Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds

Saint-Vincent, Patricia MB; Ridout, Mary; Engle, Nancy L.; Lawrence, Travis J.; Yeary, Meredith L.; Tschaplinski, Timothy J.; Newcombe, George; Pelletier, Dale A.

doi:10.3390/microorganisms8081137

Title: Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds

Journal Article · Tue Jul 28 00:00:00 EDT 2020 · Microorganisms

DOI:https://doi.org/10.3390/microorganisms8081137· OSTI ID:1643739

Saint-Vincent, Patricia MB; Ridout, Mary; Engle, Nancy L.;

; Yeary, Meredith L.;

;

; Pelletier, Dale A.

Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populustrichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Nuclear Energy (NE)

Grant/Contract Number:: SC0014547; AC05-00OR22725

OSTI ID:: 1643739

Alternate ID(s):: OSTI ID: 1649637

Journal Information:: Microorganisms, Journal Name: Microorganisms Vol. 8 Journal Issue: 8; ISSN 2076-2607

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

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