Gene Annotation and Drug Target Discovery in Candida albicans with a Tagged Transposon Mutant Collection

Oh, Julia; Fung, Eula; Schlecht, Ulrich; Davis, Ronald W.; Giaever, Guri; St. Onge, Robert P.; Deutschbauer, Adam; Nislow, Corey

doi:10.1371/journal.ppat.1001140

**Gene Annotation and Drug Target Discovery in Candida albicans with a Tagged Transposon Mutant Collection**

Journal Article · Thu Oct 07 00:00:00 EDT 2010 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1001140· OSTI ID:1627897

Oh, Julia ^[1]; Fung, Eula ^[2]; Schlecht, Ulrich ^[2]; Davis, Ronald W. ^[3]; Giaever, Guri ^[4]; St. Onge, Robert P. ^[2]; Deutschbauer, Adam ^[5]; Nislow, Corey ^[6]

Stanford University, CA (United States); DOE/OSTI
Stanford Genome Technology Center, Palo Alto, CA (United States)
Stanford University, CA (United States); Stanford Genome Technology Center, Palo Alto, CA (United States)
University of Toronto, ON (Canada); Donnelley Center for Cellular and Biomolecular Research, Toronto, ON (Canada)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Univ. of Toronto, ON (Canada); Donnelley Center for Cellular and Biomolecular Research, Toronto, ON (Canada)

Candida albicans is the most common human fungal pathogen, causing infections that can be lethal in immunocompromised patients. Although Saccharomyces cerevisiae has been used as a model for C. albicans, it lacks C. albicans’ diverse morphogenic forms and is primarily non-pathogenic. Comprehensive genetic analyses that have been instrumental for determining gene function in S. cerevisiae are hampered in C. albicans, due in part to limited resources to systematically assay phenotypes of loss-of-function alleles. Here, we constructed and screened a library of 3633 tagged heterozygous transposon disruption mutants, using them in a competitive growth assay to examine nutrient- and drug-dependent haploinsufficiency. We identified 269 genes that were haploinsufficient in four growth conditions, the majority of which were condition-specific. These screens identified two new genes necessary for filamentous growth as well as ten genes that function in essential processes. We also screened 57 chemically diverse compounds that more potently inhibited growth of C. albicans versus S. cerevisiae. For four of these compounds, we examined the genetic basis of this differential inhibition. Notably, Sec7p was identified as the target of brefeldin A in C. albicans screens, while S. cerevisiae screens with this compound failed to identify this target. We also uncovered a new C. albicans-specific target, Tfp1p, for the synthetic compound 0136-0228. These results highlight the value of haploinsufficiency screens directly in this pathogen for gene annotation and drug target identification.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Canadian Cancer Society; National Human Genome Research Institute (NHGRI); National Institutes of Health (NIH); Stanford Genome Training Program; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627897

Journal Information:: PLoS Pathogens, Journal Name: PLoS Pathogens Journal Issue: 10 Vol. 6; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (75)

