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Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus

Journal Article · · Frontiers in Microbiology
 [1];  [2];  [3];  [4];  [4];  [3];  [4];  [5];  [5];  [6];  [3]
  1. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology; DOE/OSTI
  2. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology; Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France)
  3. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology
  4. Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France)
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France); Beijing Forestry University, Beijing (China). Beijing Advanced Innovation Center for Tree Breeding by Molecular Design
+ Show Author Affiliations
The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid mycorrhizal (ERM) fungi, the molecular and cellular mechanisms that extend tolerance to the host plant are currently unknown. Here, we show a reduced Cd content in Cd-exposed mycorrhizal roots of Vaccinium myrtillus colonized by a metal tolerant isolate of the fungus Oidiodendron maius as compared to non-mycorrhizal roots. To better understand this phenotype, we applied Next Generation Sequencing technologies to analyze gene expression in V. myrtillus and O. maius Zn grown under normal and Cd-stressed conditions, in the free living and in the mycorrhizal status. The results clearly showed that Cd had a stronger impact on plant gene expression than symbiosis, whereas fungal gene expression was mainly regulated by symbiosis. The higher abundance of transcripts coding for stress related proteins in non-mycorrhizal roots may be related to the higher Cd content. Regulated plant metal transporters have been identified that may play a role in reducing Cd content in mycorrhizal roots exposed to this metal.
Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1628186
Journal Information:
Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 11; ISSN 1664-302X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

Cd tolerance
transcriptomics
Microbiology
Oidiodendron maius
Vaccinium myrtillus
ericoid mycorrhiza