The CCR4‐NOT complex component NOT1 regulates RNA‐directed DNA methylation and transcriptional silencing by facilitating Pol IV‐dependent siRNA production

Zhou, Hao‐Ran; Lin, Rong‐Nan; Huang, Huan‐Wei; Li, Lin; Cai, Tao; Zhu, Jian‐Kang; Chen, She; He, Xin‐Jian

doi:10.1111/tpj.14818

Title: The CCR4‐NOT complex component NOT1 regulates RNA‐directed DNA methylation and transcriptional silencing by facilitating Pol IV‐dependent siRNA production

Journal Article · Thu Jun 25 00:00:00 EDT 2020 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.14818· OSTI ID:1635067

Zhou, Hao‐Ran ^[1]; Lin, Rong‐Nan ^[1]; Huang, Huan‐Wei ^[1]; Li, Lin ^[1]; Cai, Tao ^[1]; Zhu, Jian‐Kang ^[2]; Chen, She ^[1];

^[3]

National Institute of Biological Sciences Beijing 102206 China
Shanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China
National Institute of Biological Sciences Beijing 102206 China, Tsinghua Institute of Multidisciplinary Biomedical Research Tsinghua University Beijing China

Summary Small interfering RNAs (siRNAs) are responsible for establishing and maintaining DNA methylation through the RNA‐directed DNA methylation (RdDM) pathway in plants. Although siRNA biogenesis is well known, it is relatively unclear about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana , we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. We demonstrated that NOT1 is required for Pol IV‐dependent siRNA accumulation and DNA methylation at a subset of RdDM target genomic loci. Furthermore, we revealed that NOT1 is a constituent of a multi‐subunit CCR4‐NOT deadenylase complex by immunoprecipitation combined with mass spectrometry and demonstrated that the CCR4‐NOT components can function as a whole to mediate chromatin silencing. Therefore, our work establishes that the CCR4‐NOT complex regulates the biogenesis of Pol IV‐dependent siRNAs, and hence facilitates DNA methylation and transcriptional silencing in Arabidopsis.

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Sponsoring Organization:: USDOE

OSTI ID:: 1635067

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Vol. 103 Journal Issue: 4; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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References (75)

Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Zhou, Ming; Palanca, Ana Marie S.; Law, Julie A. Nature Genetics, Vol. 50, Issue 6 https://doi.org/10.1038/s41588-018-0115-y	journal	May 2018
The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs Dou, K.; Huang, C. -F.; Ma, Z. -Y. Nucleic Acids Research, Vol. 41, Issue 18 https://doi.org/10.1093/nar/gkt639	journal	July 2013
An atypical component of RNA-directed DNA methylation machinery has both DNA methylation-dependent and -independent roles in locus-specific transcriptional gene silencing Liu, Jun; Bai, Ge; Zhang, Cuijun Cell Research, Vol. 21, Issue 12 https://doi.org/10.1038/cr.2011.173	journal	November 2011
Complexity of the Alternative Splicing Landscape in Plants Reddy, Anireddy S. N.; Marquez, Yamile; Kalyna, Maria The Plant Cell, Vol. 25, Issue 10 https://doi.org/10.1105/tpc.113.117523	journal	October 2013
Identification of Arabidopsis CCR4-NOT Complexes with Pumilio RNA-Binding Proteins, APUM5 and APUM2 Arae, Toshihiro; Morita, Kotone; Imahori, Riko Plant and Cell Physiology, Vol. 60, Issue 9 https://doi.org/10.1093/pcp/pcz089	journal	May 2019
Mechanisms of deadenylation-dependent decay: Mechanisms of deadenylation-dependent decay Chen, Chyi-Ying A.; Shyu, Ann-Bin Wiley Interdisciplinary Reviews: RNA, Vol. 2, Issue 2 https://doi.org/10.1002/wrna.40	journal	September 2010
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Langmead, Ben; Trapnell, Cole; Pop, Mihai Genome Biology, Vol. 10, Issue 3 https://doi.org/10.1186/gb-2009-10-3-r25	journal	January 2009
SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis Han, Yong-Feng; Dou, Kun; Ma, Ze-Yang Cell Research, Vol. 24, Issue 12 https://doi.org/10.1038/cr.2014.156	journal	November 2014
The Ccr4–Not complex Collart, Martine A.; Panasenko, Olesya O. Gene, Vol. 492, Issue 1 https://doi.org/10.1016/j.gene.2011.09.033	journal	January 2012
The Not3/5 subunit of the Ccr4-Not complex: A central regulator of gene expression that integrates signals between the cytoplasm and the nucleus in eukaryotic cells Collart, Martine A.; Panasenko, Olesya O.; Nikolaev, Sergey I. Cellular Signalling, Vol. 25, Issue 4 https://doi.org/10.1016/j.cellsig.2012.12.018	journal	April 2013
Establishing epigenetic variation during genome reprogramming Borges, Filipe; Martienssen, Robert A. RNA Biology, Vol. 10, Issue 4 https://doi.org/10.4161/rna.24085	journal	April 2013
RNAi triggered by specialized machinery silences developmental genes and retrotransposons Yamanaka, Soichiro; Mehta, Sameet; Reyes-Turcu, Francisca E. Nature, Vol. 493, Issue 7433 https://doi.org/10.1038/nature11716	journal	November 2012
Multifunctional roles of the mammalian CCR4â€“NOT complex in physiological phenomena Shirai, Yo-Taro; Suzuki, Toru; Morita, Masahiro Frontiers in Genetics, Vol. 5 https://doi.org/10.3389/fgene.2014.00286	journal	August 2014
Comprehensive Analysis of Silencing Mutants Reveals Complex Regulation of the Arabidopsis Methylome Stroud, Hume; Greenberg, Maxim V. C.; Feng, Suhua Cell, Vol. 152, Issue 1-2 https://doi.org/10.1016/j.cell.2012.10.054	journal	January 2013
An Effector of RNA-Directed DNA Methylation in Arabidopsis Is an ARGONAUTE 4- and RNA-Binding Protein He, Xin-Jian; Hsu, Yi-Feng; Zhu, Shihua Cell, Vol. 137, Issue 3 https://doi.org/10.1016/j.cell.2009.04.028	journal	May 2009