A suite of Gateway ^® cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae Alberti, Simon; Gitler, Aaron D.; Lindquist, Susan Yeast, Vol. 24, Issue 10 https://doi.org/10.1002/yea.1502	journal	October 2007
Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: Regulation by starvation and RAS Gimeno, Carlos J.; Ljungdahl, Per O.; Styles, Cora A. Cell, Vol. 68, Issue 6 https://doi.org/10.1016/0092-8674(92)90079-r	journal	March 1992
Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast Parsons, Ainslie B.; Lopez, Andres; Givoni, Inmar E. Cell, Vol. 126, Issue 3 https://doi.org/10.1016/j.cell.2006.06.040	journal	August 2006
Mechanism-of-Action Determination of GMP Synthase Inhibitors and Target Validation in Candida albicans and Aspergillus fumigatus Rodriguez-Suarez, Roberto; Xu, Deming; Veillette, Karynn Chemistry & Biology, Vol. 14, Issue 10 https://doi.org/10.1016/j.chembiol.2007.09.009	journal	October 2007
PAP Inhibitor with In Vivo Efficacy Identified by Candida albicans Genetic Profiling of Natural Products Jiang, Bo; Xu, Deming; Allocco, John Chemistry & Biology, Vol. 15, Issue 4 https://doi.org/10.1016/j.chembiol.2008.02.016	journal	April 2008
Experimental approaches to identify genetic networks Costanzo, Michael; Giaever, Guri; Nislow, Corey Current Opinion in Biotechnology, Vol. 17, Issue 5 https://doi.org/10.1016/j.copbio.2006.08.005	journal	October 2006
The human commensal yeast, Candida albicans, has an ancient origin Lott, Timothy J.; Fundyga, Ruth E.; Kuykendall, Randall J. Fungal Genetics and Biology, Vol. 42, Issue 5 https://doi.org/10.1016/j.fgb.2005.01.012	journal	May 2005
A Robust Toolkit for Functional Profiling of the Yeast Genome Pan, Xuewen; Yuan, Daniel S.; Xiang, Dong Molecular Cell, Vol. 16, Issue 3 https://doi.org/10.1016/j.molcel.2004.09.035	journal	November 2004
Nonfilamentous C. albicans Mutants Are Avirulent Lo, Hsiu-Jung; Köhler, Julia R.; DiDomenico, Beth Cell, Vol. 90, Issue 5 https://doi.org/10.1016/s0092-8674(00)80358-x	journal	September 1997
Genomic profiling of drug sensitivities via induced haploinsufficiency Giaever, Guri; Shoemaker, Daniel D.; Jones, Ted W. Nature Genetics, Vol. 21, Issue 3 https://doi.org/10.1038/6791	journal	March 1999
Functional profiling of the Saccharomyces cerevisiae genome Giaever, Guri; Chu, Angela M.; Ni, Li Nature, Vol. 418, Issue 6896 https://doi.org/10.1038/nature00935	journal	July 2002
A unique and universal molecular barcode array Pierce, Sarah E.; Fung, Eula L.; Jaramillo, Daniel F. Nature Methods, Vol. 3, Issue 8 https://doi.org/10.1038/nmeth905	journal	July 2006
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures Pierce, Sarah E.; Davis, Ron W.; Nislow, Corey Nature Protocols, Vol. 2, Issue 11 https://doi.org/10.1038/nprot.2007.427	journal	November 2007
Chemogenomic profiling: Identifying the functional interactions of small molecules in yeast Giaever, G.; Flaherty, P.; Kumm, J. Proceedings of the National Academy of Sciences, Vol. 101, Issue 3 https://doi.org/10.1073/pnas.0307490100	journal	January 2004
Structure and Properties of the Vacuolar (H+)-ATPases Forgac, Michael Journal of Biological Chemistry, Vol. 274, Issue 19 https://doi.org/10.1074/jbc.274.19.12951	journal	May 1999
Chemical Genetic Profiling and Characterization of Small-molecule Compounds That Affect the Biosynthesis of Unsaturated Fatty Acids in Candida albicans Xu, Deming; Sillaots, Susan; Davison, John Journal of Biological Chemistry, Vol. 284, Issue 29 https://doi.org/10.1074/jbc.m109.019877	journal	July 2009
