Community Structure and Function of High-Temperature Chlorophototrophic Microbial Mats Inhabiting Diverse Geothermal Environments

Klatt, Christian G.; Inskeep, William P.; Herrgard, Markus J.; Jay, Zackary J.; Rusch, Douglas B.; Tringe, Susannah G.; Niki Parenteau, M.; Ward, David M.; Boomer, Sarah M.; Bryant, Donald A.; Miller, Scott R.

doi:10.3389/fmicb.2013.00106

Community Structure and Function of High-Temperature Chlorophototrophic Microbial Mats Inhabiting Diverse Geothermal Environments

Journal Article · Tue Jan 01 00:00:00 EST 2013 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2013.00106· OSTI ID:1628088

Klatt, Christian G. ^[1]; Inskeep, William P. ^[2]; Herrgard, Markus J. ^[3]; Jay, Zackary J. ^[2]; Rusch, Douglas B. ^[4]; Tringe, Susannah G. ^[5]; Niki Parenteau, M. ^[6]; Ward, David M. ^[2]; Boomer, Sarah M. ^[7]; Bryant, Donald A. ^[8]; Miller, Scott R. ^[9]

Montana State Univ., Bozeman, MT (United States). Dept. of Land Resources and Environmental Sciences; Montana State Univ., Bozeman, MT (United States). Thermal Biology Inst.; DOE/OSTI
Montana State Univ., Bozeman, MT (United States). Dept. of Land Resources and Environmental Sciences; Montana State Univ., Bozeman, MT (United States). Thermal Biology Inst.
Technical Univ. of Denmark, Horsholm (Denmark). Novo Nordisk Foundation Center for Biosustainability
Indiana Univ., Bloomington, IN (United States). Center for Genomics and Bioinformatics
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Search for Extraterrestrial Intelligence Inst., Mountain View, CA (United States); NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States)
Western Oregon Univ., Monmouth, OR (United States)
Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology; Montana State Univ., Bozeman, MT (United States). Dept. of Chemistry and Biochemistry
Univ. of Montana, Missoula, MT (United States). Dept. of Biological Sciences

Six phototrophic microbial mat communities from different geothermal springs (YNP) were studied using metagenome sequencing and geochemical analyses. The primary goals of this work were to determine differences in community composition of high-temperature phototrophic mats distributed across the Yellowstone geothermal ecosystem, and to identify metabolic attributes of predominant organisms present in these communities that may correlate with environmental attributes important in niche differentiation. Random shotgun metagenome sequences from six phototrophic communities (average ~53 Mbp/site) were subjected to multiple taxonomic, phylogenetic, and functional analyses. All methods, including G + C content distribution, MEGAN analyses, and oligonucleotide frequency based clustering, provided strong support for the dominant community members present in each site. Cyanobacteria were only observed in non-sulfidic sites; de novo assemblies were obtained for Synechococcus-like populations at Chocolate Pots (CP_7) and Fischerella-like populations at White Creek (WC_6). Chloroflexi-like sequences (esp. Roseiflexus and/or Chloroflexus spp.) were observed in all six samples and contained genes involved in bacteriochlorophyll biosynthesis and the 3-hydroxypropionate carbon fixation pathway. Other major sequence assemblies were obtained for a Chlorobiales population from CP_7 (proposed family Thermochlorobacteriaceae), and an anoxygenic, sulfur-oxidizing Thermochromatium-like (Gamma-proteobacteria) population from Bath Lake Vista Annex (BLVA_20). Additional sequence coverage is necessary to establish more complete assemblies of other novel bacteria in these sites (e.g., Bacteroidetes and Firmicutes); however, current assemblies suggested that several of these organisms play important roles in heterotrophic and fermentative metabolisms. Definitive linkages were established between several of the dominant phylotypes present in these habitats and important functional processes such as photosynthesis, carbon fixation, sulfur oxidation, and fermentation.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1628088

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 4; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Life in Hot Spring Microbial Mats Located in the Trans-Mexican Volcanic Belt: A 16S/18S rRNA Gene and Metagenomic Analysis Prieto-Barajas, Cristina M.; Alcaraz, Luis D.; Valencia-Cantero, Eduardo Geomicrobiology Journal, Vol. 35, Issue 8 https://doi.org/10.1080/01490451.2018.1454555	journal	April 2018
Chloroflexus islandicus sp. nov., a thermophilic filamentous anoxygenic phototrophic bacterium from a geyser Gaisin, Vasil A.; Kalashnikov, Alexander M.; Grouzdev, Denis S. International Journal of Systematic and Evolutionary Microbiology, Vol. 67, Issue 5 https://doi.org/10.1099/ijsem.0.001820	journal	May 2017
Adaptive and acclimative responses of cyanobacteria to far-red light: Responses of cyanobacteria to far-red light Gan, Fei; Bryant, Donald A. Environmental Microbiology, Vol. 17, Issue 10 https://doi.org/10.1111/1462-2920.12992	journal	August 2015
The role of biology in planetary evolution: cyanobacterial primary production in low-oxygen Proterozoic oceans: Primary production in Proterozoic oceans Hamilton, Trinity L.; Bryant, Donald A.; Macalady, Jennifer L. Environmental Microbiology, Vol. 18, Issue 2 https://doi.org/10.1111/1462-2920.13118	journal	December 2015
Divergence with gene flow in a population of thermophilic bacteria: a potential role for spatially varying selection Wall, Christopher A.; Koniges, Gregory J.; Miller, Scott R. Molecular Ecology, Vol. 23, Issue 14 https://doi.org/10.1111/mec.12812	journal	June 2014
In Situ Hydrogen Dynamics in a Hot Spring Microbial Mat during a Diel Cycle Revsbech, Niels Peter; Trampe, Erik; Lichtenberg, Mads Applied and Environmental Microbiology, Vol. 82, Issue 14 https://doi.org/10.1128/aem.00710-16	journal	May 2016
Multi-species biofilms in ecology, medicine, and biotechnology Nozhevnikova, A. N.; Botchkova, E. A.; Plakunov, V. K. Microbiology, Vol. 84, Issue 6 https://doi.org/10.1134/s0026261715060107	journal	November 2015
Comparative Analysis of Microbial Diversity Across Temperature Gradients in Hot Springs From Yellowstone and Iceland Podar, Peter T.; Yang, Zamin; Björnsdóttir, Snædís H. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.01625	journal	July 2020
Occurrence of Far-Red Light Photoacclimation (FaRLiP) in Diverse Cyanobacteria Gan, Fei; Shen, Gaozhong; Bryant, Donald Life, Vol. 5, Issue 1 https://doi.org/10.3390/life5010004	journal	December 2014

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