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Title: Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae)

Journal Article · · Applications in Plant Science
DOI:https://doi.org/10.1002/aps3.11273· OSTI ID:1670228
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [1]
  1. Key Laboratory of Resource Biology and Biotechnology in Western China Department of Life Science Northwest University Xi'an 710069 People's Republic of China
  2. Hainan Branch Institute of Medicinal Plant Development Peking Union Medical College and Chinese Academy of Medical Sciences Haikou Hainan 570311 People's Republic of China
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Premise The natural population size of Huperzia serrata (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat ( SSR ) markers for H. serrata to survey both its genetic diversity and population structure. Methods and Results Based on 177 individuals, 120 SSR primer pairs were developed and optimized from five regions of the H. serrata transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of H. serrata . Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in H. crispata , H. sutchueniana , and H. selago . Conclusions The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered H. serrata and other related species.

Sponsoring Organization:
USDOE
OSTI ID:
1670228
Alternate ID(s):
OSTI ID: 1670229
Journal Information:
Applications in Plant Science, Journal Name: Applications in Plant Science Vol. 7 Journal Issue: 7; ISSN 2168-0450
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
United States
Language:
English

References (8)

A community-derived classification for extant lycophytes and ferns: PPG I journal November 2016
Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) journal February 2003
Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis L.) journal January 2009
Identification of EST–SSRs and molecular diversity analysis in Mentha piperita journal August 2015
Comparison of 454-ESTs from Huperzia serrata and Phlegmariurus carinatus reveals putative genes involved in lycopodium alkaloid biosynthesis and developmental regulation journal January 2010
Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers journal April 2011
Involvement of Intracellular and Mitochondrial Aβ in the Ameliorative Effects of Huperzine A against Oligomeric Aβ42-Induced Injury in Primary Rat Neurons journal May 2015
Full-length transcriptome assembly from RNA-Seq data without a reference genome journal May 2011

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