An update on antifungal targets and mechanisms of resistance in Candida albicans Akins, Robert A. Medical Mycology, Vol. 43, Issue 4 https://doi.org/10.1080/13693780500138971	journal	January 2005
Nosocomial Bloodstream Infections in US Hospitals: Analysis of 24,179 Cases from a Prospective Nationwide Surveillance Study Wisplinghoff, H.; Bischoff, T.; Tallent, S. M. Clinical Infectious Diseases, Vol. 39, Issue 3 https://doi.org/10.1086/421946	journal	August 2004
Completion of a parasexual cycle in Candida albicans by induced chromosome loss in tetraploid strains Bennett, R. J. The EMBO Journal, Vol. 22, Issue 10 https://doi.org/10.1093/emboj/cdg235	journal	May 2003
Genetic characterization of pathogenic Saccharomyces cerevisiae isolates. McCusker, J. H.; Clemons, K. V.; Stevens, D. A. Genetics, Vol. 136, Issue 4 https://doi.org/10.1093/genetics/136.4.1261	journal	April 1994
Candida albicans Mds3p, a Conserved Regulator of pH Responses and Virulence Identified Through Insertional Mutagenesis Davis, Dana A.; Bruno, Vincent M.; Loza, Lucio Genetics, Vol. 162, Issue 4 https://doi.org/10.1093/genetics/162.4.1573	journal	December 2002
Inparanoid: a comprehensive database of eukaryotic orthologs O'Brien, K. P. Nucleic Acids Research, Vol. 33, Issue Database issue https://doi.org/10.1093/nar/gki107	journal	December 2004
New tools at the Candida Genome Database: biochemical pathways and full-text literature search Skrzypek, Marek S.; Arnaud, Martha B.; Costanzo, Maria C. Nucleic Acids Research, Vol. 38, Issue suppl_1 https://doi.org/10.1093/nar/gkp836	journal	October 2009
A universal TagModule collection for parallel genetic analysis of microorganisms Oh, Julia; Fung, Eula; Price, Morgan N. Nucleic Acids Research, Vol. 38, Issue 14 https://doi.org/10.1093/nar/gkq419	journal	May 2010
Association of an activator with an RNA polymerase II holoenzyme. Hengartner, C. J.; Thompson, C. M.; Zhang, J. Genes & Development, Vol. 9, Issue 8 https://doi.org/10.1101/gad.9.8.897	journal	April 1995
Quantitative phenotyping via deep barcode sequencing Smith, A. M.; Heisler, L. E.; Mellor, J. Genome Research, Vol. 19, Issue 10 https://doi.org/10.1101/gr.093955.109	journal	July 2009
Heterozygosity and functional allelic variation in the Candida albicans efflux pump genes CDR1 and CDR2 Holmes, Ann R.; Tsao, Sarah; Ong, Soo-Wee Molecular Microbiology, Vol. 62, Issue 1 https://doi.org/10.1111/j.1365-2958.2006.05357.x	journal	October 2006
The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes Hillenmeyer, M. E.; Fung, E.; Wildenhain, J. Science, Vol. 320, Issue 5874 https://doi.org/10.1126/science.1150021	journal	April 2008
Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis Winzeler, E. A. Science, Vol. 285, Issue 5429 https://doi.org/10.1126/science.285.5429.901	journal	August 1999
Epidemiology of Invasive Candidiasis: a Persistent Public Health Problem Pfaller, M. A.; Diekema, D. J. Clinical Microbiology Reviews, Vol. 20, Issue 1 https://doi.org/10.1128/cmr.00029-06	journal	January 2007
Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns García-Sánchez, Susana; Aubert, Sylvie; Iraqui, Ismaïl Eukaryotic Cell, Vol. 3, Issue 2 https://doi.org/10.1128/ec.3.2.536-545.2004	journal	April 2004
Interleukin-15 Augments Superoxide Production and Microbicidal Activity of Human Monocytes against Candida albicans Vázquez, Nancy; Walsh, Thomas J.; Friedman, Daphne Infection and Immunity, Vol. 66, Issue 1 https://doi.org/10.1128/iai.66.1.145-150.1998	journal	January 1998
