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Fractionation of sulfur isotopes by Desulfovibrio vulgaris mutants lacking hydrogenases or type I tetraheme cytochrome c3

Journal Article · · Frontiers in Microbiology
 [1];  [2];  [3];  [3];  [2];  [2]
  1. Northwestern Univ., Evanston, IL (United States). Dept. of Earth and Planetary Sciences; Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Earth, Atmospheric and Planetary Sciences; DOE/OSTI
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Earth, Atmospheric and Planetary Sciences
  3. Univ. of Missouri, Columbia, MO (United States). Dept. of Biochemsitry
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The sulfur isotope effect produced by sulfate reducing microbes is commonly used to trace biogeochemical cycles of sulfur and carbon in aquatic and sedimentary environments. To test the contribution of intracellular coupling between carbon and sulfur metabolisms to the overall magnitude of the sulfur isotope effect, this study compared sulfur isotope fractionations by mutants of Desulfovibrio vulgaris Hildenborough. We tested mutant strains lacking one or two periplasmic (Hyd, Hyn-1, Hyn-2, and Hys) or cytoplasmic hydrogenases (Ech and CooL), and a mutant lacking type I tetraheme cytochrome (TpI-c3). In batch culture, wild-type D. vulgaris and its hydrogenase mutants had comparable growth kinetics and produced the same sulfur isotope effects. This is consistent with the reported redundancy of hydrogenases in D. vulgaris. However, the TpI-c3 mutant (cycA) exhibited slower growth and sulfate reduction rates in batch culture, and produced more H2 and an approximately 50% larger sulfur isotope effect, compared to the wild type. The magnitude of sulfur isotope fractionation in the CycA deletion strain, thus, increased due to the disrupted coupling of the carbon oxidation and sulfate reduction pathways. In continuous culture, wild-type D. vulgaris and the CycA mutant produced similar sulfur isotope effects, underscoring the influence of environmental conditions on the relative contribution of hydrogen cycling to the electron transport. The large sulfur isotope effects associated with the non-ideal stoichiometry of sulfate reduction in this study imply that simultaneous fermentation and sulfate reduction may be responsible for some of the large naturally-occurring sulfur isotope effects. Overall, mutant strains provide a powerful tool to test the effect of specific redox proteins and pathways on sulfur isotope fractionation.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1628090
Journal Information:
Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 4; ISSN 1664-302X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (7)

The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20 posted_content May 2014
Sulfur Isotope Effects of Dissimilatory Sulfite Reductase journal December 2015
Transhydrogenase and Growth Substrate Influence Lipid Hydrogen Isotope Ratios in Desulfovibrio alaskensis G20 journal June 2016
Influence of Phosphorus and Cell Geometry on the Fractionation of Sulfur Isotopes by Several Species of Desulfovibrio during Microbial Sulfate Reduction journal May 2017
Biosynthesized Iron Sulfide Nanocluster Enhanced Anodic Current Generation by Sulfate Reducing Bacteria in Microbial Fuel Cells journal October 2018
Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community journal July 2019
The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20 journal October 2014

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Microbiology
electron transport
hydrogen cycling
isotope effect
mutation
sulfate reduction