BIN1 protein isoforms are differentially expressed in astrocytes, neurons, and microglia: neuronal and astrocyte BIN1 are implicated in tau pathology

Journal Article · Wed Jul 29 00:00:00 EDT 2020 · Molecular Neurodegeneration

DOI:https://doi.org/10.1186/s13024-020-00387-3· OSTI ID:1668667

Taga, Mariko ^[1]; Petyuk, Vladislav A.  ^[2]; White, Charles ^[1]; Marsh, Galina ^[3]; Ma, Yiyi ^[1]; Klein, Hans-Ulrich ^[1]; Connor, Sarah ^[1]; Kroshilina, Alexandra ^[1]; Yung, Christina ^[1]; Khairallah, Anthony ^[1]; Olah, Marta ^[1]; Schneider, Julie A. ^[4]; Karhohs, Kyle ^[5]; Carpenter, Anne ^[6]; Ransohoff, Richard ^[7]; Bennett, David A. ^[4]; Crotti, Andrea ^[8]; Bradshaw, Elizabeth ^[1]; De Jager, Philip L. ^[9]

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1. Columbia University
2. BATTELLE (PACIFIC NW LAB)
3. Biogen
4. Rush University
5. Harvard Medical School
6. Harvard University
7. Cleveland Clinic
8. Cambridge University
9. Broad Institute

Background: Identified as an Alzheimer’s disease (AD) susceptibility gene by genome wide-association studies, BIN1 has 10 isoforms that are expressed in the Central Nervous System (CNS). The distribution of these isoforms in different cell types, as well as their role in AD pathology still remains unclear. Methods: Utilizing antibodies targeting specific BIN1 epitopes in human post-mortem tissue and analyzing mRNA expression data from purified microglia, we identified three isoforms expressed in neurons and astrocytes (isoforms 1, 2 and 3) and four isoforms expressed in microglia (isoforms 6, 9, 10 and 12). The abundance of selected peptides, which correspond to groups of BIN1 protein isoforms, was measured in dorsolateral prefrontal cortex, and their relation to neuropathological features of AD was assessed. Results: Peptides contained in exon 7 of BIN1’s N-BAR domain were found to be significantly associated with ADrelated traits and, particularly, tau tangles. Decreased expression of BIN1 isoforms containing exon 7 is associated with greater accumulation of tangles and subsequent cognitive decline, with astrocytic rather than neuronal BIN1 being the more likely culprit. These effects are independent of the BIN1 AD risk variant. Conclusions: Exploring the molecular mechanisms of specific BIN1 isoforms expressed by astrocytes may open new avenues for modulating the accumulation of Tau pathology in AD. Keywords: BIN1 isoforms, Alzheimer’s disease, Amyloid, Tau, Microglia, Astrocytes, Neurons

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1668667
• Report Number(s): PNNL-SA-156642
• Journal Information: Molecular Neurodegeneration, Vol. 15, Issue 1; ISSN 1750--1326
• Country of Publication: United States
• Language: English