Chapter 2: Genetics of Unstudied Thermophiles for Industry

Chung, Daehwan; Sarai, Nicholas S.; Himmel, Michael E.; Bomble, Yannick J.

doi:10.1007/978-1-0716-0195-2_2

Chapter 2: Genetics of Unstudied Thermophiles for Industry

Book · Tue Jul 28 04:00:00 EDT 2020

DOI:https://doi.org/10.1007/978-1-0716-0195-2_2· OSTI ID:1677458

Chung, Daehwan; Sarai, Nicholas S.; Himmel, Michael E.; Bomble, Yannick J.

Thermophilic organisms hold great potential for industry due to their numerous advantages in biotechnological applications such as higher reaction rate, higher substrate loading, decreased susceptibility to reaction contamination, energy savings in industrial fermentations, and ability to express thermostable proteins that can be utilized in many important industrial processes. Bioprospecting for thermophiles will continue to reveal new enzymatic and metabolic paradigms with industrial applicability. In order to translate these paradigms to production scale, routine methods for microbial genetic engineering are needed, yet remain to be developed in many newly isolated thermophiles. Major challenges and recent developments in the establishment of reliable genetic systems in thermophiles are discussed. Here, we use a hyperthermophilic, cellulolytic bacterium, Caldicellulosiruptor bescii, as a case study to demonstrate the development of a genetic system for an industrially useful thermophile, describing in detail methods for transformation, genetic tool utilization, and chromosomal modification using targeted gene deletion and insertion techniques.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 1677458

Report Number(s):: NREL/CH-2700-74950; MainId:6671; UUID:9cbfb881-0bdb-e911-9c26-ac162d87dfe5; MainAdminID:18648

Country of Publication:: United States

Language:: English

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Chapter 2: Genetics of Unstudied Thermophiles for Industry

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