Incorporation of nucleotides into nuclei of fixed cells by DNA polymerase

Journal Article · Fri Apr 01 00:00:00 EST 1966 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.29.1.21· OSTI ID:1629441

von Borstel, R. C. ^[1]; Prescott, D. M. ^[2]; Bollum, F. J. ^[2]

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1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division; DOE/OSTI
2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division

The enzyme calf thymus polymerase requires denatured or single-stranded DNA as a primer for DNA synthesis and is inactive on native DNA preparations. The enzyme and tritium-labeled deoxyribonucleoside triphosphates were incubated with alcohol-fixed and Carnoy-fixed tissue preparations to see if primer DNA could be found in several types of cells undergoing DNA synthesis. In all cases, low-pH controls were prepared for comparison. Priming activity was not found in nuclei that had been fixed in alcohol. Priming activity was found in cell nuclei that had been fixed with an acid fixative or had been treated at a low pH prior to treatment with the enzyme reaction mixture.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Fusion Energy Sciences (FES)
• Grant/Contract Number: AC05-00OR22725
• OSTI ID: 1629441
• Journal Information: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 1 Vol. 29; ISSN 0021-9525
• Publisher: Rockefeller University Press
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES