Characterization of a nontypeable Haemophilus influenzae thermonuclease

Cho, Christine; Chande, Aroon T.; Gakhar, Lokesh; Hunt, Jason; Ketterer, Margaret R.; Apicella, Michael A.

doi:10.1371/journal.pone.0197010

Characterization of a nontypeable Haemophilus influenzae thermonuclease

Journal Article · Thu May 10 00:00:00 EDT 2018 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0197010· OSTI ID:1627862

^[1]; ^[2]; Gakhar, Lokesh ^[3]; Hunt, Jason ^[4]; Ketterer, Margaret R. ^[5]; Apicella, Michael A. ^[3]

Univ. of Iowa, Iowa City, IA (United States); Univ. of Iowa Hospitals, Iowa City, IA (United States); Univ. of Iowa Carver College of Medicine, Iowa City, IA (United States); DOE/OSTI
Georgia Inst. of Technology, Atlanta, GA (United States); IHRC-Georgia Tech Applied Bioinformatics Lab., Atlanta, GA (United States); PanAmerican Bioinformatics Inst., Cali, Valle del Cauca (Columbia)
Univ. of Iowa, Iowa City, IA (United States)
Western Illinois Univ., Macomb, IL (United States)
Univ. of Iowa, Iowa City, IA (United States). Dept. of Microbiology

Nontypeable Haemophilus influenzae (NTHi) has been shown to form biofilms, comprised of extracellular DNA (eDNA), in the middle ear and bronchus during clinical infections. Studies in our laboratory have shown that NTHi possesses a homolog of Staphylococcus aureus thermonuclease (staphylococcal thermonuclease), NTHi nuclease (NTHi Nuc, HI_1296). This enzyme had similar size, heat stability, and divalent cation requirements to those of the staphylococcal homolog as determined by light scattering and circular dichroism spectroscopy. Small angle X-ray scattering (SAXS) analysis suggested an overall shape and substrate-binding site comparable to those of staphylococcal nuclease. However, NTHi Nuc was approximately 25-fold more active in fluorescence resonance energy transfer (FRET) activity assay than staphylococcal thermonuclease. Homology modeling implicates shorter NTHi Nuc loops near the active site for this enhanced activity.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)

Sponsoring Organization:: National Institute of Allergy and Infectious Diseases (NIAID); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627862

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 5 Vol. 13; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Characterization of a nontypeable Haemophilus influenzae thermonuclease

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