Isotopic Analysis of Sporocarp Protein and Structural Material Improves Resolution of Fungal Carbon Sources

Chen, Janet; Hofmockel, Kirsten S.; Hobbie, Erik A.

doi:10.3389/fmicb.2016.01994

Isotopic Analysis of Sporocarp Protein and Structural Material Improves Resolution of Fungal Carbon Sources

Journal Article · Mon Dec 26 00:00:00 EST 2016 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2016.01994· OSTI ID:1628151

Chen, Janet ^[1]; Hofmockel, Kirsten S. ^[2]; Hobbie, Erik A. ^[3]

Univ. of New Hampshire, Durham, NH (United States). Earth, Oceans and Space, Earth Systems Research Center; FAO/IAEA Agriculture and Biotechnology Labs., Seibersdorf (Austria); DOE/OSTI
Iowa State Univ., Ames, IA (United States). Dept. of Ecology, Evolution, and Organismal Biology; Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL); Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Div.
Univ. of New Hampshire, Durham, NH (United States). Earth, Oceans and Space, Earth Systems Research Center

Fungal acquisition of resources is difficult to assess in the field. To determine whether fungi received carbon from recent plant photosynthate, litter or soil-derived organic (C:N bonded) nitrogen, we examined differences in δ13C among bulk tissue, structural carbon, and protein extracts of sporocarps of three fungal types: saprotrophic fungi, fungi with hydrophobic ectomycorrhizae, or fungi with hydrophilic ectomycorrhizae. Sporocarps were collected from experimental plots of the Duke Free-air CO₂ enrichment experiment during and after CO₂ enrichment. The differential ¹³C labeling of ecosystem pools in CO₂ enrichment experiments was tracked into fungi and provided novel insights into organic nitrogen use. Specifically, sporocarp δ¹³C as well as δ¹⁵N of protein and structural material indicated that fungi with hydrophobic ectomycorrhizae used soil-derived organic nitrogen sources for protein carbon, fungi with hydrophilic ectomycorrhizae used recent plant photosynthates for protein carbon and both fungal groups used photosynthates for structural carbon. Saprotrophic fungi depended on litter produced during fumigation for both protein and structural material.

View Accepted Manuscript (DOE)

Research Organization:: Duke Univ., Durham, NC (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-76RL01830; FG02-95ER62083

OSTI ID:: 1628151

Alternate ID(s):: OSTI ID: 1872717

Report Number(s):: PNNL-SA-122602

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 7; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Isotopic Analysis of Sporocarp Protein and Structural Material Improves Resolution of Fungal Carbon Sources

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