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Geochemistry, RNA radiocarbon signature, and metatranscriptome data from Rifle, Colorado groundwater well

Dataset ·
DOI:https://doi.org/10.15485/1647359· OSTI ID:1647359
 [1];  [2];  [2];  [2];  [2];  [2];  [2];  [3];  [4];  [4];  [4];  [4]
  1. Barnard College; Lawrence Berkeley National Laboratory
  2. Lawrence Berkeley National Laboratory
  3. Lawrence Livermore National Laboratory
  4. Barnard College
+ Show Author Affiliations

Understanding the different sources of carbon subsurface microbes use is highly informative yet remains challenging. The goal of this work was to develop and test a cultivation-independent method that analyzes both the radiocarbon and ribosomal RNA (rRNA) signature of unamplified RNA from the same environmental sample, and that collectively characterizes community carbon utilization under in situ conditions. The method was applied to a filtered groundwater sample from a geochemically reduced zone of a shallow alluvial aquifer near Rife, Colorado (CO), where multiple potential carbon sources co-occur. The radiocarbon and RNA-Seq results, while addressing diverse sample properties, indicated a strikingly similar conclusion about the quantitative role of autotrophy in the subsurface microbial community.This data package provides (1) ribosomal RNA (rRNA) sequences for groundwater microbial populations, (2) the taxonomic identity and relative abundance of groundwater microbial populations based on these rRNA sequences, (3) radiocarbon signatures of the rRNA indicating the source of carbon used by the groundwater microbial populations (provided in both Word docx file and pdf file), (4) geochemical measurements for the monitoring well corresponding to these measurements.

Research Organization:
Environmental System Science Data Infrastructure for a Virtual Ecosystem; Watershed Function SFA
Sponsoring Organization:
U.S. DOE > Office of Science > Biological and Environmental Research (BER)
OSTI ID:
1647359
Report Number(s):
paf_454
Country of Publication:
United States
Language:
English

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Related Subjects

54 ENVIRONMENTAL SCIENCES
EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > GROUND WATER > GROUNDWATER CHEMISTRY
Isotopes
arsenic concentration
chloride concentration
dissolved inorganic carbon
dissolved organic carbon
dissolved oxygen
iron concentration
magnesium concentration
metatranscriptomics
microbiome
pH
radiocarbon analysis
sulfate concentration