Genotyping by Sequencing of 393 Sorghum bicolor BTx623 × IS3620C Recombinant Inbred Lines Improves Sensitivity and Resolution of QTL Detection

Kong, WenQian; Kim, Changsoo; Zhang, Dong; Guo, Hui; Tan, Xu; Jin, Huizhe; Zhou, Chengbo; Shuang, Lan-shuan; Goff, Valorie; Sezen, Uzay; Pierce, Gary; Compton, Rosana; Lemke, Cornelia; Robertson, Jon; Rainville, Lisa; Auckland, Susan; Paterson, Andrew H.

doi:10.1534/g3.118.200173

Genotyping by Sequencing of 393 Sorghum bicolor BTx623 × IS3620C Recombinant Inbred Lines Improves Sensitivity and Resolution of QTL Detection

Journal Article · Thu May 31 00:00:00 EDT 2018 · G3

DOI:https://doi.org/10.1534/g3.118.200173· OSTI ID:1627958

^[1]; Kim, Changsoo ^[2]; Zhang, Dong ^[2]; Guo, Hui ^[2]; Tan, Xu ^[2]; Jin, Huizhe ^[2]; Zhou, Chengbo ^[2]; Shuang, Lan-shuan ^[2]; Goff, Valorie ^[2]; Sezen, Uzay ^[2]; Pierce, Gary ^[2]; Compton, Rosana ^[2]; Lemke, Cornelia ^[2]; Robertson, Jon ^[2]; Rainville, Lisa ^[2]; Auckland, Susan ^[2]; ^[2]

Univ. of Georgia, Athens, GA (United States). Plant Genome Mapping Lab.; DOE/OSTI
Univ. of Georgia, Athens, GA (United States). Plant Genome Mapping Lab.

We describe a genetic map with a total of 381 bins of 616 genotyping by sequencing (GBS)- based SNP markers in a F6-F8 recombinant inbred line (RIL) population of 393 individuals derived from crossing S. bicolor BTx623 to S. bicolor IS3620C, a guinea line substantially diverged from BTx623. Five segregation distorted regions were found with four showing enrichment for S. bicolor alleles, suggesting possible selection during formation of this RIL population. A quantitative trait locus (QTL) study with this number of individuals, tripled relative to prior studies of this cross, provided resources, validated previous findings, and demonstrated improved power to detect plant height and flowering time related QTL relative to other published studies. An unexpected low correlation between flowering time and plant height permitted us to separate QTL for each trait and provide evidence against pleiotropy. Ten non- random syntenic regions conferring QTL for the same trait suggest that those QTL may represent alleles at genes functioning in the same manner since the 96 million year ago genome duplication that created these syntenic relationships, while syntenic regions conferring QTL for different trait may suggest sub-functionalization after duplication. Collectively, this study provides resources for marker-assisted breeding, as well as a framework for fine mapping and subsequent cloning of major genes for important traits such as plant height and flowering time in sorghum.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-00ER41132

OSTI ID:: 1627958

Journal Information:: G3, Journal Name: G3 Journal Issue: 8 Vol. 8; ISSN 2160-1836

Publisher:: Genetics Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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Supplemental Material for Kong et al., 2018 Kong, WenQian; Kim, Changsoo; Zhang, Dong https://doi.org/10.25387/G3.6304538 The current record is supplemented by this collection	collection	January 2018
Supplemental Material for Kong et al., 2018 Kong, WenQian; Kim, Changsoo; Zhang, Dong https://doi.org/10.25387/g3.6304538.v1 The current record is supplemented by this collection	collection	January 2018

Genotyping by Sequencing of 393 Sorghum bicolor BTx623 × IS3620C Recombinant Inbred Lines Improves Sensitivity and Resolution of QTL Detection

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