Comparison of xylose fermentation by two high-performance engineered strains of Saccharomyces cerevisiae

Li, Xin; Park, Annsea; Estrela, Raissa; Kim, Soo-Rin; Jin, Yong-Su; Cate, Jamie H. D.

doi:10.1016/j.btre.2016.01.003

Comparison of xylose fermentation by two high-performance engineered strains of Saccharomyces cerevisiae

Journal Article · Fri Jan 22 00:00:00 EST 2016 · Biotechnology Reports (Online)

DOI:https://doi.org/10.1016/j.btre.2016.01.003· OSTI ID:1629588

Li, Xin ^[1]; Park, Annsea ^[2]; Estrela, Raissa ^[2]; Kim, Soo-Rin ^[3]; Jin, Yong-Su ^[4]; Cate, Jamie H. D. ^[5]

Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; DOE/OSTI
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of Illinois at Urbana-Champaign, IL (United States). Inst. for Genomic Biology
Univ. of Illinois at Urbana-Champaign, IL (United States). Inst. for Genomic Biology; Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Food and Science and Human Nutrition
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division

Economical biofuel production from plant biomass requires the conversion of both cellulose and hemicellulose in the plant cell wall. The best industrial fermentation organism, the yeast Saccharomyces cerevisiae, has been developed to utilize xylose by heterologously expressing either a xylose reductase/ xylitol dehydrogenase (XR/XDH) pathway or a xylose isomerase (XI) pathway. Although it has been proposed that the optimal means for fermenting xylose into biofuels would use XI instead of the XR/XDH pathway, no clear comparison of the best publicly-available yeast strains engineered to use XR/XDH or XI has been published. We therefore compared two of the best-performing engineered yeast strains in the public domain—one using the XR/XDH pathway and another using XI—in anaerobic xylose fermentations. We find that, regardless of conditions, the strain using XR/XDH has substantially higher productivity compared to the XI strain. By contrast, the XI strain has better yields in nearly all conditions tested.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Energy Biosciences Institute; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1629588

Journal Information:: Biotechnology Reports (Online), Journal Name: Biotechnology Reports (Online) Journal Issue: C Vol. 9; ISSN 2215-017X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production Cunha, Joana T.; Soares, Pedro O.; Baptista, Sara L. Bioengineered, Vol. 11, Issue 1 https://doi.org/10.1080/21655979.2020.1801178	journal	January 2020
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RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization HamediRad, Mohammad; Lian, Jiazhang; Li, Hejun Biotechnology and Bioengineering, Vol. 115, Issue 6 https://doi.org/10.1002/bit.26570	journal	March 2018
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From agro-industrial wastes to single cell oils: a step towards prospective biorefinery Diwan, Batul; Parkhey, Piyush; Gupta, Pratima Folia Microbiologica, Vol. 63, Issue 5 https://doi.org/10.1007/s12223-018-0602-7	journal	April 2018
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