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U.S. Department of Energy
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Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits

Patent ·
OSTI ID:1650762

Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-00OR22725
Assignee:
Trustees of Dartmouth College (Hanover, NH)
Patent Number(s):
10,619,172
Application Number:
15/656,665
OSTI ID:
1650762
Country of Publication:
United States
Language:
English