A family of photoswitchable NMDA receptors

Berlin, Shai; Szobota, Stephanie; Reiner, Andreas; Carroll, Elizabeth C.; Kienzler, Michael A.; Guyon, Alice; Xiao, Tong; Trauner, Dirk; Isacoff, Ehud Y.

doi:10.7554/elife.12040

A family of photoswitchable NMDA receptors

Journal Article · Mon Feb 29 23:00:00 EST 2016 · eLife

DOI:https://doi.org/10.7554/elife.12040· OSTI ID:1628842

Berlin, Shai ^[1]; Szobota, Stephanie ^[2]; Reiner, Andreas ^[2]; Carroll, Elizabeth C. ^[2]; Kienzler, Michael A. ^[2]; Guyon, Alice ^[3]; Xiao, Tong ^[4]; Trauner, Dirk ^[5]; ^[6]

Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; DOE/OSTI
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Université de Nice Sophia Antipolis, Nice (France). Institut de Pharmacologie Moléculaire et Cellulaire,
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Univ. of Munich (Germany). Center of Integrated Protein Science. Dept. of Chemistry
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Helen Wills Neuroscience Inst.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division

NMDA receptors, which regulate synaptic strength and are implicated in learning and memory, consist of several subtypes with distinct subunit compositions and functional properties. To enable spatiotemporally defined, rapid and reproducible manipulation of function of specific subtypes, we engineered a set of photoswitchable GluN subunits ('LiGluNs'). Photo-agonism of GluN2A or GluN2B elicits an excitatory drive to hippocampal neurons that can be shaped in time to mimic synaptic activation. Photo-agonism of GluN2A at single dendritic spines evokes spine-specific calcium elevation and expansion, the morphological correlate of LTP. Photo-antagonism of GluN2A alone, or in combination with photo-antagonism of GluN1a, reversibly blocks excitatory synaptic currents, prevents the induction of long-term potentiation and prevents spine expansion. In addition, photo-antagonism in vivo disrupts synaptic pruning of developing retino-tectal projections in larval zebrafish. By providing precise and rapidly reversible optical control of NMDA receptor subtypes, LiGluNs should help unravel the contribution of specific NMDA receptors to synaptic transmission, integration and plasticity.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1628842

Journal Information:: eLife, Journal Name: eLife Vol. 5; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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