Effects of fungal pretreatment and steam explosion pretreatment on enzymatic saccharification of plant biomass

Journal Article · Wed Dec 20 04:00:00 EST 1995 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.260480621· OSTI ID:162990

Sawada, Tatsuro; Nakamura, Yoshitoshi; Kobayashi, Fumihisa ^[1]; Kuwahara, Masaaki; Watanabe, Takashi ^[2]

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1. Kanazawa Univ. (Japan)
2. Kyoto Univ., Uji (Japan). Wood Research Inst.

The effects of consecutive treatments by a lignin-degrading fungus Phanerochate chrysosporium and by steam explosion for the enzymatic saccharification of plant biomass were studied experimentally, and the optimal operational conditions for obtaining the maximum saccharification were evaluated. Beech wood-meal was treated by the fungus for 98 days and then by high steam temperatures of 170--230 C with steaming times of 0--10 min. The treatment of the wood-meal by fungus prior to steam explosion enhanced the saccharification of wood-meal. The treated wood-meal was separated into holocellulose, water soluble material, methanol soluble lignin, and Klason lignin. The saccharification decreased linearly with the increase in the amount of Klason lignin. It was estimated by the equation for the saccharification of exploded wood-meal expressed as a function of steam temperature and steaming time that the maximum saccharification of wood-meal was obtained by consecutive treatments such as fungal treatment for 28 days and then steam explosion at a steam temperature of 215 C and a steaming time of 6.5 min.

• Sponsoring Organization: USDOE
• OSTI ID: 162990
• Journal Information: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 6 Vol. 48; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULOSE
ENZYMATIC HYDROLYSIS
EQUATIONS
FUNGI
GROWTH