The splicing machinery promotes RNA-directed DNA methylation and transcriptional silencing in Arabidopsis Zhang, Cui-Jun; Zhou, Jin-Xing; Liu, Jun The EMBO Journal, Vol. 32, Issue 8 https://doi.org/10.1038/emboj.2013.49	journal	March 2013
The SUMO E3 Ligase-Like Proteins PIAL1 and PIAL2 Interact with MOM1 and Form a Novel Complex Required for Transcriptional Silencing Han, Yong-Feng; Zhao, Qiu-Yuan; Dang, Liang-Liang The Plant Cell, Vol. 28, Issue 5 https://doi.org/10.1105/tpc.15.00997	journal	April 2016
Dynamics and function of DNA methylation in plants Zhang, Huiming; Lang, Zhaobo; Zhu, Jian-Kang Nature Reviews Molecular Cell Biology, Vol. 19, Issue 8 https://doi.org/10.1038/s41580-018-0016-z	journal	May 2018
Folate Polyglutamylation Is Involved in Chromatin Silencing by Maintaining Global DNA Methylation and Histone H3K9 Dimethylation in Arabidopsis Zhou, H. -R.; Zhang, F. -F.; Ma, Z. -Y. The Plant Cell, Vol. 25, Issue 7 https://doi.org/10.1105/tpc.113.114678	journal	July 2013
The Arabidopsis Chromatin-Modifying Nuclear siRNA Pathway Involves a Nucleolar RNA Processing Center Pontes, Olga; Li, Carey Fei; Nunes, Pedro Costa Cell, Vol. 126, Issue 1 https://doi.org/10.1016/j.cell.2006.05.031	journal	July 2006
RNA-directed DNA methylation: an epigenetic pathway of increasing complexity Matzke, Marjori A.; Mosher, Rebecca A. Nature Reviews Genetics, Vol. 15, Issue 6 https://doi.org/10.1038/nrg3683	journal	May 2014
Arabidopsis Deadenylases AtCAF1a and AtCAF1b Play Overlapping and Distinct Roles in Mediating Environmental Stress Responses Walley, Justin W.; Kelley, Dior R.; Nestorova, Gergana Plant Physiology, Vol. 152, Issue 2 https://doi.org/10.1104/pp.109.149005	journal	December 2009
On the importance of being co-transcriptional Neugebauer, K. M. Journal of Cell Science, Vol. 115, Issue 20 https://doi.org/10.1242/jcs.00073	journal	October 2002
Structural and Biochemical Insights to the Role of the CCR4-NOT Complex and DDX6 ATPase in MicroRNA Repression Mathys, Hansruedi; Basquin, Jérôme; Ozgur, Sevim Molecular Cell, Vol. 54, Issue 5 https://doi.org/10.1016/j.molcel.2014.03.036	journal	June 2014
DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV Zhang, H.; Ma, Z. -Y.; Zeng, L. Proceedings of the National Academy of Sciences, Vol. 110, Issue 20 https://doi.org/10.1073/pnas.1300585110	journal	May 2013
The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis Ausin, Israel; Greenberg, Maxim V. C.; Li, Carey Fei Epigenetics, Vol. 7, Issue 1 https://doi.org/10.4161/epi.7.1.18782	journal	January 2012
AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development Motomura, Kazuki; Arae, Toshihiro; Araki-Uramoto, Haruka Plant and Cell Physiology, Vol. 61, Issue 4 https://doi.org/10.1093/pcp/pcz235	journal	October 2019
Novel roles of the multi-functional CCR4-NOT complex in post-transcriptional regulation Inada, Toshifumi; Makino, Shiho Frontiers in Genetics, Vol. 5 https://doi.org/10.3389/fgene.2014.00135	journal	May 2014
Splicing Factors Facilitate RNAi-Directed Silencing in Fission Yeast Bayne, E. H.; Portoso, M.; Kagansky, A. Science, Vol. 322, Issue 5901 https://doi.org/10.1126/science.1164029	journal	October 2008
DNA methylation pathways and their crosstalk with histone methylation Du, Jiamu; Johnson, Lianna M.; Jacobsen, Steven E. Nature Reviews Molecular Cell Biology, Vol. 16, Issue 9 https://doi.org/10.1038/nrm4043	journal	August 2015
Multisubunit RNA polymerases IV and V: purveyors of non-coding RNA for plant gene silencing Haag, Jeremy R.; Pikaard, Craig S. Nature Reviews Molecular Cell Biology, Vol. 12, Issue 8 https://doi.org/10.1038/nrm3152	journal	July 2011
