Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica

Carr, Stephanie A.; Orcutt, Beth N.; Mandernack, Kevin W.; Spear, John R.

doi:10.3389/fmicb.2015.00872

Title: Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica

Journal Article · Wed Aug 26 00:00:00 EDT 2015 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2015.00872· OSTI ID:1628139

Carr, Stephanie A. ^[1]; Orcutt, Beth N. ^[2]; Mandernack, Kevin W. ^[3]; Spear, John R. ^[1]

Colorado School of Mines, Golden, Colorado (United States). Department of Civil and Environmental Engineering
Bigelow Laboratory for Ocean Sciences, East Boothbay, ME (United States)
Indiana University – Purdue University Indianapolis, Indianapolis, IN (United States). Department of Earth Sciences

Bacteria belonging to the newly classified candidate phylum “Atribacteria” (formerly referred to as “OP9” and “JS1”) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure culture representatives. In this study of deep sediment from Antarctica’s Adélie Basin, collected during Expedition 318 of the Integrated Ocean Drilling Program (IODP), Atribacteria-related sequences of the 16S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance with depth throughout the methane-rich zones. To better understand the metabolic potential of Atribacteria within this environment, and to compare with phylogenetically distinct Atribacteria from non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected from 97.41 m below the seafloor from IODP Hole U1357C. As observed for non-marine Atribacteria, a partial single cell genome suggests a heterotrophic metabolism, with Atribacteria potentially producing fermentation products such as acetate, ethanol, and CO₂. These products may in turn support methanogens within the sediment microbial community and explain the frequent occurrence of Atribacteria in anoxic methane-rich sediments. This first report of a single cell genome from deep sediment broadens the known diversity within the Atribacteria phylum and highlights the potential role of Atribacteria in carbon cycling in deep sediment.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1628139

Journal Information:: Frontiers in Microbiology, Vol. 6; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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