Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes

Loftus, Sarah E.; Hunt, Dana E.; Johnson, Zackary I.

doi:10.1016/j.algal.2020.102067

Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes

Journal Article · Fri Sep 18 00:00:00 EDT 2020 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2020.102067· OSTI ID:1673383

Loftus, Sarah E. ^[1]; Hunt, Dana E. ^[2]; ^[2]

Duke Univ., Durham, NC (United States); Duke University
Duke Univ., Durham, NC (United States)

Economical production of algal commodities (food, feed, and fuels) requires reusing cultivation water to reduce operating costs. While some algae strains show growth inhibition in reused water, other strains appear un-affected. Reusing water to grow different strains (i.e., crop rotation) could potentially improve overall biomass production compared to water reuse with the same strain by reducing accumulation of strain-specific waste products, limiting carryover of population-specific pathogens such as viruses, and introducing bacteria that degrade residual organic matter. Here, batch culture experiments tested the effects of reused cultivation water from the self-inhibiting diatom Staurosira sp. C323 on the diatom Navicula sp. SFP and green alga Chlorella sp. D046, as well as their microbiomes. Navicula sp. and Chlorella sp. grew well in Staurosira sp. reused water, indicating that previously observed self-inhibition was likely specific to this Staurosira strain. While algal mi-crobiomes were not significantly different between controls and reused water treatments, specific bacteria taxa were differentially abundant in reused treatments, suggesting that some taxa responded to compounds re-maining in the reused water. Finally, results suggest that algae growth responses in reused water may depend largely on strain-specific factors, and that crop rotation can support biomass production.

View Accepted Manuscript (DOE)

Research Organization:: Duke Univ., Durham, NC (United States); Duke University, Durham, NC (United States)

Sponsoring Organization:: Gordon and Betty Moore Foundation; National Science Foundation (NSF); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: EE0007091; EE0008518

OSTI ID:: 1673383

Alternate ID(s):: OSTI ID: 2439749
OSTI ID: 1664465

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 51; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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