Non-Coding RNA Transcription and RNA-Directed DNA Methylation in Arabidopsis He, Xin-Jian; Ma, Ze-Yang; Liu, Zhang-Wei Molecular Plant, Vol. 7, Issue 9 https://doi.org/10.1093/mp/ssu075	journal	September 2014
SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation Johnson, Lianna M.; Du, Jiamu; Hale, Christopher J. Nature, Vol. 507, Issue 7490 https://doi.org/10.1038/nature12931	journal	January 2014
A Two-Step Process for Epigenetic Inheritance in Arabidopsis Blevins, Todd; Pontvianne, Frédéric; Cocklin, Ross Molecular Cell, Vol. 54, Issue 1 https://doi.org/10.1016/j.molcel.2014.02.019	journal	April 2014
Establishing, maintaining and modifying DNA methylation patterns in plants and animals Law, Julie A.; Jacobsen, Steven E. Nature Reviews Genetics, Vol. 11, Issue 3 https://doi.org/10.1038/nrg2719	journal	February 2010
ROS1, a Repressor of Transcriptional Gene Silencing in Arabidopsis, Encodes a DNA Glycosylase/Lyase Gong, Zhizhong; Morales-Ruiz, Teresa; Ariza, Rafael R. Cell, Vol. 111, Issue 6 https://doi.org/10.1016/S0092-8674(02)01133-9	journal	December 2002
A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis Ye, Ruiqiang; Chen, Zulong; Lian, Bi Molecular Cell, Vol. 61, Issue 2 https://doi.org/10.1016/j.molcel.2015.11.015	journal	January 2016
Identification of Pol IV and RDR2-dependent precursors of 24 nt siRNAs guiding de novo DNA methylation in Arabidopsis Blevins, Todd; Podicheti, Ram; Mishra, Vibhor eLife, Vol. 4 https://doi.org/10.7554/eLife.09591	journal	October 2015
Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1 Law, Julie A.; Du, Jiamu; Hale, Christopher J. Nature, Vol. 498, Issue 7454 https://doi.org/10.1038/nature12178	journal	May 2013
Insights into the structure of the CCR4-NOT complex by electron microscopy Nasertorabi, Fariborz; Batisse, Claire; Diepholz, Meikel FEBS Letters, Vol. 585, Issue 14 https://doi.org/10.1016/j.febslet.2011.05.071	journal	June 2011
Role of RNA polymerase IV in plant small RNA metabolism Zhang, X.; Henderson, I. R.; Lu, C. Proceedings of the National Academy of Sciences, Vol. 104, Issue 11 https://doi.org/10.1073/pnas.0611456104	journal	March 2007
Ccr4-Not complex: the control freak of eukaryotic cells Miller, Jason E.; Reese, Joseph C. Critical Reviews in Biochemistry and Molecular Biology, Vol. 47, Issue 4 https://doi.org/10.3109/10409238.2012.667214	journal	March 2012
Tandem repeats upstream of the Arabidopsis endogene SDC recruit non-CG DNA methylation and initiate siRNA spreading Henderson, I. R.; Jacobsen, S. E. Genes & Development, Vol. 22, Issue 12 https://doi.org/10.1101/gad.1667808	journal	June 2008
RNA decay machines: Deadenylation by the Ccr4–Not and Pan2–Pan3 complexes Wahle, Elmar; Winkler, G. Sebastiaan Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Vol. 1829, Issue 6-7 https://doi.org/10.1016/j.bbagrm.2013.01.003	journal	June 2013
Molecular Mechanism of Action of Plant DRM De Novo DNA Methyltransferases Zhong, Xuehua; Du, Jiamu; Hale, Christopher J. Cell, Vol. 157, Issue 5 https://doi.org/10.1016/j.cell.2014.03.056	journal	May 2014
Detection of Pol IV/RDR2-dependent transcripts at the genomic scale in Arabidopsis reveals features and regulation of siRNA biogenesis Li, Shaofang; Vandivier, Lee E.; Tu, Bin Genome Research, Vol. 25, Issue 2 https://doi.org/10.1101/gr.182238.114	journal	November 2014
PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesis Mosher, R. A.; Schwach, F.; Studholme, D. Proceedings of the National Academy of Sciences, Vol. 105, Issue 8 https://doi.org/10.1073/pnas.0709632105	journal	February 2008
Messenger RNA Degradation: Beginning at the End van Hoof, Ambro; Parker, Roy Current Biology, Vol. 12, Issue 8 https://doi.org/10.1016/S0960-9822(02)00802-3	journal	April 2002
Spatial and functional relationships among Pol V-associated loci, Pol IV-dependent siRNAs, and cytosine methylation in the Arabidopsis epigenome Wierzbicki, A. T.; Cocklin, R.; Mayampurath, A. Genes & Development, Vol. 26, Issue 16 https://doi.org/10.1101/gad.197772.112	journal	August 2012
Two Components of the RNA-Directed DNA Methylation Pathway Associate with MORC6 and Silence Loci Targeted by MORC6 in Arabidopsis Liu, Zhang-Wei; Zhou, Jin-Xing; Huang, Huan-Wei PLOS Genetics, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pgen.1006026	journal	May 2016
Regulation and function of DNA methylation in plants and animals He, Xin-Jian; Chen, Taiping; Zhu, Jian-Kang Cell Research, Vol. 21, Issue 3 https://doi.org/10.1038/cr.2011.23	journal	February 2011
IDN2 and Its Paralogs Form a Complex Required for RNA–Directed DNA Methylation Zhang, Cui-Jun; Ning, Yong-Qiang; Zhang, Su-Wei PLoS Genetics, Vol. 8, Issue 5 https://doi.org/10.1371/journal.pgen.1002693	journal	May 2012
Deadenylation of mRNA by the CCR4â€“NOT complex in Drosophila: molecular and developmental aspects Temme, Claudia; Simonelig, Martine; Wahle, Elmar Frontiers in Genetics, Vol. 5 https://doi.org/10.3389/fgene.2014.00143	journal	May 2014
Identification of small RNA pathway genes using patterns of phylogenetic conservation and divergence Tabach, Yuval; Billi, Allison C.; Hayes, Gabriel D. Nature, Vol. 493, Issue 7434 https://doi.org/10.1038/nature11779	journal	December 2012
The Arabidopsis Nucleosome Remodeler DDM1 Allows DNA Methyltransferases to Access H1-Containing Heterochromatin Zemach, Assaf; Kim, M. Yvonne; Hsieh, Ping-Hung Cell, Vol. 153, Issue 1 https://doi.org/10.1016/j.cell.2013.02.033	journal	March 2013
Regulation of Alternative Splicing Through Coupling with Transcription and Chromatin Structure Naftelberg, Shiran; Schor, Ignacio E.; Ast, Gil Annual Review of Biochemistry, Vol. 84, Issue 1 https://doi.org/10.1146/annurev-biochem-060614-034242	journal	June 2015
Dicer-independent RNA-directed DNA methylation in Arabidopsis Yang, Dong-Lei; Zhang, Guiping; Tang, Kai Cell Research, Vol. 26, Issue 1 https://doi.org/10.1038/cr.2015.145	journal	December 2015
Intergenic transcription by RNA Polymerase II coordinates Pol IV and Pol V in siRNA-directed transcriptional gene silencing in Arabidopsis Zheng, B.; Wang, Z.; Li, S. Genes & Development, Vol. 23, Issue 24 https://doi.org/10.1101/gad.1868009	journal	November 2009
An Importin β Protein Negatively Regulates MicroRNA Activity in Arabidopsis Wang, Wei; Ye, Ruiqiang; Xin, Ying The Plant Cell, Vol. 23, Issue 10 https://doi.org/10.1105/tpc.111.091058	journal	October 2011
Four putative SWI2/SNF2 chromatin remodelers have dual roles in regulating DNA methylation in Arabidopsis Yang, Dong-Lei; Zhang, Guiping; Wang, Lili Cell Discovery, Vol. 4, Issue 1 https://doi.org/10.1038/s41421-018-0056-8	journal	October 2018
RNA Polymerase IV Directs Silencing of Endogenous DNA Herr, A. J. Science, Vol. 308, Issue 5718 https://doi.org/10.1126/science.1106910	journal	April 2005
