tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay

Peschek, Jirka; Walter, Peter

doi:10.7554/elife.44199

tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay

Journal Article · Tue Jun 25 00:00:00 EDT 2019 · eLife

DOI:https://doi.org/10.7554/elife.44199· OSTI ID:1628902

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Univ. of California, San Francisco, CA (United States). Howard Hughes Medical Inst. Dept. of Biochemistry and Biophysics; DOE/OSTI
Univ. of California, San Francisco, CA (United States). Howard Hughes Medical Inst. Dept. of Biochemistry and Biophysics

Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that catalyzes exon-exon ligation during tRNA biogenesis and the non-conventional splicing of HAC1 mRNA during the unfolded protein response (UPR). The UPR regulates the protein folding capacity of the endoplasmic reticulum (ER). ER stress activates Ire1, an ER-resident kinase/RNase, which excises an intron from HAC1 mRNA followed by exon-exon ligation by Trl1. The spliced product encodes for a potent transcription factor that drives the UPR. Here we report the crystal structure of Trl1 RNA ligase domain from Chaetomium thermophilum at 1.9 Å resolution. Structure-based mutational analyses uncovered kinetic competition between RNA ligation and degradation during HAC1 mRNA splicing. Incompletely processed HAC1 mRNA is degraded by Xrn1 and the Ski/exosome complex. We establish cleaved HAC1 mRNA as endogenous substrate for ribosome-associated quality control. We conclude that mRNA decay and surveillance mechanisms collaborate in achieving fidelity of non-conventional mRNA splicing during the UPR.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1628902

Alternate ID(s):: OSTI ID: 1844650

Journal Information:: eLife, Journal Name: eLife Vol. 8; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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