Kinetic mechanism of coupled binding in sodium-aspartate symporter GltPh

Oh, SeCheol; Boudker, Olga

doi:10.7554/elife.37291

Kinetic mechanism of coupled binding in sodium-aspartate symporter GltPh

Journal Article · Wed Sep 26 00:00:00 EDT 2018 · eLife

DOI:https://doi.org/10.7554/elife.37291· OSTI ID:1628885

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Cornell Univ., Ithaca, NY (United States). Weill Medical College. Dept. of Physiology & Biophysics; DOE/OSTI
Cornell Univ., Ithaca, NY (United States). Weill Medical College. Dept. of Physiology & Biophysics; Howard Hughes Medical Inst., Chevy Chase, MD (United States)

Many secondary active membrane transporters pump substrates against concentration gradients by coupling their uptake to symport of sodium ions. Symport requires the substrate and ions to be always transported together. Cooperative binding of the solutes is a key mechanism contributing to coupled transport in the sodium and aspartate symporter from Pyrococcus horikoshii GltPh. Here, we describe the kinetic mechanism of coupled binding for GltPh in the inward facing state. The first of the three coupled sodium ions, binds weakly and slowly, enabling the protein to accept the rest of the ions and the substrate. The last ion binds tightly, but is in rapid equilibrium with solution. Its release is required for the complex disassembly. Thus, the first ion serves to ‘open the door’ for the substrate, the last ion ‘locks the door’ once the substrate is in, and one ion contributes to both events.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1628885

Journal Information:: eLife, Journal Name: eLife Vol. 7; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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