; Guo, Yusong R.; Yuan, Wang PLOS Pathogens, Vol. 13, Issue 2 https://doi.org/10.1371/journal.ppat.1006231	journal	February 2017
The Mechanism Behind Top-Down UVPD Experiments: Making Sense of Apparent Contradictions R. Julian, Ryan Journal of The American Society for Mass Spectrometry, Vol. 28, Issue 9 https://doi.org/10.1007/s13361-017-1721-0	journal	July 2017
MASH Suite: A User-Friendly and Versatile Software Interface for High-Resolution Mass Spectrometry Data Interpretation and Visualization Guner, Huseyin; Close, Patrick L.; Cai, Wenxuan Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 3 https://doi.org/10.1007/s13361-013-0789-4	journal	January 2014
Structural Evaluation of Protein/Metal Complexes via Native Electrospray Ultraviolet Photodissociation Mass Spectrometry Crittenden, Christopher M.; Novelli, Elisa T.; Mehaffey, M. Rachel Journal of the American Society for Mass Spectrometry, Vol. 31, Issue 5 https://doi.org/10.1021/jasms.0c00066	journal	April 2020
Experimental and theoretical investigation of overall energy deposition in surface-induced unfolding of protein ions Donor, Micah T.; Mroz, Austin M.; Prell, James S. Chemical Science, Vol. 10, Issue 14 https://doi.org/10.1039/C9SC00644C	journal	January 2019
Noncovalent Protein Tetramers and Pentamers with “ n ” Charges Yield Monomers with n /4 and n /5 Charges Beardsley, Richard L.; Jones, Christopher M.; Galhena, Asiri S. Analytical Chemistry, Vol. 81, Issue 4 https://doi.org/10.1021/ac801883k	journal	February 2009
Imaging proteins at the single-molecule level Longchamp, Jean-Nicolas; Rauschenbach, Stephan; Abb, Sabine Proceedings of the National Academy of Sciences, Vol. 114, Issue 7 https://doi.org/10.1073/pnas.1614519114	journal	January 2017
Top or Middle? Up or Down? Toward a Standard Lexicon for Protein Top-Down and Allied Mass Spectrometry Approaches Lermyte, Frederik; Tsybin, Yury O.; O’Connor, Peter B. Journal of The American Society for Mass Spectrometry, Vol. 30, Issue 7 https://doi.org/10.1007/s13361-019-02201-x	journal	May 2019
Electron capture dissociation and drift tube ion mobility-mass spectrometry coupled with site directed mutations provide insights into the conformational diversity of a metamorphic protein Harvey, Sophie R.; Porrini, Massimiliano; Tyler, Robert C. Physical Chemistry Chemical Physics, Vol. 17, Issue 16 https://doi.org/10.1039/C4CP05136J	journal	January 2015
Native Top-down Mass Spectrometry for the Structural Characterization of Human Hemoglobin Zhang, Jiang; Malmirchegini, G. Reza; Clubb, Robert T. European Journal of Mass Spectrometry, Vol. 21, Issue 3 https://doi.org/10.1255/ejms.1340	journal	June 2015
Native Proteomics in Discovery Mode Using Size-Exclusion Chromatography–Capillary Zone Electrophoresis–Tandem Mass Spectrometry Shen, Xiaojing; Kou, Qiang; Guo, Ruiqiong Analytical Chemistry, Vol. 90, Issue 17 https://doi.org/10.1021/acs.analchem.8b02725	journal	July 2018
Dissecting the Dynamic Conformations of the Metamorphic Protein Lymphotactin Harvey, Sophie R.; Porrini, Massimiliano; Konijnenberg, Albert The Journal of Physical Chemistry B, Vol. 118, Issue 43 https://doi.org/10.1021/jp504997k	journal	October 2014
Photodissociation mass spectrometry: new tools for characterization of biological molecules Brodbelt, Jennifer S. Chem. Soc. Rev., Vol. 43, Issue 8 https://doi.org/10.1039/C3CS60444F	journal	January 2014
`masstodon` : A Tool for Assigning Peaks and Modeling Electron Transfer Reactions in Top-Down Mass Spectrometry Łącki, Mateusz K.; Lermyte, Frederik; Miasojedow, Błażej Analytical Chemistry, Vol. 91, Issue 3 https://doi.org/10.1021/acs.analchem.8b01479	journal	January 2019
