The Microbial Ferrous Wheel in a Neutral pH Groundwater Seep

Roden, Eric E.; McBeth, Joyce M.; Blöthe, Marco; Percak-Dennett, Elizabeth M.; Fleming, Emily J.; Holyoke, Rebecca R.; Luther, George W.; Emerson, David; Schieber, Juergen

doi:10.3389/fmicb.2012.00172

The Microbial Ferrous Wheel in a Neutral pH Groundwater Seep

Journal Article · Tue May 22 00:00:00 EDT 2012 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2012.00172· OSTI ID:1628063

Roden, Eric E. ^[1]; McBeth, Joyce M. ^[2]; Blöthe, Marco ^[3]; Percak-Dennett, Elizabeth M. ^[4]; Fleming, Emily J. ^[2]; Holyoke, Rebecca R. ^[5]; Luther, George W. ^[5]; Emerson, David ^[2]; Schieber, Juergen ^[6]

University of Wisconsin, Madison, WI (United States); DOE/OSTI
Bigelow Laboratory for Ocean Sciences, East Boothbay, ME (United States)
University of Wisconsin, Madison, WI (United States); Geozentrum Hannover (Germany)
University of Wisconsin, Madison, WI (United States)
University of Delaware, Lewes, DE (United States)
Indiana University, Bloomington, IN (United States)

Evidence for microbial Fe redox cycling was documented in a circumneutral pH groundwater seep near Bloomington, Indiana. Geochemical and microbiological analyses were conducted at two sites, a semi-consolidated microbial mat and a floating puffball structure. In situ voltammetric microelectrode measurements revealed steep opposing gradients of O₂ and Fe(II) at both sites, similar to other groundwater seep and sedimentary environments known to support microbial Fe redox cycling. The puffball structure showed an abrupt increase in dissolved Fe(II) just at its surface (~5 cm depth), suggesting an internal Fe(II) source coupled to active Fe(III) reduction. Most probable number enumerations detected microaerophilic Fe(II)-oxidizing bacteria (FeOB) and dissimilatory Fe(III)-reducing bacteria (FeRB) at densities of 10² to 10⁵ cells mL^-1 in samples from both sites. In vitro Fe(III) reduction experiments revealed the potential for immediate reduction (no lag period) of native Fe(III) oxides. Conventional full-length 16S rRNA gene clone libraries were compared with high throughput barcode sequencing of the V1, V4, or V6 variable regions of 16S rRNA genes in order to evaluate the extent to which new sequencing approaches could provide enhanced insight into the composition of Fe redox cycling microbial community structure.The composition of the clone libraries suggested a lithotroph-dominated microbial community centered around taxa related to known FeOB (e.g., Gallionella, Sideroxydans, Aquabacterium). Sequences related to recognized FeRB (e.g., Rhodoferax, Aeromonas, Geobacter, Desulfovibrio) were also well-represented. Overall, sequences related to known FeOB and FeRB accounted for 88 and 59% of total clone sequences in the mat and puffball libraries, respectively. Taxa identified in the barcode libraries showed partial overlap with the clone libraries, but were not always consistent across different variable regions and sequencing platforms. However, the barcode libraries provided confirmation of key clone library results (e.g., the predominance of Betaproteobacteria) and an expanded view of lithotrophic microbial community composition.

View Accepted Manuscript (DOE)

Research Organization:: University of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: National Science Foundation (NSF); Office of Naval Research; USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI ID:: 1628063

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 3; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Microbial iron-redox cycling in subsurface environments Roden, Eric E. Biochemical Society Transactions, Vol. 40, Issue 6 https://doi.org/10.1042/bst20120202	journal	November 2012
Relating Microbial Community Structure and Geochemistry in Deep Regolith Developed on Volcaniclastic Rock in the Luquillo Mountains, Puerto Rico Liermann, Laura J.; Albert, Istvan; Buss, Heather L. Geomicrobiology Journal, Vol. 32, Issue 6 https://doi.org/10.1080/01490451.2014.964885	journal	September 2014
Growth of Iron-Oxidizing Bacteria Gallionella ferruginea and Leptothrix cholodnii in Oligotrophic Environments: Ca, Mg, and C as Limiting Factors and G. ferruginea Necromass as C-Source Eggerichs, Tanja; Wiegand, Marlies; Neumann, Karsten Geomicrobiology Journal, Vol. 37, Issue 2 https://doi.org/10.1080/01490451.2019.1686667	journal	November 2019
Iron cycling at corroding carbon steel surfaces Lee, Jason S.; McBeth, Joyce M.; Ray, Richard I. Biofouling, Vol. 29, Issue 10 https://doi.org/10.1080/08927014.2013.836184	journal	September 2013
Bacteria diversity, distribution and insight into their role in S and Fe biogeochemical cycling during black shale weathering: Bacteria in a black shale weathering profile Li, Jiwei; Sun, Weimin; Wang, Shiming Environmental Microbiology, Vol. 16, Issue 11 https://doi.org/10.1111/1462-2920.12536	journal	July 2014
Microbial Iron Oxidation in the Arctic Tundra and Its Implications for Biogeochemical Cycling Emerson, David; Scott, Jarrod J.; Benes, Joshua Applied and Environmental Microbiology, Vol. 81, Issue 23 https://doi.org/10.1128/aem.02832-15	journal	September 2015
Functional Gene Analysis of Freshwater Iron-Rich Flocs at Circumneutral pH and Isolation of a Stalk-Forming Microaerophilic Iron-Oxidizing Bacterium Kato, Shingo; Chan, Clara; Itoh, Takashi Applied and Environmental Microbiology, Vol. 79, Issue 17 https://doi.org/10.1128/aem.03840-12	journal	June 2013
Relating Microbial Community Structure and Geochemistry in Deep Regolith Developed on Volcaniclastic Rock in the Luquillo Mountains, Puerto Rico Liermann, Laura J.; Albert, Istvan; Buss, Heather L. Taylor & Francis https://doi.org/10.6084/m9.figshare.1182612	text	January 2015
Relating microbial community structure and geochemistry in deep regolith developed on volcaniclastic rock in the Luquillo Mountains, Puerto Rico Liermann, Laura J.; Albert, Istvan; Buss, Heather L. Taylor & Francis https://doi.org/10.6084/m9.figshare.1182612.v1	text	January 2015
Relating Microbial Community Structure and Geochemistry in Deep Regolith Developed on Volcaniclastic Rock in the Luquillo Mountains, Puerto Rico Liermann, Laura J.; Albert, Istvan; Buss, Heather L. Taylor & Francis https://doi.org/10.6084/m9.figshare.1182612.v2	text	January 2015

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