Isolation of the MIG1 Gene fromCandida albicans and Effects of Its Disruption on Catabolite Repression Zaragoza, Oscar; Rodríguez, Cristina; Gancedo, Carlos Journal of Bacteriology, Vol. 182, Issue 2 https://doi.org/10.1128/jb.182.2.320-326.2000	journal	January 2000
A Single-Transformation Gene Function Test in DiploidCandida albicans Enloe, Brian; Diamond, Aviva; Mitchell, Aaron P. Journal of Bacteriology, Vol. 182, Issue 20 https://doi.org/10.1128/jb.182.20.5730-5736.2000	journal	October 2000
Rfg1, a Protein Related to the Saccharomyces cerevisiae Hypoxic Regulator Rox1, Controls Filamentous Growth and Virulence in Candida albicans Kadosh, David; Johnson, Alexander D. Molecular and Cellular Biology, Vol. 21, Issue 7 https://doi.org/10.1128/mcb.21.7.2496-2505.2001	journal	April 2001
Genome-Wide Requirements for Resistance to Functionally Distinct DNA-Damaging Agents Lee, William; St. Onge, Robert P.; Proctor, Michael PLoS Genetics, Vol. 1, Issue 2 https://doi.org/10.1371/journal.pgen.0010024	journal	August 2005
An RNA Transport System in Candida albicans Regulates Hyphal Morphology and Invasive Growth Elson, Sarah L.; Noble, Suzanne M.; Solis, Norma V. PLoS Genetics, Vol. 5, Issue 9 https://doi.org/10.1371/journal.pgen.1000664	journal	September 2009
Genome-Wide Fitness Test and Mechanism-of-Action Studies of Inhibitory Compounds in Candida albicans Xu, Deming; Jiang, Bo; Ketela, Troy PLoS Pathogens, Vol. 3, Issue 6 https://doi.org/10.1371/journal.ppat.0030092	journal	June 2007
Mechanisms of Haploinsufficiency Revealed by Genome-Wide Profiling in Yeast Deutschbauer, Adam M.; Jaramillo, Daniel F.; Proctor, Michael Genetics, Vol. 169, Issue 4 https://doi.org/10.1534/genetics.104.036871	journal	February 2005
Standard Growth Media and Common Techniques for Use with Candida albicans Chauhan, Neeraj; Kruppa, Michael D. Candida albicans https://doi.org/10.1007/978-1-60327-151-6_18	book	January 2009
Candidemia in non-neutropenic critically ill patients: analysis of prognostic factors and assessment of systemic antifungal therapy Nolla-Salas, J.; Sitges-Serra, A.; León-Gil, C. Intensive Care Medicine, Vol. 23, Issue 1 https://doi.org/10.1007/s001340050286	journal	January 1997
Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: Regulation by starvation and RAS Gimeno, Carlos J.; Ljungdahl, Per O.; Styles, Cora A. Cell, Vol. 68, Issue 6 https://doi.org/10.1016/0092-8674(92)90079-R	journal	March 1992
Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS Gimeno, Carlos J.; Ljungdahl, Per O.; Styles, Cora A. Cell, Vol. 68, Issue 6, p. 1077-1090 https://doi.org/10.1016/0962-8924(92)90233-d	journal	April 2004
Nonfilamentous C. albicans Mutants Are Avirulent Lo, Hsiu-Jung; Köhler, Julia R.; DiDomenico, Beth Cell, Vol. 90, Issue 5 https://doi.org/10.1016/S0092-8674(00)80358-X	journal	September 1997
Discovering Modes of Action for Therapeutic Compounds Using a Genome-Wide Screen of Yeast Heterozygotes Lum, Pek Yee; Armour, Christopher D.; Stepaniants, Sergey B. Cell, Vol. 116, Issue 1 https://doi.org/10.1016/S0092-8674(03)01035-3	journal	January 2004
Brefeldin A Acts to Stabilize an Abortive ARF–GDP–Sec7 Domain Protein Complex Peyroche, Anne; Antonny, Bruno; Robineau, Sylviane Molecular Cell, Vol. 3, Issue 3 https://doi.org/10.1016/S1097-2765(00)80455-4	journal	March 1999