Mapping a Noncovalent Protein–Peptide Interface by Top-Down FTICR Mass Spectrometry Using Electron Capture Dissociation Clarke, David J.; Murray, Euan; Hupp, Ted Journal of The American Society for Mass Spectrometry, Vol. 22, Issue 8 https://doi.org/10.1007/s13361-011-0155-3	journal	May 2011
Retention of Native Protein Structures in the Absence of Solvent: A Coupled Ion Mobility and Spectroscopic Study Seo, Jongcheol; Hoffmann, Waldemar; Warnke, Stephan Angewandte Chemie International Edition, Vol. 55, Issue 45 https://doi.org/10.1002/anie.201606029	journal	August 2016
TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization Kou, Qiang; Xun, Likun; Liu, Xiaowen Bioinformatics https://doi.org/10.1093/bioinformatics/btw398	journal	July 2016
Dissection of multi-protein complexes using mass spectrometry: Subunit interactions in transthyretin and retinol-binding protein complexes Rostom, Adam A.; Sunde, Margaret; Richardson, Samantha J. Proteins: Structure, Function, and Genetics, Vol. 33, Issue S2 https://doi.org/10.1002/(SICI)1097-0134(1998)33:2+<3::AID-PROT2>3.0.CO;2-H	journal	January 1998
Observation of the noncovalent assembly and disassembly pathways of the chaperone complex MtGimC by mass spectrometry Fandrich, M.; Tito, M. A.; Leroux, M. R. Proceedings of the National Academy of Sciences, Vol. 97, Issue 26 https://doi.org/10.1073/pnas.240326597	journal	November 2000
Impact of charge state on gas-phase behaviors of noncovalent protein complexes in collision induced dissociation and surface induced dissociation Zhou, Mowei; Dagan, Shai; Wysocki, Vicki H. The Analyst, Vol. 138, Issue 5 https://doi.org/10.1039/c2an36525a	journal	January 2013
Hydrogen-Deuterium Exchange Coupled to Top- and Middle-Down Mass Spectrometry Reveals Histone Tail Dynamics before and after Nucleosome Assembly Karch, Kelly R.; Coradin, Mariel; Zandarashvili, Levani Structure, Vol. 26, Issue 12 https://doi.org/10.1016/j.str.2018.08.006	journal	December 2018
Increasing fragmentation of disulfide-bonded proteins for top–down mass spectrometry by supercharging Zhang, Jiang; Loo, Rachel R. Ogorzalek; Loo, Joseph A. International Journal of Mass Spectrometry, Vol. 377 https://doi.org/10.1016/j.ijms.2014.07.047	journal	February 2015
Native Top-Down Mass Spectrometry of TAR RNA in Complexes with a Wild-Type tat Peptide for Binding Site Mapping Schneeberger, Eva-Maria; Breuker, Kathrin Angewandte Chemie, Vol. 129, Issue 5 https://doi.org/10.1002/ange.201610836	journal	December 2016
Detailed Unfolding and Folding of Gaseous Ubiquitin Ions Characterized by Electron Capture Dissociation Breuker, Kathrin; Oh, HanBin; Horn, David M. Journal of the American Chemical Society, Vol. 124, Issue 22 https://doi.org/10.1021/ja012267j	journal	June 2002
PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry Takemori, Ayako; Butcher, David S.; Harman, Victoria M. Journal of Proteome Research, Vol. 19, Issue 9 https://doi.org/10.1021/acs.jproteome.0c00303	journal	June 2020
ETD Allows for Native Surface Mapping of a 150 kDa Noncovalent Complex on a Commercial Q-TWIMS-TOF Instrument Lermyte, Frederik; Konijnenberg, Albert; Williams, Jonathan P. Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 3 https://doi.org/10.1007/s13361-013-0798-3	journal	January 2014
A Tandem Mass Spectrometer for Improved Transmission and Analysis of Large Macromolecular Assemblies Sobott, Frank; Hernández, Helena; McCammon, Margaret G. Analytical Chemistry, Vol. 74, Issue 6, p. 1402-1407 https://doi.org/10.1021/ac0110552	journal	March 2002