RNA polymerase V-dependent small RNAs in Arabidopsis originate from small, intergenic loci including most SINE repeats Lee, Tzuu-fen; Gurazada, Sai Guna Ranjan; Zhai, Jixian Epigenetics, Vol. 7, Issue 7 https://doi.org/10.4161/epi.20290	journal	July 2012
RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA Böhmdorfer, Gudrun; Rowley, M. Jordan; Kuciński, Jan The Plant Journal, Vol. 79, Issue 2 https://doi.org/10.1111/tpj.12563	journal	June 2014
The SET Domain Proteins SUVH2 and SUVH9 Are Required for Pol V Occupancy at RNA-Directed DNA Methylation Loci Liu, Zhang-Wei; Shao, Chang-Rong; Zhang, Cui-Jun PLoS Genetics, Vol. 10, Issue 1 https://doi.org/10.1371/journal.pgen.1003948	journal	January 2014
NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation He, X. -J.; Hsu, Y. -F.; Pontes, O. Genes & Development, Vol. 23, Issue 3 https://doi.org/10.1101/gad.1765209	journal	February 2009
GW182 Proteins Directly Recruit Cytoplasmic Deadenylase Complexes to miRNA Targets Braun, Joerg E.; Huntzinger, Eric; Fauser, Maria Molecular Cell, Vol. 44, Issue 1 https://doi.org/10.1016/j.molcel.2011.09.007	journal	October 2011
In Vitro Transcription Activities of Pol IV, Pol V, and RDR2 Reveal Coupling of Pol IV and RDR2 for dsRNA Synthesis in Plant RNA Silencing Haag, Jeremy R.; Ream, Thomas S.; Marasco, Michelle Molecular Cell, Vol. 48, Issue 5 https://doi.org/10.1016/j.molcel.2012.09.027	journal	December 2012
Cytoplasmic Assembly and Selective Nuclear Import of Arabidopsis ARGONAUTE4/siRNA Complexes Ye, Ruiqiang; Wang, Wei; Iki, Taichiro Molecular Cell, Vol. 46, Issue 6 https://doi.org/10.1016/j.molcel.2012.04.013	journal	June 2012
Genetic and Functional Diversification of Small RNA Pathways in Plants Xie, Zhixin; Johansen, Lisa K.; Gustafson, Adam M. PLoS Biology, Vol. 2, Issue 5 https://doi.org/10.1371/journal.pbio.0020104	journal	February 2004
The highways and byways of mRNA decay Garneau, Nicole L.; Wilusz, Jeffrey; Wilusz, Carol J. Nature Reviews Molecular Cell Biology, Vol. 8, Issue 2 https://doi.org/10.1038/nrm2104	journal	February 2007
NOT2 Proteins Promote Polymerase II–Dependent Transcription and Interact with Multiple MicroRNA Biogenesis Factors in Arabidopsis Wang, Lulu; Song, Xianwei; Gu, Lianfeng The Plant Cell, Vol. 25, Issue 2 https://doi.org/10.1105/tpc.112.105882	journal	February 2013
Noncoding Transcription by RNA Polymerase Pol IVb/Pol V Mediates Transcriptional Silencing of Overlapping and Adjacent Genes Wierzbicki, Andrzej T.; Haag, Jeremy R.; Pikaard, Craig S. Cell, Vol. 135, Issue 4 https://doi.org/10.1016/j.cell.2008.09.035	journal	November 2008
AtCCR4a and AtCCR4b are Involved in Determining the Poly(A) Length of Granule-bound starch synthase 1 Transcript and Modulating Sucrose and Starch Metabolism in Arabidopsis thaliana Suzuki, Yuya; Arae, Toshihiro; Green, Pamela J. Plant and Cell Physiology, Vol. 56, Issue 5 https://doi.org/10.1093/pcp/pcv012	journal	January 2015
A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis Zhai, Jixian; Bischof, Sylvain; Wang, Haifeng Cell, Vol. 163, Issue 2 https://doi.org/10.1016/j.cell.2015.09.032	journal	October 2015
An Rrp6-like Protein Positively Regulates Noncoding RNA Levels and DNA Methylation in Arabidopsis Zhang, Huiming; Tang, Kai; Qian, Weiqiang Molecular Cell, Vol. 54, Issue 3 https://doi.org/10.1016/j.molcel.2014.03.019	journal	May 2014

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