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Title: Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis

Journal Article · · New Phytologist
DOI:https://doi.org/10.1111/nph.15050· OSTI ID:1630145
 [1];  [2];  [3];  [3];  [4];  [5];  [4];  [3];  [6];  [7];  [8];  [5];  [9];  [10];  [2];  [5];  [4];  [3]
  1. Department of Plant Biology Michigan State University East Lansing MI 48823 USA, DOE Great Lakes Bioenergy Research Center Michigan State University East Lansing MI 48823 USA
  2. Department of Biochemistry University of Cambridge Cambridge CB2 1QW UK
  3. Carlsberg Research Laboratory 1799 Copenhagen V Denmark
  4. Complex Carbohydrate Research Center University of Georgia 315 Riverbend Road Athens GA 30602 USA, BioEnergy Science Center Oak Ridge National Lab Laboratory Oak Ridge TN 37831 USA
  5. Complex Carbohydrate Research Center University of Georgia 315 Riverbend Road Athens GA 30602 USA
  6. Department of Plant and Environmental Sciences University of Copenhagen 1971 Frederiksberg C Denmark
  7. Botanical Institute Department of Biological Sciences Universität zu Köln Köln D‐50674 Germany
  8. BGI‐Shenzhen Beishan Industrial Zone Yantian District Shenzhen 518083 China
  9. Department of Plant Biology Michigan State University East Lansing MI 48823 USA, DOE Great Lakes Bioenergy Research Center Michigan State University East Lansing MI 48823 USA, Department of Biochemistry and Molecular Biology Michigan State University East Lansing MI 48824 USA
  10. Joint BioEnergy Institute Emeryville CA 94608 USA, Environmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA
+ Show Author Affiliations

Summary Insights into the evolution of plant cell walls have important implications for comprehending these diverse and abundant biological structures. In order to understand the evolving structure–function relationships of the plant cell wall, it is imperative to trace the origin of its different components. The present study is focused on plant 1,4‐β‐xylan, tracing its evolutionary origin by genome and transcriptome mining followed by phylogenetic analysis, utilizing a large selection of plants and algae. It substantiates the findings by heterologous expression and biochemical characterization of a charophyte alga xylan synthase. Of the 12 known gene classes involved in 1,4‐β‐xylan formation, XYS 1/ IRX 10 in plants, IRX 7, IRX 8, IRX 9, IRX 14 and GUX occurred for the first time in charophyte algae. An XYS 1/ IRX 10 ortholog from Klebsormidium flaccidum , designated K. flaccidum XYLAN SYNTHASE ‐1 ( Kf XYS 1), possesses 1,4‐β‐xylan synthase activity, and 1,4‐β‐xylan occurs in the K. flaccidum cell wall. These data suggest that plant 1,4‐β‐xylan originated in charophytes and shed light on the origin of one of the key cell wall innovations to occur in charophyte algae, facilitating terrestrialization and emergence of polysaccharide‐based plant cell walls.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1630145
Alternate ID(s):
OSTI ID: 1425434
Journal Information:
New Phytologist, Journal Name: New Phytologist Vol. 218 Journal Issue: 3; ISSN 0028-646X
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 43 works
Citation information provided by
Web of Science

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
biosynthesis
cell wall
evolution
IRX10
Klebsormidium flaccidum
Klebsormidium nitens
xylan
XYS1