Impact of charge state on 193 nm ultraviolet photodissociation of protein complexes Sipe, Sarah N.; Brodbelt, Jennifer S. Physical Chemistry Chemical Physics, Vol. 21, Issue 18 https://doi.org/10.1039/C9CP01144G	journal	January 2019
213 nm Ultraviolet Photodissociation on Peptide Anions: Radical-Directed Fragmentation Patterns Halim, Mohammad A.; Girod, Marion; MacAleese, Luke Journal of The American Society for Mass Spectrometry, Vol. 27, Issue 3 https://doi.org/10.1007/s13361-015-1297-5	journal	November 2015
Identification and Quantification of Proteoforms by Mass Spectrometry Schaffer, Leah V.; Millikin, Robert J.; Miller, Rachel M. PROTEOMICS, Vol. 19, Issue 10 https://doi.org/10.1002/pmic.201800361	journal	May 2019
Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins Gault, Joseph; Liko, Idlir; Landreh, Michael Nature Methods, Vol. 17, Issue 5 https://doi.org/10.1038/s41592-020-0821-0	journal	May 2020
Top-Down ESI-ECD-FT-ICR Mass Spectrometry Localizes Noncovalent Protein-Ligand Binding Sites Xie, Yongming; Zhang, Jennifer; Yin, Sheng Journal of the American Chemical Society, Vol. 128, Issue 45 https://doi.org/10.1021/ja063197p	journal	November 2006
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry Syka, J. E. P.; Coon, J. J.; Schroeder, M. J. Proceedings of the National Academy of Sciences, Vol. 101, Issue 26 https://doi.org/10.1073/pnas.0402700101	journal	June 2004
Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry Vimer, Shay; Ben-Nissan, Gili; Morgenstern, David ACS Central Science, Vol. 6, Issue 4 https://doi.org/10.1021/acscentsci.0c00080	journal	April 2020
Observation of the Noncovalent Quaternary Associations of Proteins by Electrospray Ionization Mass Spectrometry Light-Wahl, Karen J.; Schwartz, Brenda L.; Smith, Richard D. Journal of the American Chemical Society, Vol. 116, Issue 12 https://doi.org/10.1021/ja00091a035	journal	June 1994
Online Hydrophobic Interaction Chromatography–Mass Spectrometry for the Analysis of Intact Monoclonal Antibodies Chen, Bifan; Lin, Ziqing; Alpert, Andrew J. Analytical Chemistry, Vol. 90, Issue 12 https://doi.org/10.1021/acs.analchem.8b01865	journal	May 2018
Evolution of Structural Biology through the Lens of Mass Spectrometry Kaur, Upneet; Johnson, Danté T.; Chea, Emily E. Analytical Chemistry, Vol. 91, Issue 1 https://doi.org/10.1021/acs.analchem.8b05014	journal	November 2018
Mapping Proteoforms and Protein Complexes From King Cobra Venom Using Both Denaturing and Native Top-down Proteomics Melani, Rafael D.; Skinner, Owen S.; Fornelli, Luca Molecular & Cellular Proteomics, Vol. 15, Issue 7 https://doi.org/10.1074/mcp.M115.056523	journal	May 2016
Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry Zhou, Mowei; Liu, Weijing; Shaw, Jared B. Analytical Chemistry, Vol. 92, Issue 2 https://doi.org/10.1021/acs.analchem.9b03469	journal	December 2019
Thermal Decomposition of a Gaseous Multiprotein Complex Studied by Blackbody Infrared Radiative Dissociation. Investigating the Origin of the Asymmetric Dissociation Behavior Felitsyn, Natalia; Kitova, Elena N.; Klassen, John S. Analytical Chemistry, Vol. 73, Issue 19 https://doi.org/10.1021/ac0103975	journal	October 2001
Structural Characterization of Native Proteins and Protein Complexes by Electron Ionization Dissociation-Mass Spectrometry Li, Huilin; Sheng, Yuewei; McGee, William Analytical Chemistry, Vol. 89, Issue 5 https://doi.org/10.1021/acs.analchem.6b02377	journal	February 2017
Kinetic Intermediates in the Folding of Gaseous Protein Ions Characterized by Electron Capture Dissociation Mass Spectrometry Horn, David M.; Breuker, Kathrin; Frank, Aaron J. Journal of the American Chemical Society, Vol. 123, Issue 40 https://doi.org/10.1021/ja003143u	journal	October 2001