Yeast chemical genomics and drug discovery: an update Hoon, Shawn; St. Onge, Robert P.; Giaever, Guri Trends in Pharmacological Sciences, Vol. 29, Issue 10 https://doi.org/10.1016/j.tips.2008.07.006	journal	October 2008
A New Yeast Endosomal SNARE Related to Mammalian Syntaxin 8: A New Yeast Endosomal SNARE Lewis, Michael J.; Pelham, Hugh R. B. Traffic, Vol. 3, Issue 12 https://doi.org/10.1034/j.1600-0854.2002.31207.x	journal	December 2002
Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes Goryshin, Igor Y.; Jendrisak, Jerry; Hoffman, Les M. Nature Biotechnology, Vol. 18, Issue 1 https://doi.org/10.1038/72017	journal	January 2000
Natural history and evolutionary principles of gene duplication in fungi Wapinski, Ilan; Pfeffer, Avi; Friedman, Nir Nature, Vol. 449, Issue 7158 https://doi.org/10.1038/nature06107	journal	September 2007
A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds Ho, Cheuk Hei; Magtanong, Leslie; Barker, Sarah L. Nature Biotechnology, Vol. 27, Issue 4 https://doi.org/10.1038/nbt.1534	journal	April 2009
An integrated platform of genomic assays reveals small-molecule bioactivities Hoon, Shawn; M. Smith, Andrew; Wallace, Iain M. Nature Chemical Biology, Vol. 4, Issue 8 https://doi.org/10.1038/nchembio.100	journal	July 2008
DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray Ooi, Siew Loon; Shoemaker, Daniel D.; Boeke, Jef D. Nature Genetics, Vol. 35, Issue 3 https://doi.org/10.1038/ng1258	journal	October 2003
Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar–coding strategy Shoemaker, Daniel D.; Lashkari, Deval A.; Morris, Don Nature Genetics, Vol. 14, Issue 4 https://doi.org/10.1038/ng1296-450	journal	December 1996
Characterization of the gene family encoding alternative oxidase from Candida albicans Huh, Won-Ki; Kang, Sa-Ouk Biochemical Journal, Vol. 356, Issue 2 https://doi.org/10.1042/0264-6021:3560595	journal	June 2001
Characterization of the gene family encoding alternative oxidase from Candida albicans Huh, Won-Ki; Kang, Sa-Ouk Biochemical Journal, Vol. 356, Issue 2 https://doi.org/10.1042/bj3560595	journal	May 2001
Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery: C. albicans essential gene identification and antifungal drug discovery Roemer, Terry; Jiang, Bo; Davison, John Molecular Microbiology, Vol. 50, Issue 1 https://doi.org/10.1046/j.1365-2958.2003.03697.x	journal	August 2003
The diploid genome sequence of Candida albicans Jones, T.; Federspiel, N. A.; Chibana, H. Proceedings of the National Academy of Sciences, Vol. 101, Issue 19 https://doi.org/10.1073/pnas.0401648101	journal	May 2004
Chemical Genetic Profiling and Characterization of Small-molecule Compounds That Affect the Biosynthesis of Unsaturated Fatty Acids in Candida albicans Xu, Deming; Sillaots, Susan; Davison, John Journal of Biological Chemistry, Vol. 284, Issue 29 https://doi.org/10.1074/jbc.M109.019877	journal	July 2009
A novel allele of HWP1, isolated from a clinical strain of Candida albicans with defective hyphal growth and biofilm formation, has deletions of Gln/Pro and Ser/Thr repeats involved in cellular adhesion Padovan, Ana Carolina; Chaves, Guilherme; Colombo, Arnaldo Medical Mycology https://doi.org/10.1080/13693780802669574	journal	January 2009
Haploinsufficiency-based large-scale forward genetic analysis of filamentous growth in the diploid human fungal pathogen C.albicans Uhl, M. A. The EMBO Journal, Vol. 22, Issue 11 https://doi.org/10.1093/emboj/cdg256	journal	June 2003
