Assessment of the Utility of the Oral Fluid and Plasma Proteome for Hydrocodone Usage

Kaiser, Brooke LD; Jacobs, Jon M.; Schepmoes, Athena A.; Brewer, Heather M.; Webb-Robertson, Bobbie-Jo M.; Valtier, Sandra; Bebarta, Vikhyat S.; Adkins, Joshua N.

doi:10.1007/s13181-019-00731-0

Assessment of the Utility of the Oral Fluid and Plasma Proteome for Hydrocodone Usage

Journal Article · Thu Jan 02 04:00:00 EST 2020 · Journal of Medical Toxicology

DOI:https://doi.org/10.1007/s13181-019-00731-0· OSTI ID:1640990

Kaiser, Brooke LD ^[1]; Jacobs, Jon M. ^[1]; Schepmoes, Athena A. ^[1]; ^[1]; Webb-Robertson, Bobbie-Jo M. ^[1]; Valtier, Sandra ^[2]; Bebarta, Vikhyat S. ^[3]; ^[1]

BATTELLE (PACIFIC NW LAB)
Wilford Hall Medical Center
University of Colorado at Denver

Non-medical use and abuse of prescription opioids is a growing problem in both the civilian and military communities, with minimal technologies for detecting hydrocodone use. This study explored the proteomic changes that occur in the oral fluid and blood plasma following controlled hydrocodone administration in twenty subjects. The global proteomic profile was determined for samples taken at four time points per subject of pre-exposure and 4, 6, or 168 hours post-exposure. The oral fluid samples analyzed herein provided greater differentiation between “baseline” and “response” time points than was observed with blood plasma, at least partially due to significant person-to-person variability in the plasma proteome. A total of 399 proteins were identified from oral fluid samples, and the abundance of 102 of those proteins was determined to be significantly different upon metabolism of hydrocodone (4 and 6 hour time points) as compared to baseline levels in the oral fluid (pre-dose and 168 hour). This is the first, to our knowledge, assessment of the oral fluid and blood plasma proteome following hydrocodone administration, which demonstrates the potential of oral fluid as a non-invasive sample that may reveal features of hydrocodone or other opioid use and/or misuse.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1640990

Report Number(s):: PNNL-SA-121218

Journal Information:: Journal of Medical Toxicology, Journal Name: Journal of Medical Toxicology Journal Issue: 1 Vol. 16

Country of Publication:: United States

Language:: English

References (27)

