Metagenomes and metatranscriptomes from the L4 long-term coastal monitoring station in the Western English Channel

Gilbert, Jack A.; Meyer, Folker; Schriml, Lynn; Joint, Ian R.; Mühling, Martin; Field, Dawn

doi:10.4056/sigs.1202536

Metagenomes and metatranscriptomes from the L4 long-term coastal monitoring station in the Western English Channel

Journal Article · Wed Oct 27 00:00:00 EDT 2010 · Standards in Genomic Sciences

DOI:https://doi.org/10.4056/sigs.1202536· OSTI ID:1628652

Gilbert, Jack A. ^[1]; Meyer, Folker ^[2]; Schriml, Lynn ^[3]; Joint, Ian R. ^[4]; Mühling, Martin ^[5]; Field, Dawn ^[6]

Plymouth Marine Lab., Plymouth (United Kingdom); Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, IL (United States); DOE/OSTI
Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, IL (United States)
Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States). School of Medicine
Plymouth Marine Lab., Plymouth (United Kingdom)
Technical Univ. Bergakademie, Freiberg (Germany). IÖZ - Interdisciplinary Centre for Ecology
NERC Centre for Ecology and Hydrology, Oxford (United Kingdom)

Both metagenomic data and metatranscriptomic data were collected from surface water (0- 2m) of the L4 sampling station (50.2518 N, 4.2089 W), which is part of the Western Channel Observatory long-term coastal-marine monitoring station. We previously generated from this area a six-year time series of 16S rRNA V6 data, which demonstrated robust seasonal structure for the bacterial community, with diversity correlated with day length. Here we describe the features of these metagenomes and metatranscriptomes. We generated 8 metagenomes (4.5 million sequences, 1.9 Gbp, average read-length 350 bp) and 7 metatranscriptomes (392,632 putative mRNA-derived sequences, 159 Mbp, average read-length 272 bp) for eight time-points sampled in 2008. These time points represent three seasons (winter, spring, and summer) and include both day and night samples. These data demonstrate the major differences between genetic potential and actuality, whereby genomes follow general seasonal trends yet with surprisingly little change in the functional potential over time; transcripts tended to be far more structured by changes occurring between day and night.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1628652

Journal Information:: Standards in Genomic Sciences, Journal Name: Standards in Genomic Sciences Journal Issue: 2 Vol. 3; ISSN 1944-3277

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
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Metagenomes and metatranscriptomes from the L4 long-term coastal monitoring station in the Western English Channel

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