H3-thymidine derivative pools in relation to macronuclear DNA synthesis in Tetrahymena pyriformis

Stone, G. E.; Miller, O. L.; Prescott, D. M.

doi:10.1083/jcb.25.2.171

H³-thymidine derivative pools in relation to macronuclear DNA synthesis in Tetrahymena pyriformis

Journal Article · Sat May 01 00:00:00 EDT 1965 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.25.2.171· OSTI ID:1629498

Stone, G. E. ^[1]; Miller, O. L. ^[2]; Prescott, D. M. ^[2]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division; DOE/OSTI
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division

The formation of a soluble H³-thymidine derivative pool has been examined in Tetrahymena pyriformis as a function of macronuclear DNA synthesis during the cell life cycle. An autoradiographic technique which allows the detection of water-soluble materials within a cell has shown that these cells do not take up and retain exogenous H³-thymidine during G1 or G2. Uptake of H³-thymidine is restricted to the S period of the cell cycle. Additional autoradiographic experiments show, however, that a soluble pool of H3-thymidine derivatives persists from the end of one DNA synthesis period to the beginning of the next synthesis period in the subsequent cell cycle. Since this persisting pool cannot be labeled with H³-thymidine, the pool does not turn over during non-S periods.

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: US Atomic Energy Commission (AEC); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1629498

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 2 Vol. 25; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

References (3)

Studies on the biosynthesis of deoxyribonucleic acid by extracts of mammalian cells IV. The phosphorylation of thymidine Weissman, S. M.; Smellie, R. M. S.; Paul, J. Biochimica et Biophysica Acta, Vol. 45 https://doi.org/10.1016/0006-3002(60)91430-x	journal	January 1960
Molecular Facets of Mitotic Regulation, ii. Factors Underlying the Removal of Thymidine Kinase Hotta, Yasou; Stern, Herbert Proceedings of the National Academy of Sciences, Vol. 49, Issue 6 https://doi.org/10.1073/pnas.49.6.861	journal	June 1963
Cell Division and dna Synthesis in Tetrahymena Pyriformis Deprived of Essential Amino Acids Stone, G. E.; Prescott, D. M. Journal of Cell Biology, Vol. 21, Issue 2 https://doi.org/10.1083/jcb.21.2.275	journal	May 1964

Cited By (2)

Sex, Hormones and Neurogenesis in the Hippocampus: Hormonal Modulation of Neurogenesis and Potential Functional Implications Galea, L. A. M.; Wainwright, S. R.; Roes, M. M. Journal of Neuroendocrinology, Vol. 25, Issue 11 https://doi.org/10.1111/jne.12070	journal	October 2013
Sex, hormones, and neurogenesis in the hippocampus : Hormonal modulation of neurogenesis and potential functional implications Galea, Liisa A. M.; Wainwright, Steven R.; Duarte-Guterman, Paula The University of British Columbia https://doi.org/10.14288/1.0369055	text	January 2018

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