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Integrated Self-Assembly of the Mms6 Magnetosome Protein to Form an Iron-Responsive Structure

Journal Article · · International Journal of Molecular Sciences (Online)
 [1];  [2];  [3];  [4];  [4];  [2]
  1. Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States). Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology; DOE/OSTI
  2. Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States). Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology
  3. Ames Lab., Ames, IA (United States)
  4. Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States). Dept. of Chemical and Biological Engineering
+ Show Author Affiliations

A common feature of biomineralization proteins is their self-assembly to produce a surface consistent in size with the inorganic crystals that they produce. Mms6, a small protein of 60 amino acids from Magnetospirillum magneticum strain AMB-1 that promotes the in vitro growth of superparamagnetic magnetite nanocrystals, assembles in aqueous solution to form spherical micelles that could be visualized by TEM and AFM. The results reported here are consistent with the view that the N and C-terminal domains interact with each other within one polypeptide chain and across protein units in the assembly. From studies to determine the amino acid residues important for self-assembly, we identified the unique GL repeat in the N-terminal domain with additional contributions from amino acids in other positions, throughout the molecule. Analysis by CD spectroscopy identified a structural change in the iron-binding C-terminal domain in the presence of Fe3+. A change in the intrinsic fluorescence of tryptophan in the N-terminal domain showed that this structural change is transmitted through the protein. Thus, self-assembly of Mms6 involves an interlaced structure of intra- and inter-molecular interactions that results in a coordinated structural change in the protein assembly with iron binding.

Research Organization:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-07CH11358
OSTI ID:
1628368
Journal Information:
International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 7 Vol. 14; ISSN 1422-0067; ISSN IJMCFK
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

References (22)

Structural Changes in a Protein Fragment from Abalone Shell during the Precipitation of Calcium Carbonate journal September 2012
Transmission Electron Microscopy of GroEL, GroES, and the Symmetrical GroEL/ES Complex journal May 1994
Highly repetitive structure and its organization of the silk fibroin gene journal June 1994
Self-Assembly of Bovine β-Casein below the Isoelectric pH journal March 2008
Interfacial Properties and Iron Binding to Bacterial Proteins That Promote the Growth of Magnetite Nanocrystals: X-ray Reflectivity and Surface Spectroscopy Studies journal February 2012
Effect of Temperature on Self-Assembly of Bovine β-Casein above and below Isoelectric pH. Structural Analysis by Cryogenic-Transmission Electron Microscopy and Small-Angle X-ray Scattering journal April 2008
I-TASSER: a unified platform for automated protein structure and function prediction journal March 2010
Self-assembly of tunable protein suprastructures from recombinant oleosin journal July 2012
Comparative Genome Analysis of Four Magnetotactic Bacteria Reveals a Complex Set of Group-Specific Genes Implicated in Magnetosome Biomineralization and Function journal April 2007
I-TASSER server for protein 3D structure prediction journal January 2008
Protein-Mediated Synthesis of Uniform Superparamagnetic Magnetite Nanocrystals journal April 2007
Self-Assembly of Thermally Responsive Nanoparticles of a Genetically Encoded Peptide Polymer by Drug Conjugation journal December 2012
Self-Assembly of Thermally Responsive Nanoparticles of a Genetically Encoded Peptide Polymer by Drug Conjugation journal December 2012
Highly repetitive structure and its organization of the silk fibroin gene journal June 1994
Characterization of a Bacillus megaterium strain with metal bioremediation potential and in silico discovery of novel cadmium binding motifs in the regulator, CadC
  • Kumari, Weerasingha Mudiyanselage Nilmini H.; Thiruchittampalam, Shalini; Weerasinghe, Mahinagoda Siril Samantha
  • Applied Microbiology and Biotechnology, Vol. 105, Issue 6 https://doi.org/10.1007/s00253-021-11193-2
journal March 2021
Biomineralization proteins: from vertebrates to bacteria journal May 2012
Self-Assembly and Biphasic Iron-Binding Characteristics of Mms6, A Bacterial Protein That Promotes the Formation of Superparamagnetic Magnetite Nanoparticles of Uniform Size and Shape journal December 2011
Cobalt Ferrite Nanocrystals: Out-Performing Magnetotactic Bacteria journal October 2007
MMS6 Protein Regulates Crystal Morphology during Nano-sized Magnetite Biomineralization in Vivo journal December 2010
A Novel Protein Tightly Bound to Bacterial Magnetic Particles in Magnetospirillum magneticum Strain AMB-1 journal December 2002
A Little Chemistry Helps the Big Get Bigger journal October 2010
Comparative Genome Analysis of Four Magnetotactic Bacteria Reveals a Complex Set of Group-Specific Genes Implicated in Magnetosome Biomineralization and Function journal April 2007

Cited By (8)

Artificial coiled coil biomineralisation protein for the synthesis of magnetic nanoparticles journal June 2019
Core Amino Acid Residues in the Morphology-Regulating Protein, Mms6, for Intracellular Magnetite Biomineralization journal October 2016
Visualization of Iron-Binding Micelles in Acidic Recombinant Biomineralization Protein, MamC journal January 2014
Investigating the ferric ion binding site of magnetite biomineralisation protein Mms6 journal February 2020
The magnetosome model: insights into the mechanisms of bacterial biomineralization journal January 2013
Structure prediction of magnetosome-associated proteins journal January 2014
Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy journal April 2016
Using a biomimetic membrane surface experiment to investigate the activity of the magnetite biomineralisation protein Mms6 journal January 2016

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
Chemistry
Mms6
micelle
structural rearrangement