Transcriptional profiling of the Candida albicans Ssk1p receiver domain point mutants and their virulence Menon, Veena; De Bernardis, Flavia; Calderone, Richard FEMS Yeast Research, Vol. 8, Issue 5 https://doi.org/10.1111/j.1567-1364.2008.00404.x	journal	August 2008
Molecular Evidence for the Early Colonization of Land by Fungi and Plants Heckman, D. S. Science, Vol. 293, Issue 5532 https://doi.org/10.1126/science.1061457	journal	August 2001
The Genetic Landscape of a Cell Costanzo, M.; Baryshnikova, A.; Bellay, J. Science, Vol. 327, Issue 5964 https://doi.org/10.1126/science.1180823	journal	January 2010
Epidemiology of Invasive Candidiasis: a Persistent Public Health Problem Pfaller, M. A.; Diekema, D. J. Clinical Microbiology Reviews, Vol. 20, Issue 1 https://doi.org/10.1128/CMR.00029-06	journal	January 2007
Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns García-Sánchez, Susana; Aubert, Sylvie; Iraqui, Ismaïl Eukaryotic Cell, Vol. 3, Issue 2 https://doi.org/10.1128/EC.3.2.536-545.2004	journal	April 2004
Interleukin-15 Augments Superoxide Production and Microbicidal Activity of Human Monocytes against Candida albicans Vázquez, Nancy; Walsh, Thomas J.; Friedman, Daphne Infection and Immunity, Vol. 66, Issue 1 https://doi.org/10.1128/IAI.66.1.145-150.1998	journal	January 1998
Rapid Hypothesis Testing with Candida albicans through Gene Disruption with Short Homology Regions Wilson, R. Bryce; Davis, Dana; Mitchell, Aaron P. Journal of Bacteriology, Vol. 181, Issue 6 https://doi.org/10.1128/JB.181.6.1868-1874.1999	journal	March 1999
Isolation of the MIG1 Gene fromCandida albicans and Effects of Its Disruption on Catabolite Repression Zaragoza, Oscar; Rodríguez, Cristina; Gancedo, Carlos Journal of Bacteriology, Vol. 182, Issue 2 https://doi.org/10.1128/JB.182.2.320-326.2000	journal	January 2000
A Single-Transformation Gene Function Test in DiploidCandida albicans Enloe, Brian; Diamond, Aviva; Mitchell, Aaron P. Journal of Bacteriology, Vol. 182, Issue 20 https://doi.org/10.1128/JB.182.20.5730-5736.2000	journal	October 2000
Rfg1, a Protein Related to the Saccharomyces cerevisiae Hypoxic Regulator Rox1, Controls Filamentous Growth and Virulence in Candida albicans Kadosh, David; Johnson, Alexander D. Molecular and Cellular Biology, Vol. 21, Issue 7 https://doi.org/10.1128/MCB.21.7.2496-2505.2001	journal	April 2001
The S-Phase Cyclin Clb5 Promotes rRNA Gene (rDNA) Stability by Maintaining Replication Initiation Efficiency in rDNA Goto, Mayuko; Sasaki, Mariko; Kobayashi, Takehiko Molecular and Cellular Biology, Vol. 41, Issue 5 https://doi.org/10.1128/mcb.00324-20	journal	April 2021
The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase Itoh, Roichi; Saint-Marc, Christelle; Chaignepain, Stéphane BMC Biochemistry, Vol. 4, Issue 1, p. 4 https://doi.org/10.1186/1471-2091-4-4	journal	January 2003
A Robust Toolkit for Functional Profiling of the Yeast Genome Xuewen, Pan,; Philip, Hieter,; Sharon, Sookhai-Mahadeo, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/4jj1-yh05	text	January 2004
A novel allele of HWP1 , isolated from a clinical strain of Candida albicans with defective hyphal growth and biofilm formation, has deletions of Gln/Pro and Ser/Thr repeats involved in cellular adhesion Padovan, Ana Carolina B.; Chaves, Guilherme M.; Colombo, Arnaldo L. Medical Mycology, Vol. 47, Issue 8 https://doi.org/10.3109/13693780802669574	journal	December 2009

