Chapter 16: Connecting Microbial Genotype with Phenotype in the Omics Era

Book · Tue Jul 28 04:00:00 EDT 2020

DOI:https://doi.org/10.1007/978-1-0716-0195-2_16· OSTI ID:1660186

Yang, Yongfu; Qiu, Mengyu; Yang, Qing; Wang, Yu; Wei, Hui; Yang, Shihui

Although rational design-based metabolic engineering has been applied widely to obtain promising microbial biocatalysts, conventional strategies such as adaptive laboratory evolution (ALE) and mutagenesis are still efficient approaches to improve microorganisms for exceptional features such as a broad spectrum of substrate utilization, robustness of cell growth, as well as high titer, yield, and productivity of the target products. In this chapter, we describe the procedure to generate mutant strains with desired phenotypes using ALE and a new mutagenesis approach of Atmosphere and Room Temperature Plasma (ARTP). In addition, we discuss the methodology to combine next-generation sequencing (NGS)-based genome-resequencing and RNA-Seq transcriptomics approaches to characterize the mutant strains and connect the phenotypes with their corresponding genotypic changes.

• Research Organization: National Renewable Energy Laboratory (NREL), Golden, CO (United States)
• Sponsoring Organization: USDOE Office of Energy Efficiency and Renewable Energy (EERE)
• DOE Contract Number: AC36-08GO28308
• OSTI ID: 1660186
• Report Number(s): NREL/CH-2700-77591; MainId:27527; UUID:95d33abd-6ea5-4d36-8239-36ab44dd1d47; MainAdminID:15257
• Country of Publication: United States
• Language: English

Related Subjects

BASIC BIOLOGICAL SCIENCES
BIOMASS FUELS
RNA-Seq
adaptive laboratory evolution (ALE)
atmosphere and room temperature plasma (ARTP) mutagenesis
next-generation sequencing (NGS)