Understanding the dynamics of circulating tumor cell (CTC) behavior within the vasculature has remained an elusive goal in cancer biology. To elucidate the contribution of hydrodynamics in determining sites of CTC vascular colonization, the physical forces affecting these cells must be evaluated in a highly controlled manner. To this end, we have bioprinted endothelialized vascular beds and perfused these constructs with metastatic mammary gland cells under physiological flow rates. By pairing these in vitro devices with an advanced computational flow model, we found that the bioprinted analog was readily capable of evaluating the accuracy and integrated complexity of a computational flow model, while also highlighting the discrete contribution of hydrodynamics in vascular colonization. This intersection of these two technologies, bioprinting and computational simulation, is a key demonstration in the establishment of an experimentation pipeline for the understanding of complex biophysical events.
Hynes, W. F., et al. "Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model." Science Advances, vol. 6, no. 35, Aug. 2020. https://doi.org/10.1126/sciadv.abb3308
Hynes, W. F., Pepona, M., Robertson, C., Alvarado, J., Dubbin, K., Triplett, M., Adorno, J. J., Randles, A., & Moya, M. L. (2020). Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model. Science Advances, 6(35). https://doi.org/10.1126/sciadv.abb3308
Hynes, W. F., Pepona, M., Robertson, C., et al., "Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model," Science Advances 6, no. 35 (2020), https://doi.org/10.1126/sciadv.abb3308
@article{osti_1650706,
author = {Hynes, W. F. and Pepona, M. and Robertson, C. and Alvarado, J. and Dubbin, K. and Triplett, M. and Adorno, J. J. and Randles, A. and Moya, M. L.},
title = {Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model},
annote = {Understanding the dynamics of circulating tumor cell (CTC) behavior within the vasculature has remained an elusive goal in cancer biology. To elucidate the contribution of hydrodynamics in determining sites of CTC vascular colonization, the physical forces affecting these cells must be evaluated in a highly controlled manner. To this end, we have bioprinted endothelialized vascular beds and perfused these constructs with metastatic mammary gland cells under physiological flow rates. By pairing these in vitro devices with an advanced computational flow model, we found that the bioprinted analog was readily capable of evaluating the accuracy and integrated complexity of a computational flow model, while also highlighting the discrete contribution of hydrodynamics in vascular colonization. This intersection of these two technologies, bioprinting and computational simulation, is a key demonstration in the establishment of an experimentation pipeline for the understanding of complex biophysical events.},
doi = {10.1126/sciadv.abb3308},
url = {https://www.osti.gov/biblio/1650706},
journal = {Science Advances},
issn = {ISSN 2375-2548},
number = {35},
volume = {6},
place = {United States},
publisher = {AAAS},
year = {2020},
month = {08}}
Randles, Amanda Peters; Kale, Vivek; Hammond, Jeff
2013 IEEE International Symposium on Parallel & Distributed Processing (IPDPS), 2013 IEEE 27th International Symposium on Parallel and Distributed Processinghttps://doi.org/10.1109/IPDPS.2013.109
2010 SC - International Conference for High Performance Computing, Networking, Storage and Analysis, 2010 ACM/IEEE International Conference for High Performance Computing, Networking, Storage and Analysishttps://doi.org/10.1109/SC.2010.33