Studies on Amphibian Yolk: 2. The Isolation of Yolk Platelets from the Eggs of Rana pipiens
Journal Article
·
· Journal of Cell Biology
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); DOE/OSTI
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Previous methods which employed simple sucrose or salt solutions to isolate yolk platelets have failed to preserve their superficial layer, and the preparations obtained generally exhibit some contamination when observed with the light or electron microscope. When yolk platelets are suspended in a sucrose-polyvinylpyrrolidinone medium, however, they remain relatively intact and their superficial layer is not lost to the medium. A method, which takes advantage of this fact, is described for the isolation of frog (Rana pipiens) yolk platelets which are free from nuclear contamination and practically free from cytoplasmic contamination. Further, after such platelets are treated with distilled water, the superficial layer is no longer seen and a new dense and granular matrix is frequently found surrounding the crystalline main body. The significance of this and other observations concerning the effects of calcium and ethylenediamine tetraacetate are discussed.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Grant/Contract Number:
- AC05-00OR22725
- OSTI ID:
- 1629450
- Journal Information:
- Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 1 Vol. 18; ISSN 0021-9525
- Publisher:
- Rockefeller University PressCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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