Soft X-ray Spectroscopy as a Probe for Gas-Phase Protein Structure: Electron Impact Ionization from Within Bari, Sadia; Egorov, Dmitrii; Jansen, Thomas L. C. Chemistry - A European Journal, Vol. 24, Issue 30 https://doi.org/10.1002/chem.201801440	journal	May 2018
ProSight PTM 2.0: improved protein identification and characterization for top down mass spectrometry Zamdborg, L.; LeDuc, R. D.; Glowacz, K. J. Nucleic Acids Research, Vol. 35, Issue Web Server https://doi.org/10.1093/nar/gkm371	journal	May 2007
Electron transfer dissociation provides higher-order structural information of native and partially unfolded protein complexes Lermyte, Frederik; Sobott, Frank PROTEOMICS, Vol. 15, Issue 16 https://doi.org/10.1002/pmic.201400516	journal	July 2015
Identifying drug metallation sites on peptides using electron transfer dissociation (ETD), collision induced dissociation (CID) and ion mobility-mass spectrometry (IM-MS) Williams, Jonathan P.; Brown, Jeffery M.; Campuzano, Iain Chemical Communications, Vol. 46, Issue 30 https://doi.org/10.1039/c0cc00358a	journal	January 2010
Structure of the Yeast Vacuolar ATPase Zhang, Zhenyu; Zheng, Yesha; Mazon, Hortense Journal of Biological Chemistry, Vol. 283, Issue 51 https://doi.org/10.1074/jbc.M805345200	journal	October 2008
Native Top-Down Mass Spectrometry and Ion Mobility MS for Characterizing the Cobalt and Manganese Metal Binding of α-Synuclein Protein Wongkongkathep, Piriya; Han, Jong Yoon; Choi, Tae Su Journal of The American Society for Mass Spectrometry, Vol. 29, Issue 9 https://doi.org/10.1007/s13361-018-2002-2	journal	June 2018
The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics Lanucara, Francesco; Holman, Stephen W.; Gray, Christopher J. Nature Chemistry, Vol. 6, Issue 4 https://doi.org/10.1038/nchem.1889	journal	March 2014
Online Hydrophobic Interaction Chromatography–Mass Spectrometry for Top-Down Proteomics Chen, Bifan; Peng, Ying; Valeja, Santosh G. Analytical Chemistry, Vol. 88, Issue 3 https://doi.org/10.1021/acs.analchem.5b04285	journal	January 2016
Protein Footprinting Comes of Age: Mass Spectrometry for Biophysical Structure Assessment Wang, Liwen; Chance, Mark R. Molecular & Cellular Proteomics, Vol. 16, Issue 5 https://doi.org/10.1074/mcp.O116.064386	journal	March 2017
Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry Cammarata, Michael B.; Brodbelt, Jennifer S. Chemical Science, Vol. 6, Issue 2 https://doi.org/10.1039/C4SC03200D	journal	January 2015
Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx Romano, Christine A.; Zhou, Mowei; Song, Yang Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00896-8	journal	September 2017
Top-Down Characterization of Denatured Proteins and Native Protein Complexes Using Electron Capture Dissociation Implemented within a Modified Ion Mobility-Mass Spectrometer Williams, Jonathan P.; Morrison, Lindsay J.; Brown, Jeffery M. Analytical Chemistry, Vol. 92, Issue 5 https://doi.org/10.1021/acs.analchem.9b04763	journal	January 2020
Electron Capture Dissociation as Structural Probe for Noncovalent Gas-Phase Protein Assemblies Geels, Rimco B. J.; van der Vies, Saskia M.; Heck, Albert J. R. Analytical Chemistry, Vol. 78, Issue 20 https://doi.org/10.1021/ac060960p	journal	October 2006
Alternate Dissociation Pathways Identified in Charge-Reduced Protein Complex Ions Pagel, Kevin; Hyung, Suk-Joon; Ruotolo, Brandon T. Analytical Chemistry, Vol. 82, Issue 12 https://doi.org/10.1021/ac101121r	journal	June 2010
An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes Li, Huilin; Nguyen, Hong Hanh; Ogorzalek Loo, Rachel R. Nature Chemistry, Vol. 10, Issue 2 https://doi.org/10.1038/nchem.2908	journal	January 2018
