Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Col11a1 Regulates Bone Microarchitecture during Embryonic Development

Journal Article · · Journal of Developmental Biology
DOI:https://doi.org/10.3390/jdb3040158· OSTI ID:1629773
 [1];  [2];  [3];  [3];  [4];  [3]
  1. Boise State University, ID (United States); DOE/OSTI
  2. Brigham Young University, Provo, UT (United States)
  3. Boise State University, ID (United States)
  4. Brigham Young University, Provo, UT (United States); Roseman University, South Jordan, UT (United States)
+ Show Author Affiliations
Collagen XI alpha 1 (Col11a1) is an extracellular matrix molecule required for embryonic development with a role in both nucleating the formation of fibrils and regulating the diameter of heterotypic fibrils during collagen fibrillar assembly. Although found in many different tissues throughout the vertebrate body, Col11a1 plays an essential role in endochondral ossification. To further understand the function of Col11a1 in the process of bone formation, we compared skeletal mineralization in wild-type (WT) mice and Col11a1-deficient mice using X-ray microtomography (micro-CT) and histology. Changes in trabecular bone microstructure were observed and are presented here. Additionally, changes to the periosteal bone collar of developing long bones were observed and resulted in an increase in thickness in the case of Col11a1-deficient mice compared to WT littermates. Vertebral bodies were incompletely formed in the absence of Col11a1. The data demonstrate that Col11a1 depletion results in alteration to newly-formed bone and is consistent with a role for Col11a1 in mineralization. These findings indicate that expression of Col11a1 in the growth plate and perichondrium is essential for trabecular bone and bone collar formation during endochondral ossification. The observed changes to mineralized tissues further define the function of Col11a1.
Research Organization:
Boise State Univ., ID (United States)
Sponsoring Organization:
Idaho State Board of Education Higher Education Research Council; Lori and Duane Stueckle; M. J. Murdock Charitable Trust; National Institutes of Health; St. Luke’s Regional Medical Center; USDOE Office of Science (SC), Advanced Scientific Computing Research (ASCR). Scientific Discovery through Advanced Computing (SciDAC)
Grant/Contract Number:
SC0021399
OSTI ID:
1629773
Journal Information:
Journal of Developmental Biology, Journal Name: Journal of Developmental Biology Journal Issue: 4 Vol. 3; ISSN 2221-3759
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

References (60)

Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committee journal December 1987
End labeling studies of fragmented DNA in the Avian growth plate: Evidence of apoptosis in terminally differentiated chondrocytes journal December 1995
Spatial and temporal expression of fibril-forming minor collagen genes (types V and XI) during fracture healing journal September 1997
Conditional deletion of Indian hedgehog from collagen type 2α1-expressing cells results in abnormal endochondral bone formation journal December 2005
Normal production of cartilage glycosaminoglycan in mice homozygous for the chondrodysplasia gene journal June 1976
Pulmonary hypoplasia in chondrodystrophic mice journal June 1986
The type XI collagenopathies journal October 1998
A fibrillar collagen gene, Col11a1, is essential for skeletal morphogenesis journal February 1995
Fibrochondrogenesis Results from Mutations in the COL11A1 Type XI Collagen Gene journal November 2010
Collagen 11a1 is indirectly activated by lymphocyte enhancer-binding factor 1 (Lef1) and negatively regulates osteoblast maturation journal May 2008
Linking Hematopoiesis to Endochondral Skeletogenesis through Analysis of Mice Transgenic for Collagen X journal June 2002
Auditory function associated with Col11a1 haploinsufficiency in chondrodysplasia (cho) mice journal January 2003
Spatio-temporal expression of FGFR 1, 2 and 3 genes during human embryo-fetal ossification journal September 1998
The ability of three-dimensional structural indices to reflect mechanical aspects of trabecular bone journal July 1999
Chondrodysplasia of gene knockout mice for aggrecan and link protein journal May 2002
Osteoclasts derived from haematopoietic stem cells journal February 1980
Developmental regulation of the growth plate journal May 2003
BMP-1-mediated proteolytic processing of alternatively spliced isoforms of collagen type XI journal December 2003
Co-expression of collagens II and XI and alternative splicing of exon 2 of collagen II in several developing human tissues journal September 1993
Bone Marrow, Cytokines, and Bone Remodeling — Emerging Insights into the Pathophysiology of Osteoporosis journal February 1995
Alternative Exon Splicing within the Amino-terminal Nontriple-helical Domain of the Rat Pro-1(XI) Collagen Chain Generates Multiple Forms of the mRNA Transcript Which Exhibit Tissue-dependent Variation journal April 1995
Alternative mRNA Processing Occurs in the Variable Region of the Pro-1(XI) and Pro-2(XI) Collagen Chains journal April 1995
Collagen XI Nucleates Self-assembly and Limits Lateral Growth of Cartilage Fibrils journal March 2000
Structural Organization of Distinct Domains within the Non-collagenous N-terminal Region of Collagen Type XI journal April 2000
A new Chondrodystrophic Mutant in mice journal March 1971
A Rapid One-Step Trichrome Stain* journal July 1950
Colorectal carcinogenesis is associated with stromal expression of COL11A1 and COL5A2 journal June 2001
FGF signaling pathways in endochondral and intramembranous bone development and human genetic disease journal June 2002
Bone Development journal November 2000
Developmentally Regulated Alternative Splicing of the α1(XI) Collagen Chain: Spatial and Temporal Segregation of Isoforms in the Cartilage of Fetal Rat Long Bones journal June 2000
Maintenance of chondroitin sulfation balance by chondroitin-4-sulfotransferase 1 is required for chondrocyte development and growth factor signaling during cartilage morphogenesis journal September 2005
Ultrastructural studies of cartilage matrix in mice homozygous for chondrodysplasia. journal January 1981
Structural and Associative Properties of Cartilage Matrix Constituents in Mice with Hereditary Chondrodysplasia ( cho ) journal January 1981
Cellular and Molecular Events During Embryonic Bone Development journal January 1989
Thoracic Volume Reduction as a Mechanism for Pulmonary Hypoplasia in Chondrodystrophic Mice journal January 1990
Temporal and spatial expression of alternative splice-forms of the α1(XI) collagen gene in fetal rat cartilage journal September 1998
Identification of the cartilage α 1(XI) chain in type V collagen from bovine bone journal January 1989
Linking Hematopoiesis to Endochondral Skeletogenesis through Analysis of Mice Transgenic for Collagen X journal June 2002
Concerted modulation of alpha 1(XI) and alpha 2(V) collagen mRNAs in bovine vascular smooth muscle cells. journal December 1991
Type XI collagen is a heterotrimer with the composition (1 alpha, 2 alpha, 3 alpha) retaining non-triple-helical domains. journal August 1987
Differential expression of a cysteine-rich domain in the amino-terminal propeptide of type II (cartilage) procollagen by alternative splicing of mRNA. journal June 1990
Isolation and characterization of the chains of type V/type XI collagen present in bovine vitreous journal May 1993
Pro-alpha 1(XI) collagen. Structure of the amino-terminal propeptide and expression of the gene in tumor cell lines. journal April 1990
Auditory function associated with Col11a1 haploinsufficiency in chondrodysplasia (cho) mice journal January 2003
Spatio-temporal expression of FGFR 1, 2 and 3 genes during human embryo-fetal ossification journal September 1998
The ability of three-dimensional structural indices to reflect mechanical aspects of trabecular bone journal July 1999
Collagen XI chain misassembly in cartilage of the chondrodysplasia (cho) mouse journal October 2007
Chondrodysplasia of gene knockout mice for aggrecan and link protein journal May 2002
Investigation of cell lineage in bone using a chimaera of chick and quail embryonic tissue journal November 1975
A new method for the model‐independent assessment of thickness in three‐dimensional images journal January 1997
Bone Marrow, Cytokines, and Bone Remodeling — Emerging Insights into the Pathophysiology of Osteoporosis journal February 1995
The 10+4 microfibril structure of thin cartilage fibrils journal November 2006
Isolated osteoclasts resorb the organic and inorganic components of bone. journal April 1986
A Functional Polymorphism in COL11A1, Which Encodes the α1 Chain of Type XI Collagen, Is Associated with Susceptibility to Lumbar Disc Herniation journal December 2007
Collagen family of proteins journal October 1991
Clinical and molecular analysis of UAE fibrochondrogenesis patients expands the phenotype and reveals two COL11A1 homozygous null mutations journal July 2011
The human rhabdomyosarcoma cell line A204 lays down a highly insoluble matrix composed mainly of alpha1 type-XI and alpha2 type-V collagen chains journal November 1992
Marching cubes: A high resolution 3D surface construction algorithm journal August 1987
Hemivertebra: Prenatal Diagnosis, Incidence and Characteristics journal January 2005
Bone Morphogenetic Proteins in Bone Stimulate Osteoclasts and Osteoblasts During Bone Development journal May 2006

Cited By (7)

Matrisome Profiling During Intervertebral Disc Development And Ageing journal September 2017
The Role of Col11a1 Expression During Cartilage Development dissertation May 2017
Understanding chondrodysplasia ( cho ): A comprehensive review of cho as an animal model of birth defects, disorders, and molecular mechanisms journal February 2019
Extracellular RNA in aging: Extracellular RNA in aging journal August 2016
The mechanical impact of col11a2 loss on joints; col11a2 mutant zebrafish show changes to joint development and function, which leads to early-onset osteoarthritis
  • Lawrence, Elizabeth A.; Kague, Erika; Aggleton, Jessye A.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 373, Issue 1759 https://doi.org/10.1098/rstb.2017.0335
journal September 2018
The mechanical impact of col11a2 loss on joints; col11a2 mutant zebrafish show changes to joint development and function, which leads to early onset osteoarthritis
  • Lawrence, Elizabeth A.; Kague, Erika; Aggleton, Jessye A.
  • Philosophical Transactions of the Royal Society B: Biological Sciences https://doi.org/10.1101/302307
journal July 2018
Endoplasmic Reticulum Stress and Unfolded Protein Response in Cartilage Pathophysiology; Contributing Factors to Apoptosis and Osteoarthritis journal March 2017

Similar Records

Loss of PiT-2 results in abnormal bone development and decreased bone mineral density and length in mice
Journal Article · Sun Jan 14 23:00:00 EST 2018 · Biochemical and Biophysical Research Communications · OSTI ID:23127530
Tenascin-x deficiency mimics ehlers-danlos syndrome in mice through alteration of collagen deposition
Journal Article · Thu Feb 28 23:00:00 EST 2002 · Nature Genetics · OSTI ID:821322
New insights to the role of aryl hydrocarbon receptor in bone phenotype and in dioxin-induced modulation of bone microarchitecture and material properties
Journal Article · Thu Nov 14 23:00:00 EST 2013 · Toxicology and Applied Pharmacology · OSTI ID:22285503

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Col11a1
chondrodysplasia
collagen XI
mineralization
x-ray microtomography