Cited By (35)

Candida albicans gains azole resistance by altering sphingolipid composition Gao, Jiaxin; Wang, Haitao; Li, Zeyao Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-06944-1	journal	October 2018
Iterative screening methodology enables isolation of strains with improved properties for a FACS-based screen and increased L-DOPA production Savitskaya, Judy; Protzko, Ryan J.; Li, Francesca-Zhoufan Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-41759-0	journal	April 2019
Genome-wide functional analysis in Candida albicans Motaung, Thabiso E.; Ells, Ruan; Pohl, Carolina H. Virulence, Vol. 8, Issue 8 https://doi.org/10.1080/21505594.2017.1292198	journal	March 2017
Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J. mBio, Vol. 6, Issue 3, Article No. e00306-15 https://doi.org/10.1128/mbio.00306-15	journal	May 2015
Essential Gene Discovery in the Basidiomycete Cryptococcus neoformans for Antifungal Drug Target Prioritization Ianiri, Giuseppe; Idnurm, Alexander mBio, Vol. 6, Issue 2 https://doi.org/10.1128/mbio.02334-14	journal	March 2015
A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis Alfred, Simon E.; Surendra, Anuradha; Le, Chris Genome Biology, Vol. 13, Issue 11 https://doi.org/10.1186/gb-2012-13-11-r105	journal	January 2012
Evidence-Based Annotation of Gene Function in Shewanella oneidensis MR-1 Using Genome-Wide Fitness Profiling across 121 Conditions Deutschbauer, Adam; Price, Morgan N.; Wetmore, Kelly M. PLoS Genetics, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pgen.1002385	journal	November 2011
In Vivo Systematic Analysis of Candida albicans Zn2-Cys6 Transcription Factors Mutants for Mice Organ Colonization Vandeputte, Patrick; Ischer, Françoise; Sanglard, Dominique PLoS ONE, Vol. 6, Issue 10 https://doi.org/10.1371/journal.pone.0026962	journal	October 2011
ESSENTIALS: Software for Rapid Analysis of High Throughput Transposon Insertion Sequencing Data Zomer, Aldert; Burghout, Peter; Bootsma, Hester J. PLoS ONE, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pone.0043012	journal	August 2012
A Versatile Overexpression Strategy in the Pathogenic Yeast Candida albicans: Identification of Regulators of Morphogenesis and Fitness Chauvel, Murielle; Nesseir, Audrey; Cabral, Vitor PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0045912	journal	September 2012
Gymnemic Acids Inhibit Hyphal Growth and Virulence in Candida albicans Vediyappan, Govindsamy; Dumontet, Vincent; Pelissier, Franck PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0074189	journal	September 2013
Candida albicans Sfl1/Sfl2 regulatory network drives the formation of pathogenic microcolonies McCall, Andrew D.; Kumar, Rohitashw; Edgerton, Mira PLOS Pathogens, Vol. 14, Issue 9 https://doi.org/10.1371/journal.ppat.1007316	journal	September 2018
Systematic Complex Haploinsufficiency-Based Genetic Analysis of Candida albicans Transcription Factors: Tools and Applications to Virulence-Associated Phenotypes Glazier, Virginia E.; Murante, Thomas; Koselny, Kristy G3 Genes\|Genomes\|Genetics, Vol. 8, Issue 4 https://doi.org/10.1534/g3.117.300515	journal	April 2018
Evolutionary and Biochemical Aspects of Chemical Stress Resistance in Saccharomyces cerevisiae Venancio, Thiago Motta; Bellieny-Rabelo, Daniel; Aravind, L. Frontiers in Genetics, Vol. 3 https://doi.org/10.3389/fgene.2012.00047	journal	January 2012
Functional toxicology: tools to advance the future of toxicity testing GaytÃ¡n, Brandon D.; Vulpe, Chris D. Frontiers in Genetics, Vol. 5 https://doi.org/10.3389/fgene.2014.00110	journal	May 2014
Mitochondrial Complex V α Subunit Is Critical for Candida albicans Pathogenicity through Modulating Multiple Virulence Properties Li, Shui-Xiu; Song, Yan-Jun; Zhang, Yi-Shan Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.00285	journal	February 2017
Harnessing the power of transposon mutagenesis for antibacterial target identification and evaluation Meredith, Timothy C.; Wang, Hao; Beaulieu, Patrick Mobile Genetic Elements, Vol. 2, Issue 4 https://doi.org/10.4161/mge.21647	journal	July 2012
Molecular genetic techniques for gene manipulation inCandida albicans Xu, Qiu-Rong; Yan, Lan; Lv, Quan-Zhen Virulence, Vol. 5, Issue 4 https://doi.org/10.4161/viru.28893	journal	April 2014
Systems-level antimicrobial drug and drug synergy discovery Roemer, Terry; Boone, Charles Nature Chemical Biology, Vol. 9, Issue 4 https://doi.org/10.1038/nchembio.1205	journal	March 2013