Characterization of Strategies for Obtaining Confident Identifications in Bottom-Up Proteomics Measurements Using Hybrid FTMS Instruments Tolmachev, Aleksey V.; Monroe, Matthew E.; Purvine, Samuel O. Analytical Chemistry, Vol. 80, Issue 22 https://doi.org/10.1021/ac801376g	journal	November 2008
Characterization of salivary proteins of schizophrenic and bipolar disorder patients by top-down proteomics Iavarone, Federica; Melis, Marianna; Platania, Giovanna Journal of Proteomics, Vol. 103 https://doi.org/10.1016/j.jprot.2014.03.020	journal	May 2014
Quantitative method for analysis of hydrocodone, hydromorphone and norhydrocodone in human plasma by liquid chromatography–tandem mass spectrometry Valtier, Sandra; Mueck, Robert L.; Bebarta, Vikhyat S. Journal of Chromatography B, Vol. 925 https://doi.org/10.1016/j.jchromb.2013.02.030	journal	April 2013
Human salivary proteome — a resource of potential biomarkers for oral cancer Sivadasan, Priya; Gupta, Manoj Kumar; Sathe, Gajanan J. Journal of Proteomics, Vol. 127 https://doi.org/10.1016/j.jprot.2015.05.039	journal	September 2015
Presentation of Prescription and Nonprescription Opioid Overdoses to US Emergency Departments Yokell, Michael A.; Delgado, M. Kit; Zaller, Nickolas D. JAMA Internal Medicine, Vol. 174, Issue 12 https://doi.org/10.1001/jamainternmed.2014.5413	journal	December 2014
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801 https://doi.org/10.1021/ac061133r	journal	November 2006
The New Substance Abuse and Mental Health Services Administration Oral Fluid Cutoffs for Cocaine and Heroin-Related Analytes Applied to an Addiction Medicine Setting: Important, Unanticipated Findings with LC-MS/MS Flood, James G.; Khaliq, Tahira; Bishop, Kenneth A. Clinical Chemistry, Vol. 62, Issue 5 https://doi.org/10.1373/clinchem.2015.251066	journal	May 2016
Developments in FTICR-MS and Its Potential for Body Fluid Signatures Nicolardi, Simone; Bogdanov, Bogdan; Deelder, André International Journal of Molecular Sciences, Vol. 16, Issue 11 https://doi.org/10.3390/ijms161126012	journal	November 2015
Emergency medicine providers' opioid prescribing practices stratified by gender, age, and years in practice Varney, Shawn M. World Journal of Emergency Medicine, Vol. 7, Issue 2 https://doi.org/10.5847/wjem.j.1920-8642.2016.02.004	journal	January 2016
CDC Guideline for Prescribing Opioids for Chronic Pain—United States, 2016 Dowell, Deborah; Haegerich, Tamara M.; Chou, Roger JAMA, Vol. 315, Issue 15 https://doi.org/10.1001/jama.2016.1464	journal	April 2016
Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids Wu, Chaochao; Duan, Jicheng; Liu, Tao Journal of Chromatography B, Vol. 1021 https://doi.org/10.1016/j.jchromb.2016.01.015	journal	May 2016
The human salivary proteome: a critical overview of the results obtained by different proteomic platforms Castagnola, Massimo; Cabras, Tiziana; Iavarone, Federica Expert Review of Proteomics, Vol. 9, Issue 1 https://doi.org/10.1586/epr.11.77	journal	February 2012
Utilizing Human Blood Plasma for Proteomic Biomarker Discovery ^† Jacobs, Jon M.; Adkins, Joshua N.; Qian, Wei-Jun Journal of Proteome Research, Vol. 4, Issue 4 https://doi.org/10.1021/pr0500657	journal	August 2005
VIPER: an advanced software package to support high-throughput LC-MS peptide identification Monroe, Matthew E.; Tolić, Nikola; Jaitly, Navdeep Bioinformatics, Vol. 23, Issue 15 https://doi.org/10.1093/bioinformatics/btm281	journal	June 2007
A Statistical Method for Assessing Peptide Identification Confidence in Accurate Mass and Time Tag Proteomics Stanley, Jeffrey R.; Adkins, Joshua N.; Slysz, Gordon W. Analytical Chemistry, Vol. 83, Issue 16 https://doi.org/10.1021/ac2009806	journal	August 2011
DAnTE: a statistical tool for quantitative analysis of -omics data Polpitiya, A. D.; Qian, W. -J.; Jaitly, N. Bioinformatics, Vol. 24, Issue 13 https://doi.org/10.1093/bioinformatics/btn217	journal	May 2008
Proteomic Analysis of Saliva in HIV-Positive Heroin Addicts Reveals Proteins Correlated with Cognition Dominy, Stephen S.; Brown, Joseph N.; Ryder, Mark I. PLoS ONE, Vol. 9, Issue 4 https://doi.org/10.1371/journal.pone.0089366	journal	April 2014
White House expands opioid addiction response Traynor, Kate American Journal of Health-System Pharmacy, Vol. 73, Issue 1 https://doi.org/10.2146/news160001	journal	January 2016
High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis Yang, Feng; Shen, Yufeng; Camp, David G. Expert Review of Proteomics, Vol. 9, Issue 2 https://doi.org/10.1586/epr.12.15	journal	April 2012
Impact of an Opioid Prescribing Guideline in the Acute Care Setting del Portal, Daniel A.; Healy, Megan E.; Satz, Wayne A. The Journal of Emergency Medicine, Vol. 50, Issue 1 https://doi.org/10.1016/j.jemermed.2015.06.014	journal	January 2016
DeconMSn: a software tool for accurate parent ion monoisotopic mass determination for tandem mass spectra Mayampurath, A. M.; Jaitly, N.; Purvine, S. O. Bioinformatics, Vol. 24, Issue 7 https://doi.org/10.1093/bioinformatics/btn063	journal	February 2008
Ultra-deep and quantitative saliva proteome reveals dynamics of the oral microbiome Grassl, Niklas; Kulak, Nils Alexander; Pichler, Garwin Genome Medicine, Vol. 8, Issue 1 https://doi.org/10.1186/s13073-016-0293-0	journal	April 2016
Revisiting biomarker discovery by plasma proteomics Geyer, Philipp E.; Holdt, Lesca M.; Teupser, Daniel Molecular Systems Biology, Vol. 13, Issue 9 https://doi.org/10.15252/msb.20156297	journal	September 2017
Self-made frits for nanoscale columns in proteomics Maiolica, Alessio; Borsotti, Dario; Rappsilber, Juri PROTEOMICS, Vol. 5, Issue 15 https://doi.org/10.1002/pmic.200402010	journal	October 2005
Saliva: a Dynamic Proteome Helmerhorst, E. J.; Oppenheim, F. G. Journal of Dental Research, Vol. 86, Issue 8 https://doi.org/10.1177/154405910708600802	journal	August 2007
Fast and Accurate Database Searches with MS-GF+Percolator Granholm, Viktor; Kim, Sangtae; Navarro, José C. F. Journal of Proteome Research, Vol. 13, Issue 2 https://doi.org/10.1021/pr400937n	journal	December 2013
Applying a Targeted Label-Free Approach Using LC−MS AMT Tags to Evaluate Changes in Protein Phosphorylation Following Phosphatase Inhibition Yang, Feng; Jaitly, Navdeep; Jayachandran, Hemalatha Journal of Proteome Research, Vol. 6, Issue 11 https://doi.org/10.1021/pr070068e	journal	November 2007

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Assessment of the Utility of the Oral Fluid and Plasma Proteome for Hydrocodone Usage

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