Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death

Liu, Sijia; Fu, Chunxiang; Gou, Jiqing; Sun, Liang; Huhman, David; Zhang, Yunwei; Wang, Zeng-Yu

doi:10.3389/fpls.2017.00982

Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death

Journal Article · Tue Jun 20 00:00:00 EDT 2017 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2017.00982· OSTI ID:1628283

Liu, Sijia ^[1]; Fu, Chunxiang ^[2]; Gou, Jiqing ^[3]; Sun, Liang ^[4]; Huhman, David ^[5]; Zhang, Yunwei ^[6]; Wang, Zeng-Yu ^[3]

China Agricultural Univ., Beijing (China). National Energy R&D Center for Biomass. Dept. of Grassland Science; The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Forage Improvement Division; DOE/OSTI
Chinese Academy of Sciences (CAS), Qingdao (China). Qingdao Inst. of Bioenergy and Bioprocess Technology
The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Forage Improvement Division; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center
The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Computing Services
The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Plant Biology Division
China Agricultural Univ., Beijing (China). National Energy R&D Center for Biomass. Dept. of Grassland Science

Switchgrass (Panicum virgatum) has been developed into a model lignocellulosic bioenergy crop. Downregulation of caffeic acid O-methyltransferase (COMT), a key enzyme in lignin biosynthesis, has been shown to alter lignification and increase biofuel yield in switchgrass. Methylenetetrahydrofolate reductase (MTHFR) mediates C1 metabolism and provides methyl units consumed by COMT. It was predicted that co-silencing of MTHFR and COMT would impact lignification even more than either of the single genes. However, our results showed that strong downregulation of MTHFR in a COMT-deficient background led to altered plant growth and development, but no significant change in lignin content or composition was found when compared with COMT plants. Another unexpected finding was that the double MTHFR/COMT downregulated plants showed a novel lesion-mimic leaf phenotype. Molecular analyses revealed that the lesion-mimic phenotype was caused by the synergistic effect of MTHFR and COMT genes, with MTHFR playing a predominant role. Microarray analysis showed significant induction of genes related to oxidative and defense responses. The results demonstrated the lack of additive effects of MTHFR and COMT on lignification. Furthermore, this research revealed an unexpected role of the two genes in the modulation of lesion-mimic cell death as well as their synergistic effects on agronomic performance.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1628283

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 8; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death

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