Bugs, drugs and chemical genomics Roemer, Terry; Davies, Julian; Giaever, Guri Nature Chemical Biology, Vol. 8, Issue 1 https://doi.org/10.1038/nchembio.744	journal	December 2011
Yeast: a microbe with macro-implications to antimicrobial drug discovery Balibar, Carl J.; Roemer, Terry Briefings in Functional Genomics, Vol. 15, Issue 2 https://doi.org/10.1093/bfgp/elv038	journal	October 2015
Broad-Spectrum Antifungal Activities and Mechanism of Drimane Sesquiterpenoids Edouarzin, Edruce; Horn, Connor; Paduyal, Anuja Microbial Cell https://doi.org/10.1101/816082	posted_content	October 2019
Transposon insertional mutagenesis in Saccharomyces uvarum reveals trans -acting effects influencing species-dependent essential genes Sanchez, Monica R.; Payen, Celia; Cheong, Frances Genome Research, Vol. 29, Issue 3 https://doi.org/10.1101/gr.232330.117	journal	January 2019
Antifungals discovery: an insight into new strategies to combat antifungal resistance Fuentefria, A. M.; Pippi, B.; Dalla Lana, D. F. Letters in Applied Microbiology, Vol. 66, Issue 1 https://doi.org/10.1111/lam.12820	journal	December 2017
Analysis of a fungus-specific transcription factor family, the Candida albicans zinc cluster proteins, by artificial activation: Artificial activation of a transcription factor family Schillig, Rebecca; Morschhäuser, Joachim Molecular Microbiology, Vol. 89, Issue 5 https://doi.org/10.1111/mmi.12327	journal	July 2013
Exploitation of genomics in fungicide research: current status and future perspectives: Genomics and fungicide research Cools, Hans J.; Hammond-Kosack, Kim E. Molecular Plant Pathology, Vol. 14, Issue 2 https://doi.org/10.1111/mpp.12001	journal	November 2012
Towards an Informative Mutant Phenotype for Every Bacterial Gene Deutschbauer, A.; Price, M. N.; Wetmore, K. M. Journal of Bacteriology, Vol. 196, Issue 20 https://doi.org/10.1128/JB.01836-14	journal	August 2014
Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J. mBio, Vol. 6, Issue 3, Article No. e00306-15 https://doi.org/10.1128/mBio.00306-15	journal	May 2015
Gene Essentiality Analyzed by In Vivo Transposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans Segal, Ella Shtifman; Gritsenko, Vladimir; Levitan, Anton mBio, Vol. 9, Issue 5 https://doi.org/10.1128/mbio.02048-18	journal	October 2018
Antifungal Resistance and New Strategies to Control Fungal Infections Vandeputte, Patrick; Ferrari, Selene; Coste, Alix T. International Journal of Microbiology, Vol. 2012 https://doi.org/10.1155/2012/713687	journal	January 2012
A Large-Scale Complex Haploinsufficiency-Based Genetic Interaction Screen in Candida albicans: Analysis of the RAM Network during Morphogenesis Bharucha, Nike; Chabrier-Roselló, Yeissa; Xu, Tao PLoS Genetics, Vol. 7, Issue 4 https://doi.org/10.1371/journal.pgen.1002058	journal	April 2011
The HOG Pathway Is Critical for the Colonization of the Mouse Gastrointestinal Tract by Candida albicans Prieto, Daniel; Román, Elvira; Correia, Inês PLoS ONE, Vol. 9, Issue 1 https://doi.org/10.1371/journal.pone.0087128	journal	January 2014
Maize Transposable Elements Ac / Ds as Insertion Mutagenesis Tools in Candida albicans Mielich, Kevin; Shtifman-Segal, Ella; Golz, Julia C. G3 Genes\|Genomes\|Genetics, Vol. 8, Issue 4 https://doi.org/10.1534/g3.117.300388	journal	April 2018
Broad-spectrum antifungal activities and mechanism of drimane sesquiterpenoids Edouarzin, Edruce; Horn, Connor; Paudyal, Anuja Microbial Cell, Vol. 7, Issue 6 https://doi.org/10.15698/mic2020.06.719	journal	June 2020
The Impact of Gene Dosage and Heterozygosity on the Diploid Pathobiont Candida albicans Liang, Shen-Huan; Bennett, Richard J. Journal of Fungi, Vol. 6, Issue 1 https://doi.org/10.3390/jof6010010	journal	December 2019

Similar Records

Adaptation to the dietary sugar D-tagatose via genome instability in polyploid Candida albicans cells

Journal Article · Thu Apr 08 20:00:00 EDT 2021 · G3 · OSTI ID:2471774

A role for Candida albicans superoxide dismutase enzymes in glucose signaling

Journal Article · Sun Jan 14 23:00:00 EST 2018 · Biochemical and Biophysical Research Communications · OSTI ID:23127542

CRISPR-GRIT: Guide RNAs with Integrated Repair Templates Enable Precise Multiplexed Genome Editing in the Diploid Fungal Pathogen Candida albicans

Journal Article · Mon Oct 21 20:00:00 EDT 2024 · The CRISPR Journal · OSTI ID:2479302

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Candida albicans
drug screening
gene pool
genomics
haploinsufficiency
heterozygosity
saccharomyces cerevisiae
transposable elements