Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate

Schambach, Jenna Y.; Finck, Anna M.; Kitin, Peter; Hunt, Christopher G.; Hanschen, Erik R.; Vogler, Brian; Starkenburg, Shawn R.; Barry, Amanda N.

doi:10.1016/j.algal.2020.102041

Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate

Journal Article · Thu Oct 01 00:00:00 EDT 2020 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2020.102041· OSTI ID:1650143

Schambach, Jenna Y. ^[1]; Finck, Anna M. ^[1]; Kitin, Peter ^[2]; Hunt, Christopher G. ^[2]; Hanschen, Erik R. ^[1]; Vogler, Brian ^[3]; Starkenburg, Shawn R. ^[1]; Barry, Amanda N. ^[4]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
U.S. Forest Service, Madison, WI (United States). Forest Products Lab.
Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Conagen, Bedford, MA (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Improving productivity and lipid concentration in microalgae is important for the economic success of both biofuel and microalgae coproducts production. Nannochloropsis spp. are marine microalgae currently being grown at large-scale for the production of biofuel and lipid coproducts. In this work, we demonstrate improvements of growth and omega-3 production in Nannochloropsis gaditana CCMP526 and Nannochloropsis oceanica CCAP849/10 with plant substrate addition, a potentially economical option for increasing microalgal productivity. Furthermore, we examine growth in the presence of corn stover, switchgrass, sugarcane bagasse, and yard waste. By examining the microbial ecology of N. gaditana cultures with and without plant substrate and with and without antibiotics, we discovered a potential bacterial interaction in these cultures, but its presence is not necessary for algal growth improvements in the presence of plant substrate. Analysis of plant substrate morphology by scanning electron microscopy (SEM) after cultivation in media with and without N. gaditana shows a degradation of specific plant structural features and a colonization of the plant phloem by N. gaditana. An examination of N. gaditana in a Congo red plate assay indicates potential cellulolytic activity, but a preliminary examination of the potential cellulase transcripts does not reveal differential expression of a candidate in the presence of plant. This evidence demonstrates potential raw plant utilization by a marine microalga for increased productivity and provides a potentially economical option for increasing coproduct concentration at industrial scale without genetic engineering.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC04-94AL85000; 89233218CNA000001

OSTI ID:: 1650143

Alternate ID(s):: OSTI ID: 1811141
OSTI ID: 1739994

Report Number(s):: SAND--2020-7729J; 687673

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 51; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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