Genetic Interactions Between Brassinosteroid-Inactivating P450s and Photomorphogenic Photoreceptors in Arabidopsis thaliana

Sandhu, Kulbir Singh; Hagely, Katherine; Neff, Michael M.

doi:10.1534/g3.112.004580

Genetic Interactions Between Brassinosteroid-Inactivating P450s and Photomorphogenic Photoreceptors in Arabidopsis thaliana

Journal Article · Sat Dec 01 00:00:00 EST 2012 · G3

DOI:https://doi.org/10.1534/g3.112.004580· OSTI ID:1627946

Sandhu, Kulbir Singh ^[1]; Hagely, Katherine ^[2]; Neff, Michael M. ^[3]

Washington State University, Pullman, WA (United States); DOE/OSTI
University of Missouri, Columbia, MO (United States)
Washington State University, Pullman, WA (United States)

Plants use light as a source of information via a suite of photomorphogenic photoreceptors to optimize growth in response to their light environment. Growth-promoting hormones such as brassinosteroids also can modulate many of these responses. BAS1 and SOB7 are brassinosteroid-catabolizing P450s in Arabidopsis thaliana that synergistically/redundantly modulate photomorphogenic traits such as flowering time. The role of BAS1 and SOB7 in photomorphogenesis has been investigated by studying null-mutant genetic interactions with the photoreceptors phyA, phyB, and cry1 with regard to seed germination and flowering time. The removal of BAS1 and/or SOB7 rescued the low germination rate of the phyA-211 phyB9 double-null mutant. With regard to floral induction, bas1-2 and sob7-1 showed a complex set of genetic interactions with photoreceptor-null mutants. Histochemical analysis of transgenic plants harboring BAS1: BAS1-GUS and SOB7:SOB7-GUS translational fusions under the control of their endogenous promoters revealed overlapping and distinct expression patterns. BAS1’s expression in the shoot apex increases during the phase transition from short-to-long-day growth conditions and requires phyB in red light. In summary, BAS1 and SOB7 displayed both simple and complex genetic interactions with the phytochromes in a plant-stage specific manner.

View Accepted Manuscript (DOE)

Research Organization:: Washington State University, Pullman, WA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE

OSTI ID:: 1627946

Journal Information:: G3, Journal Name: G3 Journal Issue: 12 Vol. 2; ISSN 2160-1836

Publisher:: Genetics Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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