Coping with Starvation: Contrasting Lipidomic Dynamics in the Cells of Two Sacoglossan Sea Slugs Incorporating Stolen Plastids from the Same Macroalga

Rey, Felisa; Melo, Tânia; Cartaxana, Paulo; Calado, Ricardo; Domingues, Pedro; Cruz, Sónia; Domingues, M. Rosário M.

doi:10.1093/icb/icaa019

Title: Coping with Starvation: Contrasting Lipidomic Dynamics in the Cells of Two Sacoglossan Sea Slugs Incorporating Stolen Plastids from the Same Macroalga

Journal Article · Wed Apr 15 00:00:00 EDT 2020 · Integrative and Comparative Biology

DOI:https://doi.org/10.1093/icb/icaa019· OSTI ID:1642251

Rey, Felisa ^[1]; Melo, Tânia ^[1]; Cartaxana, Paulo ^[2]; Calado, Ricardo ^[2]; Domingues, Pedro ^[3]; Cruz, Sónia ^[2]; Domingues, M. Rosário M. ^[1]

CESAM, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal, Mass Spectrometry Centre & QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal
ECOMARE, CESAM, Department of Biology, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal
Mass Spectrometry Centre & QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal

Abstract Several species of sacoglossan sea slugs are able to sequester chloroplasts from algae and incorporate them into their cells. However, the ability to maintain functional “stolen” plastids (kleptoplasts) can vary significantly within the Sacoglossa, giving species different capacities to withstand periods of food shortage. The present study provides an insight on the comparative shifts experienced by the lipidome of two sacoglossan sea slug species, Elysia viridis (long-term retention of functional chloroplasts) and Placida dendritica (retention of non-functional chloroplasts). A hydrophilic interaction liquid chromatography–mass spectrometry approach was employed to screen the lipidome of specimens from both species feeding on the macroalga Codium tomentosum and after 1-week of starvation. The lipidome of E. viridis was generally unaffected by the absence of food, while that of P. dendritica varied significantly. The retention of functional chloroplasts by E. viridis cells allows this species to endure periods of food shortage, while in P. dendritica a significant reduction in the amount of main lipids was the consequence of the consumption of its own mass to endure starvation. The large proportion of ether phospholipids (plasmalogens) in both sea slug species suggests that these compounds may play a key role in chloroplast incorporation in sea slug cells and/or be involved in the reduction of the oxidative stress resulting from the presence of kleptoplasts.

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Sponsoring Organization:: USDOE Office of Nuclear Energy (NE), Nuclear Fuel Cycle and Supply Chain

Grant/Contract Number:: CEECIND/00580/2017; IF/00899/2014; PTDC/BIA-ANM/4622/2014; POCI-01-0145-FEDER-016754; PTDC/BIA-FBT/30979/2017

OSTI ID:: 1642251

Journal Information:: Integrative and Comparative Biology, Journal Name: Integrative and Comparative Biology Vol. 60 Journal Issue: 1; ISSN 1540-7063

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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