Gas-Phase Coordination Properties of Zn2+, Cu2+, Ni2+, and Co2+ with Histidine-Containing Peptides Hu, Peifeng; Loo, Joseph A. Journal of the American Chemical Society, Vol. 117, Issue 45 https://doi.org/10.1021/ja00150a032	journal	November 1995
Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments Masson, Glenn R.; Burke, John E.; Ahn, Natalie G. Nature Methods, Vol. 16, Issue 7 https://doi.org/10.1038/s41592-019-0459-y	journal	June 2019
Surface-Induced Dissociation of Peptides and Protein Complexes in a Quadrupole/Time-of-Flight Mass Spectrometer Galhena, Asiri S.; Dagan, Shai; Jones, Christopher M. Analytical Chemistry, Vol. 80, Issue 5 https://doi.org/10.1021/ac701782q	journal	March 2008
Charge site assignment in native proteins by ultraviolet photodissociation (UVPD) mass spectrometry Morrison, Lindsay J.; Brodbelt, Jennifer S. The Analyst, Vol. 141, Issue 1 https://doi.org/10.1039/C5AN01819F	journal	January 2016
Internal Fragments Generated by Electron Ionization Dissociation Enhance Protein Top-Down Mass Spectrometry Zenaidee, Muhammad A.; Lantz, Carter; Perkins, Taylor Journal of the American Society for Mass Spectrometry, Vol. 31, Issue 9 https://doi.org/10.1021/jasms.0c00160	journal	August 2020
Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure Lermyte, Frederik; Valkenborg, Dirk; Loo, Joseph A. Mass Spectrometry Reviews, Vol. 37, Issue 6 https://doi.org/10.1002/mas.21560	journal	February 2018
Unique insights to intrinsically disordered proteins provided by ion mobility mass spectrometry Stuchfield, Dale; Barran, Perdita Current Opinion in Chemical Biology, Vol. 42 https://doi.org/10.1016/j.cbpa.2018.01.007	journal	February 2018
Simulations of buckminsterfullerene (C60) collisions with a hydrogen-terminated diamond {111} surface Mowrey, R. C.; Brenner, D. W.; Dunlap, B. I. The Journal of Physical Chemistry, Vol. 95, Issue 19 https://doi.org/10.1021/j100172a011	journal	September 1991
Fragmentation of Integral Membrane Proteins in the Gas Phase Skinner, Owen S.; Catherman, Adam D.; Early, Bryan P. Analytical Chemistry, Vol. 86, Issue 9 https://doi.org/10.1021/ac500864w	journal	April 2014
Top-down mass spectrometry of intact membrane protein complexes reveals oligomeric state and sequence information in a single experiment: Top-down MS of Membrane Proteins Konijnenberg, Albert; Bannwarth, Ludovic; Yilmaz, Duygu Protein Science, Vol. 24, Issue 8 https://doi.org/10.1002/pro.2703	journal	May 2015
Molecular Dynamics Simulations on Gas-Phase Proteins with Mobile Protons: Inclusion of All-Atom Charge Solvation Konermann, Lars The Journal of Physical Chemistry B, Vol. 121, Issue 34 https://doi.org/10.1021/acs.jpcb.7b05703	journal	August 2017
Mass spectrometry of intact membrane protein complexes Laganowsky, Arthur; Reading, Eamonn; Hopper, Jonathan T. S. Nature Protocols, Vol. 8, Issue 4 https://doi.org/10.1038/nprot.2013.024	journal	March 2013
Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry Wörner, Tobias P.; Snijder, Joost; Bennett, Antonette Nature Methods, Vol. 17, Issue 4 https://doi.org/10.1038/s41592-020-0770-7	journal	March 2020
Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry Sipe, Sarah N.; Patrick, John W.; Laganowsky, Arthur Analytical Chemistry, Vol. 92, Issue 1 https://doi.org/10.1021/acs.analchem.9b03689	journal	November 2019
Mass-Selective Soft-Landing of Protein Assemblies with Controlled Landing Energies Mikhailov, Victor A.; Mize, Todd H.; Benesch, Justin L. P. Analytical Chemistry, Vol. 86, Issue 16 https://doi.org/10.1021/ac5018327	journal	July 2014
Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry VanAernum, Zachary L.; Busch, Florian; Jones, Benjamin J. Nature Protocols, Vol. 15, Issue 3 https://doi.org/10.1038/s41596-019-0281-0	journal	January 2020
Proteoforms as the next proteomics currency Smith, Lloyd M.; Kelleher, Neil L. Science, Vol. 359, Issue 6380 https://doi.org/10.1126/science.aat1884	journal	March 2018
Refining the Structural Model of a Heterohexameric Protein Complex: Surface Induced Dissociation and Ion Mobility Provide Key Connectivity and Topology Information Song, Yang; Nelp, Micah T.; Bandarian, Vahe ACS Central Science, Vol. 1, Issue 9 https://doi.org/10.1021/acscentsci.5b00251	journal	November 2015
Surface-Induced Dissociation: An Effective Method for Characterization of Protein Quaternary Structure Stiving, Alyssa Q.; VanAernum, Zachary L.; Busch, Florian Analytical Chemistry, Vol. 91, Issue 1 https://doi.org/10.1021/acs.analchem.8b05071	journal	November 2018
Statistical Examination of the a and a + 1 Fragment Ions from 193 nm Ultraviolet Photodissociation Reveals Local Hydrogen Bonding Interactions Morrison, Lindsay J.; Rosenberg, Jake A.; Singleton, Jonathan P. Journal of The American Society for Mass Spectrometry, Vol. 27, Issue 9 https://doi.org/10.1007/s13361-016-1418-9	journal	May 2016
How many human proteoforms are there? Aebersold, Ruedi; Agar, Jeffrey N.; Amster, I. Jonathan Nature Chemical Biology, Vol. 14, Issue 3 https://doi.org/10.1038/nchembio.2576	journal	February 2018
Electron-capture dissociation and ion mobility mass spectrometry for characterization of the hemoglobin protein assembly: ECD & IM for Hemoglobin Cui, Weidong; Zhang, Hao; Blankenship, Robert E. Protein Science, Vol. 24, Issue 8 https://doi.org/10.1002/pro.2712	journal	July 2015
Structural Characterization of Dihydrofolate Reductase Complexes by Top-Down Ultraviolet Photodissociation Mass Spectrometry Cammarata, Michael B.; Thyer, Ross; Rosenberg, Jake Journal of the American Chemical Society, Vol. 137, Issue 28 https://doi.org/10.1021/jacs.5b04628	journal	July 2015
Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state Snijder, Joost; Schuller, Jan M.; Wiegard, Anika Science, Vol. 355, Issue 6330 https://doi.org/10.1126/science.aag3218	journal	March 2017
Characterization of hydrogen bonding motifs in proteins: hydrogen elimination monitoring by ultraviolet photodissociation mass spectrometry Morrison, Lindsay J.; Chai, Wenrui; Rosenberg, Jake A. Physical Chemistry Chemical Physics, Vol. 19, Issue 30 https://doi.org/10.1039/C7CP04073C	journal	January 2017
UV Photodissociation of Proline-containing Peptide Ions: Insights from Molecular Dynamics Girod, Marion; Sanader, Zeljka; Vojkovic, Marin Journal of The American Society for Mass Spectrometry, Vol. 26, Issue 3 https://doi.org/10.1007/s13361-014-1038-1	journal	December 2014
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons Karas, Michael.; Hillenkamp, Franz. Analytical Chemistry, Vol. 60, Issue 20 https://doi.org/10.1021/ac00171a028	journal	October 1988
Triple-Stage Mass Spectrometry Unravels the Heterogeneity of an Endogenous Protein Complex Ben-Nissan, Gili; Belov, Mikhail E.; Morgenstern, David Analytical Chemistry, Vol. 89, Issue 8 https://doi.org/10.1021/acs.analchem.7b00518	journal	April 2017
Bayesian Deconvolution of Mass and Ion Mobility Spectra: From Binary Interactions to Polydisperse Ensembles Marty, Michael T.; Baldwin, Andrew J.; Marklund, Erik G. Analytical Chemistry, Vol. 87, Issue 8 https://doi.org/10.1021/acs.analchem.5b00140	journal	April 2015

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