X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

Hutchison, Christopher D. M.; Cordon-Preciado, Violeta; Morgan, Rhodri; Nakane, Takanori; Ferreira, Josie; Dorlhiac, Gabriel; Sanchez-Gonzalez, Alvaro; Johnson, Allan S.; Fitzpatrick, Ann; Fare, Clyde; Marangos, Jon P.; Yoon, Chun Hong; Hunter, Mark S.; DePonte, Daniel P.; Boutet, Sébastien; Owada, Shigeki; Tanaka, Rie; Tono, Kensuke; Iwata, So; van Thor, Jasper J.

doi:10.3390/ijms18091918

X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

Journal Article · Thu Sep 07 00:00:00 EDT 2017 · International Journal of Molecular Sciences (Online)

DOI:https://doi.org/10.3390/ijms18091918· OSTI ID:1628376

Hutchison, Christopher D. M. ^[1]; Cordon-Preciado, Violeta ^[2]; Morgan, Rhodri ^[2]; Nakane, Takanori ^[3]; Ferreira, Josie ^[2]; Dorlhiac, Gabriel ^[2]; Sanchez-Gonzalez, Alvaro ^[2]; Johnson, Allan S. ^[2]; Fitzpatrick, Ann ^[4]; Fare, Clyde ^[2]; Marangos, Jon P. ^[2]; Yoon, Chun Hong ^[5]; Hunter, Mark S. ^[5]; DePonte, Daniel P. ^[5]; Boutet, Sébastien ^[5]; Owada, Shigeki ^[6]; Tanaka, Rie ^[6]; Tono, Kensuke ^[7]; Iwata, So ^[8]; van Thor, Jasper J. ^[2]

Imperial College, London (United Kingdom); DOE/OSTI
Imperial College, London (United Kingdom)
Univ. of Tokyo (Japan)
Science and Technology Facilities Council (STFC), Oxford (United Kingdom). Diamond Light Source, Ltd.
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
RIKEN SPring-8 Center, Hyogo (Japan)
RIKEN SPring-8 Center, Hyogo (Japan); Japan Synchrotron Radiation Research Institute, Sayo, Hyogo (Japan)
RIKEN SPring-8 Center, Hyogo (Japan); Kyoto Univ. (Japan)

The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on) state at 1.9 Angstrom resolution and the trans (off) state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA) at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS) at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN) injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1628376

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 9 Vol. 18; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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