Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins

Knaff, David B.; Sutton, Roger B.

doi:10.3389/fpls.2013.00461

Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins

Journal Article · Tue Jan 01 00:00:00 EST 2013 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2013.00461· OSTI ID:1628248

Knaff, David B. ^[1]; Sutton, Roger B. ^[2]

Texas Tech Univ., Lubbock, TX (United States). Dept. of Chemistry and Biochemistry; Texas Tech Univ., Lubbock, TX (United States). Center for Biotechnology and Genomics; DOE/OSTI
Texas Tech Univ., Lubbock, TX (United States). Health Sciences Center. Dept. of Cell Physiology and Molecular Biophysics; Texas Tech Univ., Lubbock, TX (United States). Center for Membrane Protein Research

Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.

View Accepted Manuscript (DOE)

Research Organization:: Texas Tech Univ., Lubbock, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG03-99ER20346

OSTI ID:: 1628248

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 4; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Plant Sciences
educational platform
glutaredoxin A
mutagenesis
protein structure
synechocystis sp. PCC 6803
tertiary

Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins

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