Insights of Molecular Mechanism of Xylem Development in Five Black Poplar Cultivars

Zhang, Lei; Liu, Bobin; Zhang, Jin; Hu, Jianjun

doi:10.3389/fpls.2020.00620

Title: Insights of Molecular Mechanism of Xylem Development in Five Black Poplar Cultivars

Journal Article · Thu May 28 00:00:00 EDT 2020 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2020.00620· OSTI ID:1657938

Zhang, Lei ^[1]; Liu, Bobin ^[2];

^[3]; Hu, Jianjun ^[1]

Chinese Academy of Forestry, Beijing (China)
Fujian Agriculture and Forestry University, Fuzhou (China)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Black poplar (Populus deltoides, P. nigra, and their hybrids) is the main poplar cultivars in China. It offers interesting options of large-scale biomass production for bioenergy due to its rapid growth and high yield. Poplar wood properties were associated with chemical components and physical structures during wood formation. In this study, five poplar cultivars, P. euramericana ‘Zhonglin46’ (Pe1), P. euramericana ‘Guariento’ (Pe2), P. nigra ‘N179’ (Pn1), P. deltoides ‘Danhong’ (Pd1), and P. deltoides ‘Nanyang’ (Pd2), were used to explore the molecular mechanism of xylem development. We analyzed the structural differences of developing xylem in the five cultivars and profiled the transcriptome-wide gene expression patterns through RNA sequencing. The cross sections of the developing xylem showed that the cell wall thickness of developed fiber in Pd1 was thickest and the number of xylem vessels of Pn1 was the least. A total of 10,331 differentially expressed genes were identified among 10 pairwise comparisons of the five cultivars, most of them were related to programmed cell death and secondary cell wall thickening. K-means cluster analysis and Gene Ontology enrichment analysis showed that the genes highly expressed in Pd1 were related to nucleotide decomposition, metabolic process, transferase, and microtubule cytoskeleton; whereas the genes highly expressed in Pn1 were involved in cell wall macromolecule decomposition and polysaccharide binding processes. Based on a weighted gene co-expression network analysis, a large number of candidate regulators for xylem development were identified. And their potential regulatory roles to cell wall biosynthesis genes were validated by a transient overexpression system. This study provides a set of promising candidate regulators for genetic engineering to improve feedstock and enhance biofuel conversion in the bioenergy crop Populus.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1657938

Journal Information:: Frontiers in Plant Science, Vol. 11, Issue 1; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Insights of Molecular Mechanism of Xylem Development in Five Black Poplar Cultivars

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