The effect of lignin degradation products on the generation of pseudo-lignin during dilute acid pretreatment

He, Juan; Huang, Caoxing; Lai, Chenhuan; Huang, Chen; Li, Mi; Pu, Yunqiao Joseph; Ragauskas, Arthur J.; Yong, Qiang

doi:10.1016/j.indcrop.2020.112205

The effect of lignin degradation products on the generation of pseudo-lignin during dilute acid pretreatment

Journal Article · Wed Feb 12 00:00:00 EST 2020 · Industrial Crops and Products

DOI:https://doi.org/10.1016/j.indcrop.2020.112205· OSTI ID:1649308

He, Juan ^[1]; Huang, Caoxing ^[1]; Lai, Chenhuan ^[1]; Huang, Chen ^[1]; ^[2]; ^[3]; ^[2]; Yong, Qiang ^[1]

Nanjing Forestry Univ. (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Pseudo-lignin is an insoluble material that tends to deposit on acidic pretreated fiber surface and negatively impacts the biological conversion of biomass to valued added products. Employing a laboratory generated acidic hydrolysate solution from dilute acid pretreatment of bamboo residues, we sought to seek an improved understanding of pseudo-lignin formation from fragmented carbohydrates. In addition, we also performed experiments to investigate how lignin model compounds interact with carbohydrates en route to pseudo-lignin’s formation. The yields of pseudo-lignin generated from xylose (CXPL) were higher than those generated from glucose (CGPL). Gas chromatography-Mass spectrometry and Foloin-Ciocalteau analysis suggested that there were more aromatic compounds in hydrolyzate during CGPL’s formation. The presence of lignin phenolic model compounds had both a positive and negative effect upon quantitative yields of pseudo-lignin. Gel permeation chromatography and NMR analysis (³¹P and 2D-HSQC NMR) of the pseudo-lignin samples generated in the presence and absence of 4-hydroxybenzoic acid (4-HA), employed as a lignin model compound, revealed drastic differences in molecular weight, changes in hydroxyl group content, and hydrocarbon bonds, suggesting significant differences in the pseudo-lignin reaction pathways when certain lignin model compounds are present. Finally, our findings demonstrate the effects of both hemicellulose carbohydrate makeup and lignin model compound impact on the formation of pseudo-lignin and its chemical properties, suggesting a potential way to minimize pseudo-lignin formation.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1649308

Journal Information:: Industrial Crops and Products, Journal Name: Industrial Crops and Products Vol. 146; ISSN 0926-6690

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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phenolic compound
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The effect of lignin degradation products on the generation of pseudo-lignin during dilute acid pretreatment

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